Chalita Chomkatekaew

CRISPR-based environmental detection of Burkholderia pseudomallei identifies sanitation gaps and melioidosis risk in northeast Thailand

Sukritpong Pakdeerat, Chalita Chomkatekaew, Phumrapee Boonklang, Raiwin Mothong et autres

Environmental exposure to Burkholderia pseudomallei, the causative agent of melioidosis, remains poorly characterised due to the low sensitivity of conventional detection methods. Here, we develop CRISPR-BEEPs, a sensitive and resource-efficient CRISPR-based assay, and evaluate its performance against conventional culture-based plate inspection using …

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CRISPR-based environmental detection of Burkholderia pseudomallei identifies sanitation gaps and melioidosis risk in northeast Thailand.

Sukritpong Pakdeerat, Chalita Chomkatekaew, Phumrapee Boonklang, Raiwin Mothong et autres

Environmental exposure to Burkholderia pseudomallei, the causative agent of melioidosis, remains poorly characterised due to the low sensitivity of conventional detection methods. Here, we develop CRISPR-BEEPs, a sensitive and resource-efficient CRISPR-based assay, and evaluate its performance against conventional culture-based plate inspection using …

A 28-year evolution experiment on Burkholderia pseudomallei survival in nutrient-depleted sterile water

Chalita Chomkatekaew, Premjit Amornchai, Sayan Langlah, Janjira Thaipadungpanit et autres

Abstract Environmental persistence allows opportunistic pathogens to survive a range of harsh conditions, increasing the likelihood of eventual infection. Burkholderia pseudomallei , the causative agent of melioidosis, can endure long-term nutrient-depletion in the environment, but its adaptive mechanisms remain poorly understood. Here, …

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A 28-years evolution experiment on Burkholderia pseudomallei survival in nutrient-depleted sterile water

Chalita Chomkatekaew

Burkholderia pseudomallei is an environmental bacterium and the causative agent of neglected but often fatal infectious disease "Melioidosis". This bacterium can persist across diverse ecological niches, including prolonged exposure to nutrient-depleted environments. However, its adaptive strategies remain pooly understood. Here, we leveraged …

A 28-years evolution experiment on Burkholderia pseudomallei survival in nutrient-depleted sterile water

Chalita Chomkatekaew

Burkholderia pseudomallei is an environmental bacterium and the causative agent of neglected but often fatal infectious disease "Melioidosis". This bacterium can persist across diverse ecological niches, including prolonged exposure to nutrient-depleted environments. However, its adaptive strategies remain pooly understood. Here, we leveraged …

A 28-years evolution experiment on Burkholderia pseudomallei survival in nutrient-depleted sterile water

Chalita Chomkatekaew

Burkholderia pseudomallei is an environmental bacterium and the causative agent of neglected but often fatal infectious disease "Melioidosis". This bacterium can persist across diverse ecological niches, including prolonged exposure to nutrient-depleted environments. However, its adaptive strategies remain pooly understood. Here, we leveraged …

CRISPR-based environmental detection of Burkholderia pseudomallei identifies sanitation gaps and melioidosis risk in northeast Thailand

Sukritpong Pakdeerat, Chalita Chomkatekaew, Phumrapee Boonklang, Arin Wongprommoon et autres

Abstract Environmental exposure to Burkholderia pseudomallei , the causative agent of melioidosis, remains poorly characterised due to the limited sensitivity of conventional detection methods. This hinders accurate risk mapping and delays public health responses. Here, we developed CRISPR-BEEPs – a sensitive, equipment-light …

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Genetic diversity, determinants, and dissemination of Burkholderia pseudomallei lineages implicated in melioidosis in Northeast Thailand

Rathanin Seng, Chalita Chomkatekaew, Sarunporn Tandhavanant, Natnaree Saiprom et autres

Melioidosis is an often-fatal neglected tropical disease caused by an environmental bacterium Burkholderia pseudomallei. However, our understanding of the disease-causing bacterial lineages, their dissemination, and adaptive mechanisms remains limited. To address this, we conduct a comprehensive genomic analysis of 1,391 B. pseudomallei …

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Benchmarking CRISPR-BP34 for point-of-care melioidosis detection in low-income and middle-income countries: a molecular diagnostics study

Sukripong Pakdeerat, Phumrapee Boonklang, Kesorn Angchagun, Chalita Chomkatekaew et autres

BACKGROUND: Melioidosis is a neglected but often fatal tropical disease. The disease has broad clinical manifestations, which makes diagnosis challenging and time consuming. To improve diagnosis, we aimed to evaluate the performance of the CRISPR-Cas12a system (CRISPR-BP34) to detect Burkholderia pseudomallei DNA …

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Monitoring pathogens in wastewater

Arin Wongprommoon, Chalita Chomkatekaew, Claire Chewapreecha

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BurkHostGEN: a study protocol for evaluating variations in the Burkholderia pseudomallei and host genomes associated with melioidosis infection

Kesorn Angchagun, Phumrapee Boonklang, Chalita Chomkatekaew, Sukritpong Pakdeerat et autres

Background Melioidosis is a frequently fatal disease caused by an environmental bacterium Burkholderia pseudomallei . The disease is prevalent in northeast Thailand, particularly among rice field farmers who are at risk of bacterial exposure through contact with contaminated soil and water. However, …

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BurkHostGEN: a study protocol for evaluating variations in the Burkholderia pseudomallei and host genomes associated with melioidosis infection

Kesorn Angchagun, Phumrapee Boonklang, Chalita Chomkatekaew, Sukritpong Pakdeerat et autres

Background: Melioidosis is a frequently fatal disease caused by an environmental bacterium Burkholderia pseudomallei . The disease is prevalent in northeast Thailand, particularly among rice field farmers who are at risk of bacterial exposure through contact with contaminated soil and water. However, …