Accès ouvert
2025
article
OpenAlex
Rebeca de Paiva Fróes Rocha, Marina Alves Fontoura, Fabrício Fredo Naciuk, Leonardo Camilo de Oliveira et autres
Usutu virus (USUV) is an emerging arbovirus recently associated with outbreaks in Western Europe. Although USUV is typically associated with asymptomatic or nonspecific febrile disease, the occurrence of severe neuroinvasive forms of disease has raised concern. There is currently no antiviral treatment …
br, us
(code pays fourni par la source)
Accès ouvert
2025
article
OpenAlex
Rebeca de Paiva Fróes Rocha, Ilhan Tomris, Charles A. Bowman, Eugene S. Stevens et autres
The quest for a universal influenza vaccine holds great promise for mitigating the global burden of influenza-related morbidity and mortality. However, challenges persist in identifying conserved epitopes capable of eliciting robust and durable immune responses. In this study, we explore the influence …
us, br, nl
(code pays fourni par la source)
Accès ouvert
2025
article
OpenAlex
Leigh M. Sewall, Rebeca de Paiva Fróes Rocha, Grace Gibson, Michelle Louie et autres
Understanding the mechanistic interplay between antibodies and invading pathogens is essential for vaccine development. Current methods are labour and time intensive and limited by sample preparation bottlenecks. Here we present microfluidic electron microscopy-based polyclonal epitope mapping (mEM), which combines microfluidics with single-particle …
us
(code pays fourni par la source)
Accès ouvert
2025
article
OpenAlex
Emily Slowikowski, C Willems, Robertha Mariana Rodrigues Lemes, Sara Schuermans et autres
Usutu virus (USUV) is an emerging neurotropic flavivirus capable of causing encephalitis in humans. Here, our main goal was to characterize the innate immune response in the brain during USUV encephalitis and to identify strategies to control disease severity. Using an immunocompetent …
be, br
(code pays fourni par la source)
Accès ouvert
2025
article
OpenAlex
Rebeca de Paiva Fróes Rocha, Laís D. Coimbra, Marina Alves Fontoura, Guilherme Ribeiro et autres
ABSTRACT Usutu virus (USUV) is an arbovirus and has emerged as a potential cause of encephalitis in humans and other vertebrates. The increasing detection of USUV in mosquitoes and birds across Africa and Central Europe, along with the lack of specific treatments …
br, us, be
(code pays fourni par la source)
Accès ouvert
2024
preprint
OpenAlex
Emily Slowikowski, C Willems, Robertha Mariana Rodrigues Lemes, Sara Schuermans et autres
ABSTRACT Usutu virus (USUV) is an emerging neurotropic flavivirus capable of causing encephalitis in humans. Here, our main goal was to characterize the innate immune response in the brain during USUV encephalitis and to identify strategies to control disease severity. Using an …
be, pl, br, fr
(code pays fourni par la source)
Accès ouvert
2024
article
OpenAlex
Sila Toksoz Ataca, Maya Sangesland, Rebeca de Paiva Fróes Rocha, Alba Torrents de la Peña et autres
Antibodies targeting epitopes through germline-encoded motifs can be found in different individuals. While these public antibodies are often beneficial, they also pose hurdles for subdominant antibodies to emerge. Here, we use transgenic mice that reproduce the human IGHV1-69 ∗ 01 germline-encoded antibody …
us
(code pays fourni par la source)
Accès ouvert
2024
preprint
OpenAlex
Rebeca de Paiva Fróes Rocha, Ilhan Tomris, Charles A. Bowman, Emma Stevens et autres
Summary The quest for a universal influenza vaccine holds great promise for mitigating the global burden of influenza-related morbidity and mortality. However, challenges persist in identifying conserved epitopes capable of inducing protection. In this study, we explore the influence of glycan evolution …
us, br, nl
(code pays fourni par la source)
Accès ouvert
2024
article
OpenAlex
Iván del Moral-Sánchez, Edmund G. Wee, Yuejiao Xian, Wen-Hsin Lee et autres
Recombinant native-like HIV-1 envelope glycoprotein (Env) trimers are used in candidate vaccines aimed at inducing broadly neutralizing antibodies. While state-of-the-art SOSIP or single-chain Env designs can be expressed as native-like trimers, undesired monomers, dimers and malformed trimers that elicit non-neutralizing antibodies are …
nl, gb, us, in, jp, ca
(code pays fourni par la source)
Accès ouvert
2024
article
OpenAlex
Ilhan Tomris, Roosmarijn van der Woude, Rebeca de Paiva Fróes Rocha, Alba Torrents de la Peña et autres
Enveloped viruses carry one or multiple proteins with receptor-binding functionalities. Functional receptors can be glycans, proteinaceous, or both; therefore, recombinant protein approaches are instrumental in attaining new insights regarding viral envelope protein receptor-binding properties. Visualizing and measuring receptor binding typically entails antibody …
nl, us
(code pays fourni par la source)
Accès ouvert
2023
preprint
OpenAlex
Iván del Moral-Sánchez, Edmund G. Wee, Yuejiao Xian, Wen-Hsin Lee et autres
Abstract Recombinant native-like HIV-1 envelope glycoprotein (Env) trimers are used in candidate vaccines aimed at inducing broadly neutralizing antibodies. While state-of-the-art SOSIP or single-chain Env designs can be expressed as native-like trimers, undesired monomers, dimers and malformed trimers that elicit non-neutralizing antibodies …
nl, gb, us, in, jp
(code pays fourni par la source)
Accès ouvert
2023
article
OpenAlex
Alba Torrents de la Peña, Leigh M. Sewall, Rebeca de Paiva Fróes Rocha, Abigail M. Jackson et autres
Understanding antibody-antigen interactions in a polyclonal immune response in humans and animal models is critical for rational vaccine design. Current approaches typically characterize antibodies that are functionally relevant or highly abundant. Here, we use photo-cross-linking and single-particle electron microscopy to increase antibody …
us
(code pays fourni par la source)