Tetsuya Kitaguchi

Green genetically encoded IP 3 biosensor for hierarchical analysis of its signaling network

Li Tian, Keisuke Yamashita, Zixu Feng, Takashi TSUBOI et autres

ABSTRACT Inositol 1,4,5-trisphosphate (IP 3 ) is a key second messenger that regulates diverse physiological processes. Visualization of IP 3 dynamics in living cells is therefore important for understanding its signaling processes. In this study, we developed genetically encoded green fluorescent IP …

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Harnessing the Power of SMART Single-Molecule Display for Enzyme Evolution: A Focus on Oxidase

Kalhari Munaweera, Nana Odake, Hannah Patricia Halim, Kakeru Ikeda et autres

For a rapid and cost-effective evolution of tailor-made enzymes, we established a high-throughput in vitro selection platform named SMART (Single-Molecule Assay on Ribonucleic acid by Translated product), integrating mRNA display, next-generation sequencing, and bioinformatics. SMART represents a versatile system where a module …

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qMaLioffG: a genetically encoded green fluorescence lifetime-based indicator enabling quantitative imaging of intracellular ATP

Satoshi Arai, Hideki Itoh, Cong Quang Vu, Loan Thi Ngoc Nguyen et autres

Genetically encoded indicators that can detect concentrations of metabolites and signalling molecules through fluorescence lifetime changes are gaining attention, because they expand the potential for quantitative imaging. These indicators offer advantages over conventional fluorescence intensity-based indicators by minimizing artifacts such as variations …

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Switchbody, an Antigen‐Responsive Enzyme Switch Based on Antibody and Its Working Principle (Adv. Sci. 44/2025)

Takanobu Yasuda, Yoshiyuki Ueno, Masahiko Taguchi, N. Tochio et autres

Antigen-Responsive Enzyme Switch In their Research Article (DOI: 10.1002/advs.202508422), Tetsuya Kitaguchi, Takanobu Yasuda, and co-workers develop an antigen-responsive enzyme switch “Switchbody” and elucidated its mechanism, in which antigen binding triggers the release of the trapped enzyme fragment, restoring activity through assembling with …

Correction: In situ FRET measurement of cellular tension using conventional confocal laser microscopy in newly established reporter mice expressing actinin tension sensor

Junfeng Wang, Eijiro MAEDA, Yuki Tsujimura, Takaya Abe et autres

In the original version of this Article Junfeng Wang and Eijiro Maeda were omitted as equally contributing authors. The Author Contributions section now reads:

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Switchbody, an Antigen‐Responsive Enzyme Switch Based on Antibody and Its Working Principle

Takanobu Yasuda, Yoshiyuki Ueno, Masahiko Taguchi, N. Tochio et autres

An enzyme switch, termed "Switchbody", is developed by fusing an antibody with a fragment of a split enzyme for the precise regulation of enzyme activity in response to an antigen. A luciferase-based Switchbody is engineered by fusing the NanoLuc luciferase fragment HiBiT …

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A Review on the Application of Biosensors for Monitoring Emerging Contaminants in the Water Environment

Yi Xiao, Zhe Du, Yuqian Li, Lijia Cao et autres

Due to the frequent occurrence and elevated concentrations of emerging contaminants (ECs) in water environments, as well as their high toxicity, these compounds have become a growing concern, threatening water safety, human health, and environmental health. Stricter regulations and routine monitoring are …

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Cerebral blood flow is modulated by astrocytic cAMP elevation independently of IP 3 R2-mediated Ca 2+ signaling in mice

Marta Vittani, Rasmus Herlo, Xiaowen Wang, Michala Daniela Bach Christensen et autres

Local neural activation drives regional increase of cerebral blood flow (CBF), in a phenomenon known as functional hyperemia. Astrocytes, which enwrap cerebral blood vessels and respond to neuronal activity through their G protein–coupled receptors (GPCRs), play a vital role in brain energy …

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N-Terminal Conjugation of a Near-Infrared Photosensitizer to an Antibody for Cancer Diagnosis and Treatment

Zhirou Qiu, Bo Zhu, Takanobu Yasuda, Hiroshi Ueda et autres

High Resolution Image Download MS PowerPoint Slide We developed immunoprobes by conjugating fluorophores to the N-terminal region of an antibody to detect cancer markers via fluorescence and destruct cancer cells via antigen-dependent photosensitization. To fabricate an immunoprobe for detection, peptides conjugated with …

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Cardiac dysfunction due to mitochondrial impairment assessed by human iPS cells caused by DNM1L mutations

Madori Osawa, Yasunori Fujita, Kazuki Kagami, Masataka Ito et autres

Abstract Background DNM1L encodes dynamin-related protein 1, which plays an important role in mitochondrial and peroxisomal division. The DNM1L mutation leads to cardiac dysfunction in patients and animal models. However, the mechanism of cardiac dysfunction caused by DNM1L mutation has not been …

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Detection of a large antigen through the masking and exposure of a fragment of split luciferase

Cheng Qian, Natsuki Shibukawa, Hiroshi Ueda, Takanobu Yasuda et autres

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SMART platform for enzyme engineering: A novel single-molecule display approach for evolution and functional studies of D-amino acid oxidase

Kalhari Munaweera, Nana Odake, Bo Zhu, Maurizio Camagna et autres

ABSTRACT This study introduces SMART ( S ingle M olecule A ssay on R ibonucleic Acid by T ranslated Product), an innovative in vitro platform integrating mRNA display, next-generation sequencing, and bioinformatics to address several key challenges in enzyme engineering. The characteristic …

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