Supplementary Figure S6 from Autocrine 17-β-Estradiol/Estrogen Receptor-α Loop Determines the Response to Immune Checkpoint Inhibitors in Non–Small Cell Lung Cancer

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Levels of ERβ and ERα target genes in non-small cell lung cancer cells a. ERβ mRNA, measured by RT-PCR (technical triplicates), in female (F)-derived NCI-H1385 and NCI-H1975 cells, and in male (M)-derived A549 and NCI-H1650 cells. The relative expression of ERβ in NCI-H1385 cells was considered 1. Data are means+SD (n=3). ***p<0.001: NCI-H1975 cells versus NCI-H1385; °°p<0.01: M-derived cells versus mean of F-derived cells (ANOVA). b. Expression of ERβ mRNA, measured by RT-PCR (technical triplicates), plotted versus expression of surface PD-L1, measured by flow cytometry (technical triplicates), in 29 human NSCLC cell lines derived from F or M patients. c. Expression of ERα-target genes CXCL12, IGFBP4 and ABCA3, measured by RT-PCR (technical triplicates). The relative expression of each gene in NCI-H1385 cells was considered 1. Data are means+SD (n=3). *p<0.05, ***p<0.001: NCI-H1975 cells versus NCI-H1385; °p<0.05, °°p<0.01, °°°p<0.001: M-derived cells versus mean of F-derived cells (ANOVA).

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