Manami Oya

Melanocortin System Activates Carotid Body Arterial Chemoreceptors in Hypertension

Audrys G. Pauža, Pratik C. Thakkar, Xin Ming Shen, Igor Simões Assunção Felippe et autres

BACKGROUND: The internal milieu of the body is controlled by a system of interoceptors coupled to motor outflows that drive compensatory adaptive responses. These include the arterial chemoreceptors, best known for sensing arterial oxygen. In cardiometabolic diseases, such as essential hypertension, the …

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Melanocortin system activates carotid body arterial chemoreceptors in hypertension

Audrys G. Pauža, Pratik C. Thakkar, Xin Ming Shen, Igor Simões Assunção Felippe et autres

Abstract Background The body’s internal milieu is controlled by a system of interoceptors coupled to motor outflows that drive compensatory adaptive responses. These include the arterial chemoreceptors, best known for sensing arterial oxygen. In cardiometabolic diseases, such as essential hypertension, the carotid …

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Age-related ciliopathy: Obesogenic shortening of melanocortin-4 receptor-bearing neuronal primary cilia

Manami Oya, Yoshiki Miyasaka, Yoshiko Nakamura, Miyako Tanaka et autres

Obesity is often associated with aging. However, the mechanism of age-related obesity is unknown. The melanocortin-4 receptor (MC4R) mediates leptin-melanocortin anti-obesity signaling in the hypothalamus. Here, we discovered that MC4R-bearing primary cilia of hypothalamic neurons progressively shorten with age in rats, correlating …

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Combi-CRISPR: combination of NHEJ and HDR provides efficient and precise plasmid-based knock-ins in mice and rats

Kazuto Yoshimi, Yuichiro Oka, Yoshiki Miyasaka, Yuko Kotani et autres

CRISPR-Cas9 are widely used for gene targeting in mice and rats. The non-homologous end-joining (NHEJ) repair pathway, which is dominant in zygotes, efficiently induces insertion or deletion (indel) mutations as gene knockouts at targeted sites, whereas gene knock-ins (KIs) via homology-directed repair …

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Low glucose-induced ghrelin secretion is mediated by an ATP-sensitive potassium channel

Manami Oya, Tetsuya Kitaguchi, Kazuki Harada, Rika Numano et autres

Ghrelin is synthesized in X/A-like cells of the gastric mucosa, which plays an important role in the regulation of energy homeostasis. Although ghrelin secretion is known to be induced by neurotransmitters or hormones or by nutrient sensing in the ghrelin-secreting cells themselves, …

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Vesicular nucleotide transporter is involved in ATP storage of secretory lysosomes in astrocytes

Manami Oya, Tetsuya Kitaguchi, Yu Yanagihara, Rika Numano et autres

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Effects of sodium arsenite on neurite outgrowth and glutamate AMPA receptor expression in mouse cortical neurons

Fumihiko Maekawa, Takashi Tsuboi, Manami Oya, Kyaw Htet Aung et autres

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Extracellular calcium influx activates adenylate cyclase 1 and potentiates insulin secretion in MIN6 cells

Tetsuya Kitaguchi, Manami Oya, Yoshiko Wada, Takashi Tsuboi et autres

Intracellular cAMP and Ca(2+) are important second messengers that regulate insulin secretion in pancreatic β-cells; however, the molecular mechanism underlying their mutual interaction for exocytosis is not fully understood. In the present study, we investigated the interplay between intracellular cAMP and Ca(2+) …

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The G Protein-coupled Receptor Family C Group 6 Subtype A (GPRC6A) Receptor Is Involved in Amino Acid-induced Glucagon-like Peptide-1 Secretion from GLUTag Cells

Manami Oya, Tetsuya Kitaguchi, Ramona Pais, Frank M. Reimann et autres

Although amino acids are dietary nutrients that evoke the secretion of glucagon-like peptide 1 (GLP-1) from intestinal L cells, the precise molecular mechanism(s) by which amino acids regulate GLP-1 secretion from intestinal L cells remains unknown. Here, we show that the G …

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Role of Doc2α in dense-core vesicle exocytosis in PC12 cells revealed by live cell imaging

Takashi Tsuboi, Yasunori Mori, Hideki Matsui, Ryo Aoki et autres

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Amino acid taste receptor regulates insulin secretion in pancreatic β-cell line MIN6 cells

Manami Oya, Hideyuki Suzuki, Yuichiro Watanabe, Moritoshi Sato et autres

Amino acids such as L-glutamate and L-arginine are potent stimuli for insulin secretion from pancreatic β-cells. However, the precise molecular mechanisms of amino acid-induced insulin secretion have only partly understood. In this study, we show here that mouse pancreatic β-cell line MIN6 …

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Duration of fusion pore opening and the amount of hormone released are regulated by myosin II during kiss-and-run exocytosis

Ryo Aoki, Tetsuya Kitaguchi, Manami Oya, Yu Yanagihara et autres

Since the fusion pore of the secretory vesicle is resealed before complete dilation during 'kiss-and-run' exocytosis, their cargoes are not completely released. Although the transient fusion pore is kept open for several seconds, the precise mechanisms that control fusion pore maintenance, and …

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