Mami Matsuda‐Lennikov

Thymic cortical epithelial Psmb11 -encoded β5t in mouse and human

Meiting Yang, Akihide Shimizu, Mami Matsuda‐Lennikov, J LI et autres

Psmb11-encoded β5t is a proteolytic subunit of thymoproteasomes expressed in cortical thymic epithelial cells (cTECs). β5t-containing thymoproteasomes are essential for the thymic generation of CD8-lineage cytotoxic T cells. Here, we outline the current understanding of the mechanisms underlying thymoproteasome-dependent CD8 T cell …

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Proteasome alteration between epithelial and hematopoietic cells facilitates positive selection of CD8 T cells

Mami Matsuda‐Lennikov, Jamie-Jean De La Torre, Todd Snow, Jacqueline Battaile et autres

The thymoproteasome, a proteolytic complex uniquely expressed in cortical thymic epithelial cells (cTECs), governs the positive selection of CD8 T cells. It has been hypothesized that the thymoproteasome promotes CD8 T cell development by forging a sequential switch in proteasome species between …

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Epithelial chemokine CCL25 integrates T cell development and intestinal homeostasis

J LI, Meiting Yang, Mami Matsuda‐Lennikov, Felix Kalle-Youngoue et autres

The chemokine receptor CCR9 regulates thymus colonization by T progenitors and intestinal accumulation of TCRγδ+ intraepithelial lymphocytes (IELs). CCR9-ligand chemokine CCL25 is expressed predominantly in the thymus and the small intestine. By engineering tissue-specific CCL25-deficient mice, we demonstrate that CCL25 locally produced …

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Integrated Workflow for Chromatin Architecture Analysis in Rare Thymic Cells Using Focused Ion Beam Scanning Electron Microscopy v1

Zulfeqhar Ali Syed, Ye Sun, Mami Matsuda‐Lennikov, Yousuke Takahama et autres

The thymus, a primary lymphoid organ, plays a critical role in T-cell development and selection including the establishment of central tolerance. To investigate chromatin organisation in cells that regulate thymocyte differentiation, we developed an optimised workflow to quantify heterochromatin-to-euchromatin ratios among different …

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Preparation of Single-cell Suspension of Mouse Thymic Epithelial Cells and Staining of Intracellular Molecules for Flow Cytometric Analysis

Meiting Yang, Jie Li, Mami Matsuda‐Lennikov, Assiatu Crossman et autres

Thymic epithelial cells (TECs) play an essential role in promoting the development and repertoire selection of T cells. Cortical TECs (cTECs) in the thymic cortex induce early T cell development and positive selection of cortical thymocytes. In contrast, medullary TECs (mTECs) in …

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Functionally diverse thymic medullary epithelial cells interplay to direct central tolerance

Ushio Aya, Mami Matsuda‐Lennikov, Felix Kalle-Youngoue, Akihide Shimizu et autres

Medullary thymic epithelial cells (mTECs) are essential for the establishment of self-tolerance in T cells. Promiscuous gene expression by a subpopulation of mTECs regulated by the nuclear protein Aire contributes to the display of self-genomic products to newly generated T cells. Recent …

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Large-Scale Isolation of Mouse Thymic Epithelial Cells

Izumi Ohigashi, Mami Matsuda‐Lennikov, Yousuke Takahama

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Tissue-specific proteasomes in generation of MHC class I peptides and CD8+ T cells

Mami Matsuda‐Lennikov, Izumi Ohigashi, Yousuke Takahama

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Peptides for T cell selection in the thymus

Izumi Ohigashi, Mami Matsuda‐Lennikov, Yousuke Takahama

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Defective glycosylation and multisystem abnormalities characterize the primary immunodeficiency XMEN disease

Juan C. Ravell, Mami Matsuda‐Lennikov, Samuel D. Chauvin, Juan Zou et autres

X-linked immunodeficiency with magnesium defect, EBV infection, and neoplasia (XMEN) disease are caused by deficiency of the magnesium transporter 1 (MAGT1) gene. We studied 23 patients with XMEN, 8 of whom were EBV naive. We observed lymphadenopathy (LAD), cytopenias, liver disease, cavum …

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Magnesium transporter 1 (MAGT1) deficiency causes selective defects in N-linked glycosylation and expression of immune-response genes

Mami Matsuda‐Lennikov, Matthew Biancalana, Juan Zou, Juan C. Ravell et autres

Magnesium transporter 1 (MAGT1) critically mediates magnesium homeostasis in eukaryotes and is highly-conserved across different evolutionary branches. In humans, loss–of–function mutations in the MAGT1 gene cause X-linked magnesium deficiency with Epstein-Barr virus (EBV) infection and neoplasia (XMEN), a disease that has a …

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Mg2+ regulation of kinase signaling and immune function

Chryssa Kanellopoulou, Alex B. George, Evan Masutani, Jennifer Leigh Cannons et autres

Mg2+ is required at micromolar concentrations as a cofactor for ATP, enzymatic reactions, and other biological processes. We show that decreased extracellular Mg2+ reduced intracellular Mg2+ levels and impaired the Ca2+ flux, activation marker up-regulation, and proliferation after T cell receptor (TCR) …

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