Yoshiki Miyasaka

SOX2 terminates trophectoderm competence in inner cell mass by closing trophectoderm enhancers

Naoki Hirono, Masanori Uchikawa, Arisa Tanigawa, Takeru Fujii et autres

SUMMARY During embryonic development, cellular competence to respond to differentiation signals changes dynamically. Although the mechanisms underlying competence acquisition have been extensively studied, those underlying competence loss remain unclear. In preimplantation mouse embryos, Hippo signaling shifts from regulating trophectoderm (TE) fate specification …

gb, jp (code pays fourni par la source)

Opioidergic activation of the descending pain inhibitory system underlies placebo analgesia

Hiroyuki Neyama, Yuping Wu, Yuka Nakaya, Shigeki Kato et autres

Placebo analgesia is caused by inactive treatment, implicating endogenous brain function involvement. However, the neurobiological basis remains unclear. In this study, we found that μ-opioid signals in the medial prefrontal cortex (mPFC) activate the descending pain inhibitory system to initiate placebo analgesia …

jp (code pays fourni par la source)

C–C Motif chemokine receptor-2 blockade ameliorates pulmonary hypertension in rats and synergizes with a pulmonary vasodilator

Naoki Tsuboya, Hirofumi Sawada, Yoshihide Mitani, Hironori Oshita et autres

AIMS: We investigated whether the disruption of C-C motif chemokine receptor (CCR) 2 may attenuate the development of pulmonary arterial hypertension (PAH) in any rat models with the reversal of the associated pro-inflammatory state and vascular dysfunction, and synergize with a conventional …

jp, bg (code pays fourni par la source)

Loss of aquaporin-4 impairs cerebrospinal fluid solute clearance through cerebrospinal fluid drainage pathways

Daisuke Kato, Hiroyuki Kameda, Naoya Kinota, Takaaki Fujii et autres

The aquaporin-4 (AQP4) water channel is essential in neurofluid dynamics. AQP4 loss impairs solute exchange between the cerebrospinal fluid (CSF) and interstitial fluid (ISF). However, whether AQP4 expression affects solute clearance from the CSF space to the extracranial space remains unclear. This …

jp (code pays fourni par la source)

Humanized-Aquaporin-4-Expressing Rat Created by Gene-Editing Technology and Its Use to Clarify the Pathology of Neuromyelitis Optica Spectrum Disorder

Chihiro Namatame, Yoichiro Abe, Yoshiki Miyasaka, Yoshiki Takai et autres

Conventional rodent neuromyelitis optica spectrum disorder (NMOSD) models using patient-derived immunoglobulin G (IgG) are potentially affected by the differences between the human and rodent aquaporin-4 (AQP4) extracellular domains (ECDs). We hypothesized that the humanization of AQP4 ECDs would make the rodent model …

jp (code pays fourni par la source)

Age-related ciliopathy: Obesogenic shortening of melanocortin-4 receptor-bearing neuronal primary cilia

Manami Oya, Yoshiki Miyasaka, Yoshiko Nakamura, Miyako Tanaka et autres

Obesity is often associated with aging. However, the mechanism of age-related obesity is unknown. The melanocortin-4 receptor (MC4R) mediates leptin-melanocortin anti-obesity signaling in the hypothalamus. Here, we discovered that MC4R-bearing primary cilia of hypothalamic neurons progressively shorten with age in rats, correlating …

jp (code pays fourni par la source)

Protocol for highly selective transgene expression through the flip-excision switch system by using a unilateral spacer sequence in rodents

Natsuki Matsushita, Shigeki Kato, Kayo Nishizawa, Masateru Sugawara et autres

We present a protocol to induce Cre-dependent transgene expression in specific cell types in the rat brain, suppressing a leak expression in off-target cells, by using a flip-excision switch system with a unilateral spacer sequence. We describe steps for construction of transfer …

jp (code pays fourni par la source)

Hydrogen sulfide and polysulfides induce GABA/glutamate/d-serine release, facilitate hippocampal LTP, and regulate behavioral hyperactivity

Hiroki Furuie, Yuka Kimura, Tatsuhiro Akaishi, Misa Yamada et autres

Abstract Hydrogen sulfide (H2S) and polysulfides (H2Sn, n ≥ 2) are signaling molecules produced by 3-mercaptopyruvate sulfurtransferase (3MST) that play various physiological roles, including the induction of hippocampal long-term potentiation (LTP), a synaptic model of memory formation, by enhancing N-methyl-d-aspartate (NMDA) receptor …

jp (code pays fourni par la source)

Opioidergic activation of descending pain inhibitory system underlies placebo analgesia

Hiroyuki Neyama, Yuping Wu, Yuka Nakaya, Shigeki Kato et autres

Abstract Placebo analgesia is caused by inactive treatment, implicating endogenous brain function involvement. However, the underlying neurobiological mechanisms remain unclear. We found that μ-opioid signals in the medial prefrontal cortex (mPFC) activate the descending pain inhibitory system to initiate placebo analgesia in …

jp (code pays fourni par la source)

Highly selective transgene expression through the flip-excision switch system by using a unilateral spacer sequence

Natsuki Matsushita, Shigeki Kato, Kayo Nishizawa, Masateru Sugawara et autres

The flip-excision switch (FLEX) system with an adeno-associated viral (AAV) vector allows expression of transgenes in specific cell populations having Cre recombinase. A significant issue with this system is non-specific expression of transgenes in tissues after vector injection. We show here that …

jp (code pays fourni par la source)

Catecholaminergic cell type-specific expression of Cre recombinase in knock-in transgenic rats generated by the Combi-CRISPR technology

Natsuki Matsushita, Kayo Nishizawa, Shigeki Kato, Yoshio Iguchi et autres

jp (code pays fourni par la source)

A high-quality severe combined immunodeficiency (SCID) rat bioresource

Yoshiki Miyasaka, Jinxi Wang, Kosuke Hattori, Yuko Yamauchi et autres

Immunodeficient animals are valuable models for the engraftment of exogenous tissues; they are widely used in many fields, including the creation of humanized animal models, as well as regenerative medicine and oncology. Compared with mice, laboratory rats have a larger body size …

jp (code pays fourni par la source)