Phumrapee Boonklang

Spatial lung niches shape Pseudomonas aeruginosa persistence

Cecilia Kyanya, Duy Pham, Nana-Jane Chipampe, Phumrapee Boonklang et autres

Summary Chronic Pseudomonas aeruginosa lung infection is a major cause of morbidity and mortality in people with pre-existing lung disease. Once established, infection is rarely eradicated and often persists despite prolonged antimicrobial therapy, but the underlying mechanisms remain poorly understood. To define …

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CRISPR-based environmental detection of Burkholderia pseudomallei identifies sanitation gaps and melioidosis risk in northeast Thailand

Sukritpong Pakdeerat, Chalita Chomkatekaew, Phumrapee Boonklang, Raiwin Mothong et autres

Environmental exposure to Burkholderia pseudomallei, the causative agent of melioidosis, remains poorly characterised due to the low sensitivity of conventional detection methods. Here, we develop CRISPR-BEEPs, a sensitive and resource-efficient CRISPR-based assay, and evaluate its performance against conventional culture-based plate inspection using …

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CRISPR-based environmental detection of Burkholderia pseudomallei identifies sanitation gaps and melioidosis risk in northeast Thailand.

Sukritpong Pakdeerat, Chalita Chomkatekaew, Phumrapee Boonklang, Raiwin Mothong et autres

Environmental exposure to Burkholderia pseudomallei, the causative agent of melioidosis, remains poorly characterised due to the low sensitivity of conventional detection methods. Here, we develop CRISPR-BEEPs, a sensitive and resource-efficient CRISPR-based assay, and evaluate its performance against conventional culture-based plate inspection using …

Metformin use is associated with lower mortality from bacterial sepsis and improved immunocompetence in Thai diabetes patients with acute melioidosis

Barbara Kronsteiner, Clement Twumasi, Priyanka Abraham, Suchintana Chumseng et autres

ABSTRACT Background Diabetes mellitus (DM) is a major risk factor for acquiring infections. Metformin, the first-line treatment for type 2 DM, is associated with beneficial outcome from various infectious diseases. The neglected tropical disease melioidosis has up to 50% in-hospital mortality rate …

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CRISPR-based environmental detection of Burkholderia pseudomallei identifies sanitation gaps and melioidosis risk in northeast Thailand

Sukritpong Pakdeerat, Chalita Chomkatekaew, Phumrapee Boonklang, Arin Wongprommoon et autres

Abstract Environmental exposure to Burkholderia pseudomallei , the causative agent of melioidosis, remains poorly characterised due to the limited sensitivity of conventional detection methods. This hinders accurate risk mapping and delays public health responses. Here, we developed CRISPR-BEEPs – a sensitive, equipment-light …

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Benchmarking CRISPR-BP34 for point-of-care melioidosis detection in low-income and middle-income countries: a molecular diagnostics study

Sukripong Pakdeerat, Phumrapee Boonklang, Kesorn Angchagun, Chalita Chomkatekaew et autres

BACKGROUND: Melioidosis is a neglected but often fatal tropical disease. The disease has broad clinical manifestations, which makes diagnosis challenging and time consuming. To improve diagnosis, we aimed to evaluate the performance of the CRISPR-Cas12a system (CRISPR-BP34) to detect Burkholderia pseudomallei DNA …

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BurkHostGEN: a study protocol for evaluating variations in the Burkholderia pseudomallei and host genomes associated with melioidosis infection

Kesorn Angchagun, Phumrapee Boonklang, Chalita Chomkatekaew, Sukritpong Pakdeerat et autres

Background Melioidosis is a frequently fatal disease caused by an environmental bacterium Burkholderia pseudomallei . The disease is prevalent in northeast Thailand, particularly among rice field farmers who are at risk of bacterial exposure through contact with contaminated soil and water. However, …

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BurkHostGEN: a study protocol for evaluating variations in the Burkholderia pseudomallei and host genomes associated with melioidosis infection

Kesorn Angchagun, Phumrapee Boonklang, Chalita Chomkatekaew, Sukritpong Pakdeerat et autres

Background: Melioidosis is a frequently fatal disease caused by an environmental bacterium Burkholderia pseudomallei . The disease is prevalent in northeast Thailand, particularly among rice field farmers who are at risk of bacterial exposure through contact with contaminated soil and water. However, …

Benchmarking CRISPR-BP34 for point-of-care melioidosis detection in LMIC: a molecular diagnostics study

Sukripong Pakdeerat, Phumrapee Boonklang, Kesorn Angchagun, Chalita Chomkatekaew et autres

Summary Background Melioidosis is a grossly neglected but often-fatal tropical disease. The disease is named “a great mimicker” after its broad clinical manifestations, which makes disease diagnosis challenging and time-consuming. To improve diagnosis, we developed and evaluated the performance of the CRISPR-Cas12a …

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Highly specific and sensitive detection of Burkholderia pseudomallei genomic DNA by CRISPR-Cas12a

Somsakul Pop Wongpalee, Hathairat Thananchai, Claire Chewapreecha, Henrik B. Roslund et autres

Detection of Burkholderia pseudomallei, a causative bacterium for melioidosis, remains a challenging undertaking due to long assay time, laboratory requirements, and the lack of specificity and sensitivity of many current assays. In this study, we are presenting a novel method that circumvents …

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Corrigendum: An Evolutionary Arms Race Between Burkholderia pseudomallei and Host Immune System: What Do We Know?

Chalita Chomkatekaew, Phumrapee Boonklang, Apiwat Sangphukieo, Claire Chewapreecha

Corrigendum: An Evolutionary Arms Race Between Burkholderia pseudomallei and Host Immune System: What Do We Know?Name of all authors as they appear in the published original article Chalita Chomkatekaew, Phumrapee Boonklang, Apiwat Sangphukieo and Claire ChewapreechaAffiliations of all authors as they appear …

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Co-evolutionary Signals IdentifyBurkholderia pseudomalleiSurvival Strategies in a Hostile Environment

Claire Chewapreecha, Johan Pensar, Supaksorn Chattagul, Maiju Pesonen et autres

The soil bacterium Burkholderia pseudomallei is the causative agent of melioidosis and a significant cause of human morbidity and mortality in many tropical and subtropical countries. The species notoriously survives harsh environmental conditions but the genetic architecture for these adaptations remains unclear. …

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