Sandip Patel

Scalable Production of a De Novo SARS-CoV-2 Antiviral Miniprotein in Escherichia coli

Jinhwan Shin, Eu-min Kim, Jun-Hong Jang, Seok-won Jee et autres

Background/Objectives: The rapid emergence of SARS-CoV-2 variants that evade neutralizing antibodies underscores the need for new antiviral biologics that integrate precise molecular design with scalable, cost-effective manufacturing. Computationally designed miniproteins targeting the spike protein’s receptor-binding domain (RBD) offer a promising alternative to …

kr, us (code pays fourni par la source)

TPC2-mediated lysosomal Ca2+ release puts the brakes on store-operated Ca2+ entry

Qianru Mu, Sandip Patel, David Yule, Siddhi Shetty

gb, us (code pays fourni par la source)

Allosteric switching of Ca2+ permeability in the lysosomal ion channel TPC2 underpins biased agonist activation

Qianru Mu, Ryo� Saito, Franceska Ozola, Dawid Jaślan et autres

Abstract Ion channels possess selectivity filters that are hardwired to ensure the selective passage of ions. Lysosomal two-pore channels are unusual as they are able to switch their cation selectivity in an agonist-specific manner, allowing differential control of organellar activity. TPC2 is …

gb, jp, de, us (code pays fourni par la source)

Machine Learning-Enabled Raman Spectroscopy for Process Analytical Technology and Real-Time Release Testing in Bioprocess Manufacturing: A Comparative Predictive Modeling Study

Viral Patel, Sandip Patel

Abstract Analytical technologies that can provide quick, precise, and continuous information regarding process performance are necessary for the development of biopharmaceutical manufacturing. Conventional bioprocess monitoring is largely dependent on laboratory-based data and offline sampling, which can restrict process management and cause delays …

us (code pays fourni par la source)

Unravelling the resistome of carbapenem-resistant E. coli from bovine mastitis via whole-genome sequencing

Sandip Patel, Janki Panchal, Arun C. Patel, Harshad Chauhan et autres

in (code pays fourni par la source)

Scalable Production of a De Novo SARS-CoV-2 Antiviral miniprotein in E scherichia coli

Jinhwan Shin, E Sle Kim, Jun-Hong Jang, Seok-won Jee et autres

Abstract The rapid emergence of SARS-CoV-2 variants that evade neutralizing antibodies underscores the need for next-generation antiviral biologics that combine molecular precision with scalable, cost-effective manufacturing. Computationally designed miniproteins targeting the receptor-binding domain (RBD) of the spike protein offer a compelling alternative …

us (code pays fourni par la source)

Conformational footprints of agonism: differences of inositol 1,4,5-trisphosphate (IP3) and adenophostin A (AdA) on IP3 receptor’s N-terminal dynamics

Yu Zhu, Sandip Patel

Abstract Inositol 1,4,5-trisphosphate receptor (IP3R) represents a major family of intracellular Ca2+-release channels. Distal to its pore-forming region lies its cytoplasmic N-terminus (NT) that harbours the agonist-binding pocket. Although inositol 1,4,5-trisphosphate (IP3) is the endogenous agonist, the fungal metabolite adenophostin A (AdA) …

Time-Temperature-Aware Multi-Output Machine Learning Framework for Predicting Viscosity, Aggregation, Stability, and Bioactivity Decay in High-Concentration Monoclonal Antibody Formulations

Viral Patel, Sandip Patel

us (code pays fourni par la source)

Allosteric switching of Ca2+ permeability in the lysosomal ion channel TPC2 underpins biased agonist activation

Qianru Mu, Ryo� Saito, Franceska Ozola, Dawid Jaślan et autres

Ion channels possess selectivity filters that are hardwired to ensure the selective passage of ions. Lysosomal two-pore channels are unusual as they are able to switch their cation selectivity in an agonist-specific manner, allowing differential control of organellar activity. TPC2 is permeable …

Direct measurements of luminal Ca2+ with endo-lysosomal GFP-aequorin reveal functional IP3 receptors

Belén Calvo, Patricia Torres-vidal, Alba Delrio-Lorenzo, Carla Rodríguez-Crespo et autres

Endo-lysosomes are considered acidic Ca2+ stores, but direct measurements of luminal Ca2+ within them are limited. Here, we report that the Ca2+-sensitive luminescent protein aequorin does not reconstitute with its cofactor at highly acidic pH but that a significant fraction of the …

es, us, de, gb (code pays fourni par la source)

Alternative Splicing Directs PMCA2 to Lysosomes and is Linked to Neurodegeneration

María E. Fernández‐Suárez, Robert K. Bush, Jonathan Brenton, Gustavo J.S. Pereira et autres

Abstract Plasma membrane calcium ATPases (PMCAs) are believed to function exclusively at the plasma membrane where they expel calcium from the cytosol. We have unexpectedly identified a splice variant-dependent localisation of the PMCA isoform PMCA2 to the lysosome, where it forms an …

gb, us, br, es (code pays fourni par la source)

OR25-07 A Novel and Severe Multisystem Metabolic Disease due to Homozygous AIP Variants

Márta Korbonits, Xian Wang, Sayka Barry, Oniz Suleyman et autres

Abstract Disclosure: M. Korbonits: Novo Nordisk, Ipsen, Pfizer, Inc.. X. Wang: None. S. Barry: None. O. Suleyman: None. D. Stefano: None. N. Uddin: None. C.L. Hall: None. P. Laura: None. P. Chapple: None. H. Sian: None. V. Morales: None. K. Bianchi: None. …

gb, ru, is, us (code pays fourni par la source)