Iris Chiara Salaroglio

Interleukin-1 receptor-associated kinase 1 controls chemoresistance and immuno-killing in non-small cell lung cancer

Sabrina Digiovanni, Claudia Giannotta, Luca Alessandrì, Simona Fontana et autres

Non-small cell lung cancer (NSCLC) shows partial chemoimmunoresistance, driven by the upregulation of the drug efflux transporters ABCB1 and ABCC1 and the downregulation of the immunosensitizer protein ABCA1. To elucidate the molecular mechanisms underlying this chemoimmunoresistant phenotype, by performing a CRISPR-KO kinome …

it, fr, tr (code pays fourni par la source)

Targeting a novel metabolic vulnerability of non-small cell lung cancer to fatty acid oxidation surmounts chemoresistance

Simona Fontana, Dimas Carolina Belisario, Claudia Giannotta, Luca Alessandrì et autres

Aims Non-small cell lung cancer (NSCLC) patients treated with platinum drugs develop chemoresistance. C/EBPβ has alternative translational LAP and LIP isoforms which impact cancer chemoresistance by modulating ABC efflux transporter expression and activity. Differential alternative translation of LAP:LIP reprograms metabolism in murine …

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Endodontic Treatment of Apical Periodontitis Mitigates Systemic Inflammation and Restores the Blood–Brain Barrier Integrity in an In Vitro Model

Loredana Bergandi, Mario Alovisi, Elena Tachis, Nicola Scotti et autres

AIM: A growing body of research supports an association between periapical inflammation and an increased risk of various systemic effects. However, there is currently no scientific evidence demonstrating a causal relationship between this inflammation and changes in epithelial or endothelial permeability in …

it, us (code pays fourni par la source)

Modeling blood–brain barrier–glioblastoma interactions: implications for chemoresistance and therapeutic targeting

Mohamed Abd Naceur Ammar, Simona Dobiasová, Iris Chiara Salaroglio, Sihem Hmissa et autres

Glioblastoma (GBM) is the most aggressive and common primary brain tumor in adults and remains associated with a dismal prognosis despite standard-of-care treatment. A major contributor to therapeutic failure is the blood-brain barrier (BBB), which severely restricts the penetration of most therapeutic …

it, Tunisie (code pays fourni par la source)

Supplementary Table S3 from Autocrine 17-β-Estradiol/Estrogen Receptor-α Loop Determines the Response to Immune Checkpoint Inhibitors in Non–Small Cell Lung Cancer

Dario P. Anobile, Iris Chiara Salaroglio, Fabrizio Tabbò, Sofia La Vecchia et autres

Cell lines used

Supplementary Figure S7 from Autocrine 17-β-Estradiol/Estrogen Receptor-α Loop Determines the Response to Immune Checkpoint Inhibitors in Non–Small Cell Lung Cancer

Dario P. Anobile, Iris Chiara Salaroglio, Fabrizio Tabbò, Sofia La Vecchia et autres

Levels of FSHR and LHR in non-small cell lung cancer cells FSHR (panel a) and LHR (panel b) mRNA, measured by RT-PCR (technical triplicates), in female (F)-derived NCI-H1385 and NCI-H1975 cells, and in male (M)-derived A549 and NCI-H1650 cells. The relative expression …

Supplementary Figure S6 from Autocrine 17-β-Estradiol/Estrogen Receptor-α Loop Determines the Response to Immune Checkpoint Inhibitors in Non–Small Cell Lung Cancer

Dario P. Anobile, Iris Chiara Salaroglio, Fabrizio Tabbò, Sofia La Vecchia et autres

Levels of ERβ and ERα target genes in non-small cell lung cancer cells a. ERβ mRNA, measured by RT-PCR (technical triplicates), in female (F)-derived NCI-H1385 and NCI-H1975 cells, and in male (M)-derived A549 and NCI-H1650 cells. The relative expression of ERβ in …

Supplementary Figure S13 from Autocrine 17-β-Estradiol/Estrogen Receptor-α Loop Determines the Response to Immune Checkpoint Inhibitors in Non–Small Cell Lung Cancer

Dario P. Anobile, Iris Chiara Salaroglio, Fabrizio Tabbò, Sofia La Vecchia et autres

Switch-off/on EGFR activity in NCI-H1385 cells. a. Experimental scheme: Low EGFR activitywild-type NCI-H1385 cells were knocked-out (KO) for endogenous EGFR by a CRISPR/Cas vector, then stably transfected with an expression vector encoding L858R EGFR. b. Immunoblot of EGFR in wild-type (wt) NCI-H1385 …

Supplementary Figure S1 from Autocrine 17-β-Estradiol/Estrogen Receptor-α Loop Determines the Response to Immune Checkpoint Inhibitors in Non–Small Cell Lung Cancer

Dario P. Anobile, Iris Chiara Salaroglio, Fabrizio Tabbò, Sofia La Vecchia et autres

Outcome of non-small cell lung cancer patients, disaggregated according to gender, PD-L1 and ERα levels. Best response (PD: progressive disease; PR: partial response; SD: stable disease) after 6 months from the beginning of pembrolizumab treatment as first-line monotherapy, PFS and OS (Kapan-Meier …

Supplementary Figure S3 from Autocrine 17-β-Estradiol/Estrogen Receptor-α Loop Determines the Response to Immune Checkpoint Inhibitors in Non–Small Cell Lung Cancer

Dario P. Anobile, Iris Chiara Salaroglio, Fabrizio Tabbò, Sofia La Vecchia et autres

Outcome of non-small cell lung cancer patients in LUAD TCGA cohort. a. OS of patients of lung adenocarcinoma (LUAD) dataset of The Cancer Genome Atlas (TCGA), stratified according to the gender. F: females; M: males. b-c. OS of LUAD TCGA female and …

Supplementary Table S4 from Autocrine 17-β-Estradiol/Estrogen Receptor-α Loop Determines the Response to Immune Checkpoint Inhibitors in Non–Small Cell Lung Cancer

Dario P. Anobile, Iris Chiara Salaroglio, Fabrizio Tabbò, Sofia La Vecchia et autres

Primers list for RT-PCR

Supplementary Figure S15 from Autocrine 17-β-Estradiol/Estrogen Receptor-α Loop Determines the Response to Immune Checkpoint Inhibitors in Non–Small Cell Lung Cancer

Dario P. Anobile, Iris Chiara Salaroglio, Fabrizio Tabbò, Sofia La Vecchia et autres

Letrozole sensitizes xenografts to a second cycle of pembrolizumab by modulating the tumor-associated immune-environment. 60 Hu-CD34+NSG mice bearing female-derived ERα lowNCI-H11385 and ERα highNCI-H1975 tumors, male-derived ERα lowA549 and ERα highNCI-H1650 tumors (15/tumor type) were treated for 5 weeks with pembrolizumab+letrozole with …