Johannes Wedel

Multiomic Immune Profiling of Pediatric Transplant Recipients Identifies Cell States Associated with Vaccine Response

Johannes Wedel, Ying Tang, Bayan Alsairafi, Vicki Do et autres

KEY POINTS: Pediatric transplant recipients have oligoclonal expansion of CD8 + T cells and polyclonal expansion of CD4 + T cells versus healthy children. Analysis of vaccine responses revealed patients with intact T cell immunity but impaired humoral immunity. A subset of …

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Targeting AXL can effectively overcome c-Met-induced therapeutic resistance in renal cancer and promote tumor cell death through increased oxidative stress

Akash Sabarwal, Marc Machaalani, Laxminarayan Rawat, Johannes Wedel et autres

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Immunoevasion by Intragraft Semaphorin 3F Following Transplantation

Sayantan Bose, Masaaki Komatsu, V. Do, Diane R. Bielenberg et autres

Purpose: The Semaphorin3 family of molecules were originally discovered as neuronal guidance molecules and have recently been established to function in tumor-associated immunomodulation. However, little is known about their biology in alloimmunity and their role in allograft rejection is unexplored to date. …

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Effects of Targeting the Cullin RING E3 Ubiquitin Ligase Using MLN4924 on the Alloimmune Response

Bayan Alsairafi, C.L. Miller, O J.M., G. Benichou et autres

Purpose: We previously reported that DEPTOR is expressed in murine CD4+ T cells and that it is functional in Treg stability via its effects to augment Foxp3 expression and regulate cellular metabolism. MLN4924 (MLN) is a Cullin Ring E3 ligase inhibitor that …

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Adaptive Responses within the Graft Endothelial Cell as a Determinant of Long-Term Survival

Masaaki Komatsu, Viet Do, Sayantan Bose, Matthieu Boone et autres

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Neuropilin 2 Expression Pattern on Murine CD4+ T Cell Subsets

Johannes Wedel, Bayan Alsairafi, Masaaki Komatsu, David M. Briscoe

Heterogeneity in Activation and Immunomodulatory Responses in Heart, Kidney and Lung Endothelial Cells

Satyawan Jadhav, Viet Do, Alvin T. Kho, Sayantan Bose et autres

Purpose: Recent studies using single-cell RNA sequencing have revealed that there is considerable heterogeneity in endothelial cells (EC) phenotypes as well as notable differences in microvascular EC subpopulations across various organs. We wish to evaluate if organ-specific gene expression signatures in EC …

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Identification of Neuropilin 2 on a B Cell Subset with a Regulatory Phenotype

Nicolas Sailliet, Mai Komatsu, David M. Briscoe, Johannes Wedel

Purpose: In previous studies, we identified a select human B cell subset with Neuropilin 2 (NRP2) expression in a humanized SCID-beige mouse model of xenoactivation. We also found that NRP2 activity is required for long-term cardiac graft survival in a murine transplantation …

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Neuropilin-2 functions as a coinhibitory receptor to regulate antigen-induced inflammation and allograft rejection

Johannes Wedel, Nora Kochupurakkal, Sek Won Kong, Sayantan Bose et autres

Coinhibitory receptors function as central modulators of the immune response to resolve T effector activation and/or to sustain immune homeostasis. Here, using humanized SCID mice, we found that neuropilin-2 (NRP2) is inducible on late effector and exhausted subsets of human CD4+ T …

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Neuropilin-2 functions as a co-inhibitory receptor to regulate antigen-induced inflammation and allograft rejection

Johannes Wedel, Nora Kochupurakkal, Sek Won Kong, Sayantan Bose et autres

Abstract Co-inhibitory receptors function as central modulators of the immune response to resolve T effector activation and/or to sustain immune homeostasis. Here, using humanized SCID mice, we found that NRP2 is inducible on late effector and exhausted subsets of human CD4 + …

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Personalized Immune Profiling in Pediatric Transplant Recipients: Linking Atypical B Cells to Vaccine Response

Johannes Wedel, Ying Tang, Bayan Alsairafi, Vicki Do et autres

Abstract Immunosuppression in solid organ transplant recipients inhibits protective immune responsiveness to pathogens and vaccines. However, specific cell states that associate with failure to generate protective immunity are not known. Here, we perform scRNAseq and CyTOF analyses of PBMC from 12 pediatric …

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Immunomodulation by allograft endothelial cells

Sayantan Bose, Vicki Do, Chiara Testini, Suchita Suryakant Jadhav et autres

It is increasingly appreciated that the expression of immunoregulatory molecules within tumors have potential to shape a microenvironment that promotes local immunoevasion and immunoregulation. However, little is known about tissue-intrinsic immunomodulatory mechanisms following transplantation. We propose that differences in the phenotype of …

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