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Poster session 3Cell growth, differentiation and stem cells - Heart511The role of the endocannabinoid system in modelling muscular dystrophy cardiac disease with induced pluripotent stem cells.512An emerging role of T lymphocytes in cardiac regenerative processes in heart failure due to dilated cardiomyopathy513Canonical wnt signaling reverses the ‘aged/senescent’ human endogenous cardiac stem cell phenotype514Hippo signalling modulates survival of human induced pluripotent stem cell-derived cardiomyocytes515Biocompatibility of mesenchymal stem cells with a spider silk matrix and its potential use as scaffold for cardiac tissue regeneration516A snapshot of genome-wide transcription in human induced pluripotent stem cell-derived hepatocyte-like cells (iPSC-HLCs)517Can NOS/sGC/cGK1 pathway trigger the differentiation and maturation of mouse embryonic stem cells (ESCs)?518Introduction of external Ik1 to human-induced pluripotent stem cell-derived cardiomyocytes via Ik1-expressing HEK29351

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16Institutions déclarées
7Pays d’affiliation déclarés

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Le résumé fourni par la source

Muscular Dystrophy (MD) is an umbrella term for genetic disorders affecting skeletal and cardiac muscle which arise due to abnormalities in the dystrophin gene.Underlying dystrophin defects cause metabolic and structural abnormalities in cardiomyocytes (CMs) which in turn become predisposed to ectopic cell death and fibro-fatty replacement.The Endogenous Cannabinoid System (ECS) is a lipid signalling network present in the cardiovascular system and comprises G-protein coupled receptors (CBR1 and CBR2), endogenous ligands (anandamide and 2-arachidonoylglycerol) and regulatory proteins (fatty acid amide hydrolase and monoacylglycerol lipase).The ECS has an emerging function in stem cell survival and differentiation, MD skeletal muscle pathology, and cardiovascular diseases in general.Induced Pluripotent Stem Cell (iPSC) technology permits the reprogramming of somatic cells (e.g.fibroblasts) into pluripotent stem cells, which can be differentiated into cells from all three germ layers including CMs.In the present study we provide evidence that the ECS is involved in somatic cell reprogramming.Specifically, the CBR1 antagonist AM251 prevented the formation of iPSC colonies (p≤0.05, vs. control conditions, Newman-Keuls multiple comparison test, n=3).CMs derived from MD patients' iPSCs (MD-CMs) displayed disease hallmarks such as lack of dystrophin expression, increased expression of Nup153 (a cardiomyopathy-associated protein; p=0.0009, vs. healthy CMs, Student's unpaired t test, n=3) and increased CM cell death (p≤0.0001, vs. healthy CMs, Student's unpaired t test, n=3).Furthermore, we also provide evidence that the ECS is present in iPSCs and becomes dysregulated in MD-CMs.Our results highlight the dual functionality of the ECS in cell reprogramming and MD cardiac pathology which is of interest to cardiac disease modelling and novel drug discovery.

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Le contrôle bibliographique ouvert

DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.

Titre Crossref
Poster session 3Cell growth, differentiation and stem cells - Heart511The role of the endocannabinoid system in modelling muscular dystrophy cardiac disease with induced pluripotent stem cells.512An emerging role of T lymphocytes in cardiac regenerative processes in heart failure due to dilated cardiomyopathy513Canonical wnt signaling reverses the ‘aged/senescent’ human endogenous cardiac stem cell phenotype514Hippo signalling modulates survival of human induced pluripotent stem cell-derived cardiomyocytes515Biocompatibility of mesenchymal stem cells with a spider silk matrix and its potential use as scaffold for cardiac tissue regeneration516A snapshot of genome-wide transcription in human induced pluripotent stem cell-derived hepatocyte-like cells (iPSC-HLCs)517Can NOS/sGC/cGK1 pathway trigger the differentiation and maturation of mouse embryonic stem cells (ESCs)?518Introduction of external Ik1 to human-induced pluripotent stem cell-derived cardiomyocytes via Ik1-expressing HEK29351
Date Crossref
01/07/2016
Éditeur
Oxford University Press (OUP)
Type
journal-article

Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.

Les institutions déclarées

Une affiliation ne permet pas de déduire la nationalité d’un auteur.

Les sujets associés

Tissue Engineering and Regenerative MedicineElectrospun Nanofibers in Biomedical ApplicationsPluripotent Stem Cells Research

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