Yinan Wan

Imaging cellular activity across all organs reveals body-wide circuits

Virginia M. S. Ruetten, Wei Zheng, Igor Siwanowicz, Brett D. Mensh et autres

Abstract An animal’s ability to survive and thrive—whether fleeing from danger, eating a meal, or fighting an infection—arises from the collective moment-to-moment activity of many interacting cell types throughout the body. Physiology seeks to elucidate these cellular interactions that span organs, cell …

us, gb, cn, de, ch (code pays fourni par la source)

FOCUS-3D: Robust, generalizable volumetric cell segmentation for three-dimensional fluorescence microscopy

Qinghua Zhang, Zeyu Mu, Boqi Liu, Yunfeng Chi et autres

Abstract Understanding how cells establish spatial organization within tissues is a fundamental question in life sciences. While modern three-dimensional fluorescence microscopy captures large-volume tissue architecture, extracting quantitative cellular insights from complex volumetric datasets remains a major barrier. Here, we introduce FOCUS-3D, a …

cn, ch (code pays fourni par la source)

Odor preference maps to cohesive transcriptional domains in the olfactory bulb

Oded Mayseless, Joaquín Navajas Acedo, Yinan Wan, Vincent Hahaut et autres

Animals classify odors along behaviorally relevant dimensions, yet how the olfactory bulb, the first stage of olfactory processing, is organized and how this organization relates to odor classification remains unclear. Here, we identify cohesive transcriptional domains within the larval zebrafish olfactory bulb …

ch (code pays fourni par la source)

Hedgehog-driven adaxial cell constriction patterns slow muscle fate and somite boundary remodeling in the presomitic mesoderm

Ying Wang, X X Li, Rui Xia, Yinan Wan et autres

How morphogen signaling interfaces with cell behaviors and mechanics to coordinate body axis patterning has remained largely unclear. Here, we uncover a mechanochemical program that directs slow muscle fate commitment and somite boundary remodeling in the presomitic mesoderm (PSM). Hedgehog, secreted from …

cn, ch, sg (code pays fourni par la source)

High-Fidelity Long-term Whole-embryo Lineage and Fate Reconstruction by Iterative Tracking with Error Correction

Mengfan Wang, Qinghua Zhang, Congchao Wang, Yunfeng Chi et autres

Abstract Reconstructing the complete cell lineages and fate maps of a living embryo has remained a central challenge in developmental biology. Here we introduce ITEC (Iterative Tracking with Error Correction), a fully unsupervised method that automatically reconstructs the lineage of every cell …

us, cn, ch (code pays fourni par la source)

Whole-embryo spatial transcriptomics at subcellular resolution from gastrulation to organogenesis

Yinan Wan, Jakob El Kholtei, Ignatius Jenie, Mariona Colomer-Rosell et autres

Gene expression patterns underlie development, but their systematic detection in whole embryos has remained elusive. We introduce a whole-embryo imaging platform using multiplexed error-robust fluorescent in situ hybridization (weMERFISH). We quantified the expression of 495 genes in zebrafish embryos at subcellular resolution …

ch, us, cn, ca (code pays fourni par la source)

Imaging cellular activity simultaneously across all organs of a vertebrate reveals body-wide circuits

Virginia M. S. Ruetten, Weihao Zheng, Igor Siwanowicz, Brett D. Mensh et autres

Abstract All cells in an animal collectively ensure, moment-to-moment, the survival of the whole organism in the face of environmental stressors 1,2 . Physiology seeks to elucidate the intricate network of interactions that sustain life, which often span multiple organs, cell types, …

us, gb, cn, de, ch (code pays fourni par la source)

Whole-embryo Spatial Transcriptomics at Subcellular Resolution from Gastrulation to Organogenesis

Yinan Wan, Jakob El Kholtei, Ignatius Jenie, Mariona Colomer-Rosell et autres

Abstract Spatiotemporal patterns of gene expression underlie embryogenesis. Despite progress in single-cell genomics, mapping these patterns across whole embryos with comprehensive gene coverage and at high resolution has remained elusive. Here, we introduce a w hole- e mbryo imaging platform using m …

ch, us, ca, cn (code pays fourni par la source)

Analyzing the Impact of the Facebook-Cambridge Analytica Data Scandal on the US Tech Stock Market: A Cluster-Based Event Study

Vahidin Jeleskovic, Yinan Wan

This study delves into the intra-industry effects following a firm-specific scandal, with a particular focus on the Facebook data leakage scandal and its associated events within the U.S. tech industry and two additional relevant groups. We employ various metrics including daily spread, …

de (code pays fourni par la source)

The impact of Facebook-Cambridge Analytica data scandal on the USA tech stock market: An event study based on clustering method

Vahidin Jeleskovic, Yinan Wan

This study delves into the intra-industry effects following a firm-specific scandal, with a particular focus on the Facebook data leakage scandal and its associated events within the U.S. tech industry and two additional relevant groups. We employ various metrics including daily spread, …

Automated reconstruction of whole-embryo cell lineages by learning from sparse annotations

Caroline Malin-Mayor, Peter Bernhard Hirsch, Léo Guignard, Katie McDole et autres

We present a method to automatically identify and track nuclei in time-lapse microscopy recordings of entire developing embryos. The method combines deep learning and global optimization. On a mouse dataset, it reconstructs 75.8% of cell lineages spanning 1 h, as compared to …

us, de, fr, gb, ch (code pays fourni par la source)

Engineering the Fairness of Blackjack

Yinan Wan

Blackjack is a popular card game and is a widely played casino banking game. To win the blackjack, the players aim at getting a higher total value of the cards than the dealer, while not exceeding 21. The current rule of blackjack …