Eugen Brailoiu

Allosteric switching of Ca2+ permeability in the lysosomal ion channel TPC2 underpins biased agonist activation

Qianru Mu, Ryo� Saito, Franceska Ozola, Dawid Jaślan et autres

Abstract Ion channels possess selectivity filters that are hardwired to ensure the selective passage of ions. Lysosomal two-pore channels are unusual as they are able to switch their cation selectivity in an agonist-specific manner, allowing differential control of organellar activity. TPC2 is …

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Allosteric switching of Ca2+ permeability in the lysosomal ion channel TPC2 underpins biased agonist activation

Qianru Mu, Ryo� Saito, Franceska Ozola, Dawid Jaślan et autres

Ion channels possess selectivity filters that are hardwired to ensure the selective passage of ions. Lysosomal two-pore channels are unusual as they are able to switch their cation selectivity in an agonist-specific manner, allowing differential control of organellar activity. TPC2 is permeable …

Choline—An Essential Nutrient with Health Benefits and a Signaling Molecule

Jitendra D. Belani, Hailey N. Wittorf, Eugen Brailoiu, G. Cristina Brailoiu

Choline has been recognized as an essential nutrient involved in various physiological functions critical to human health. Adequate daily intake of choline has been established by the US National Academy of Medicine in 1998, considering choline requirements for different ages, sex differences …

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Dopamine internalization via Uptake2 and stimulation of intracellular D5-receptor-dependent calcium mobilization and CDP-diacylglycerol signaling

Wenfei Kang, Arlette Deukam Siewe, Chizurum C. Oluigbo, Mercy O. Arijesudade et autres

Dopamine stimulates CDP-diacylglycerol biosynthesis through D1-like receptors, particularly the D5 subtype most of which is intracellularly localized. CDP-diacylglycerol regulates phosphatidylinositol-4,5-bisphosphate-dependent signaling cascades by serving as obligatory substrate for phosphatidylinositol biosynthesis. Here, we used acute and organotypic brain tissues and cultured cells to …

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Neuronal dopamine internalization and intracellular stimulation of D5-receptor-dependent CDP-diacylglycerol biosynthesis for phosphoinositide signaling

Ashiwel S. Undieh, Chizurum C. Oluigbo, Mercy O. Arijesudade, Eugen Brailoiu et autres

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Modulation of the Blood–Brain Barrier by Sigma-1R Activation

Eugen Brailoiu, Jeffrey L. Barr, Hailey N. Wittorf, Saadet Inan et autres

Sigma non-opioid intracellular receptor 1 (Sigma-1R) is an intracellular chaperone protein residing on the endoplasmic reticulum at the mitochondrial-associated membrane (MAM) region. Sigma-1R is abundant in the brain and is involved in several physiological processes as well as in various disease states. …

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Two-pore channel-2 and inositol trisphosphate receptors coordinate Ca2+ signals between lysosomes and the endoplasmic reticulum

Yu Yuan, Vikas Arige, Ryo� Saito, Qianru Mu et autres

Lysosomes and the endoplasmic reticulum (ER) are Ca 2+ stores mobilized by the second messengers NAADP and IP 3 , respectively. Here, we establish Ca 2+ signals between the two sources as fundamental building blocks that couple local release to global changes …

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Progesterone receptor membrane component 1 facilitates Ca2+ signal amplification between endosomes and the endoplasmic reticulum

Gihan S. Gunaratne, Sushil Kumar, Yaping Lin-Moshier, James T. Slama et autres

Membrane contact sites (MCSs) between endosomes and the endoplasmic reticulum (ER) are thought to act as specialized trigger zones for Ca 2+ signaling, where local Ca 2+ released via endolysosomal ion channels is amplified by ER Ca 2+ -sensitive Ca 2+ channels …

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Convergent activation of Ca 2+ permeability in two-pore channel 2 through distinct molecular routes

Ryo� Saito, Qianru Mu, Yu Yuan, Marcos Rubio‐Alarcón et autres

TPC2 is a pathophysiologically relevant lysosomal ion channel that is activated directly by the phosphoinositide PI(3,5)P 2 and indirectly by the calcium ion (Ca 2+ )–mobilizing molecule NAADP through accessory proteins that associate with the channel. TPC2 toggles between PI(3,5)P 2 -induced, …

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Convergent activation of two-pore channels mediated by the NAADP-binding proteins JPT2 and LSM12

Gihan S. Gunaratne, Eugen Brailoiu, Sushil Kumar, Yu Yuan et autres

The second messenger nicotinic acid adenine dinucleotide phosphate (NAADP) evokes calcium ion (Ca 2+ ) release from endosomes and lysosomes by activating two-pore channels (TPCs) on these organelles. Rather than directly binding to TPCs, NAADP associates with proteins that indirectly confer NAADP …

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In Memoriam of Professor Mary E. Abood

Eugen Brailoiu, Linda Console‐Bram, Pingwei Zhao

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Supplementary Methods from The G Protein–Coupled Receptor GPR30 Inhibits Proliferation of Estrogen Receptor–Positive Breast Cancer Cells

Eric A. Ariazi, Eugen Brailoiu, Smitha Yerrum, Heather A. Shupp et autres

Supplementary Methods from The G Protein–Coupled Receptor GPR30 Inhibits Proliferation of Estrogen Receptor–Positive Breast Cancer Cells