Mohammad S. Khalifa

hInGeTox: a human-based in vitro platform to evaluate lentivirus/host interactions that contribute to genotoxicity

Saqlain Suleman, Sharmin Alhaque, Andrew Y. Guo, Huairen Zhang et autres

Abstract Lentivirus vectors are effective for treatment of genetic disease. However, safety associated with vector related genotoxicity is of concern and currently available models are not reliably predictive of safety in humans. We have developed h InGeTox as the first human in …

gb, de (code pays fourni par la source)

Transcriptomic Profiling of iPS Cell-Derived Hepatocyte-like Cells Reveals Their Close Similarity to Primary Liver Hepatocytes

Saqlain Suleman, Sharmin Alhaque, Andrew Y. Guo, Aaron Zhang et autres

Human-induced pluripotent stem cell (iPSC)-derived hepatocyte-like cells (HLCs) have been shown to be useful for the development of cell-based regenerative strategies and for modelling drug discovery. However, stem cell-derived HLCs are not identical in nature to primary human hepatocytes (PHHs), which could …

gb (code pays fourni par la source)

Analysis of HIV-1-Based Lentiviral Vector Particle Composition by PacBio Long-Read Nucleic Acid Sequencing

Saqlain Suleman, Mohammad S. Khalifa, Serena Fawaz, Sharmin Alhaque et autres

Lentivirus (LV) vectors offer permanent delivery of therapeutic genes to the host through an RNA intermediate genome. They are one of the most commonly used vectors for clinical gene therapy of inherited disorders such as immune deficiencies and cancer immunotherapy. One of …

gb (code pays fourni par la source)

hInGeTox: A human-based in vitro platform to evaluate lentivirus contribution to genotoxicity

Michael Themis, Saqlain Suleman, Sharmin Al Haque, Andrew Y. Guo et autres

gb, de, cz (code pays fourni par la source)

Wastewater monitoring for detection of public health markers during the COVID-19 pandemic: Near-source monitoring of schools in England over an academic year

Francis Hassard, Milan Vu, Shadi Rahimzadeh, Víctor Castro-Gutiérrez et autres

BACKGROUND: Schools are high-risk settings for infectious disease transmission. Wastewater monitoring for infectious diseases has been used to identify and mitigate outbreaks in many near-source settings during the COVID-19 pandemic, including universities and hospitals but less is known about the technology when …

Afrique du Sud, gb, cr (code pays fourni par la source)

Evaluation of variant calling algorithms for wastewater-based epidemiology using mixed populations of SARS-CoV-2 variants in synthetic and wastewater samples

Irene Bassano, Vinoy K. Ramachandran, Mohammad S. Khalifa, Chris J. Lilley et autres

Wastewater-based epidemiology has been used extensively throughout the COVID-19 (coronavirus disease 19) pandemic to detect and monitor the spread and prevalence of SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) and its variants. It has proven an excellent, complementary tool to clinical sequencing, …

gb (code pays fourni par la source)

Rapid Assessment of SARS-CoV-2 Variant-Associated Mutations in Wastewater Using Real-Time RT-PCR

Kata Farkas, Cameron Pellett, Rachel J. Williams, Natasha Alex-Sanders et autres

With the global spread of COVID-19, it is essential to identify emerging variants which may be more harmful or able to escape vaccines rapidly. To date, the gold standard to assess variants circulating in communities has been the sequencing of the S …

gb, au (code pays fourni par la source)

Comparison of multiple whole-genome and Spike -only sequencing protocols for estimating variant frequencies via wastewater-based epidemiology

Lucy A. Winder, Paul J. Parsons, Gavin J. Horsburgh, Kathryn H. Maher et autres

Abstract Sequencing of SARS-CoV-2 in wastewater provides a key opportunity to monitor the prevalence of variants spatiotemporally, potentially facilitating their detection simultaneously with, or even prior to, observation through clinical testing. However, there are multiple sequencing methodologies available. This study aimed to …

gb (code pays fourni par la source)

City-wide wastewater genomic surveillance through the successive emergence of SARS-CoV-2 Alpha and Delta variants

Franziska S. Brunner, Mathew Robert Brown, Irene Bassano, Hubert Denise et autres

Genomic surveillance of SARS-CoV-2 has provided a critical evidence base for public health decisions throughout the pandemic. Sequencing data from clinical cases has helped to understand disease transmission and the spread of novel variants. Genomic wastewater surveillance can offer important, complementary information …

gb, au (code pays fourni par la source)

Evaluation of variant calling algorithms for wastewater-based epidemiology using mixed populations of SARS-CoV-2 variants in synthetic and wastewater samples

Irene Bassano, Vinoy K. Ramachandran, Mohammad S. Khalifa, Chris J. Lilley et autres

Abstract Wastewater-based epidemiology (WBE) has been used extensively throughout the COVID-19 pandemic to detect and monitor the spread and prevalence of SARS-CoV-2 and its variants. It has proven an excellent, complementary tool to clinical sequencing, supporting the insights gained and helping to …

gb (code pays fourni par la source)

HIV- 1 lentivirus tethering to the genome is associated with transcription factor binding sites found in genes that favour virus survival

Saqlain Suleman, Annette M. Payne, Johnathan Bowden, Sharmin Al Haque et autres

Lentiviral vectors (LV) are attractive for permanent and effective gene therapy. However, integration into the host genome can cause insertional mutagenesis highlighting the importance of understanding of LV integration. Insertion site (IS) tethering is believed to involve cellular proteins such as PSIP1/LEDGF/p75, …

gb, de, us, Afrique du Sud (code pays fourni par la source)

City-wide wastewater genomic surveillance through the successive emergence of SARS-CoV-2 Alpha and Delta variants

Franziska S. Brunner, Mathew Robert Brown, Irene Bassano, Hubert Denise et autres

Abstract Genomic surveillance of SARS-CoV-2 has been essential to provide an evidence base for public health decisions throughout the SARS-CoV-2 pandemic. Sequencing data from clinical cases has provided data crucial to understanding disease transmission and the detection, surveillance, and containment of outbreaks …

gb, au (code pays fourni par la source)