Léo Guignard

A quantitative pipeline for whole-mount deep imaging and analysis of multi-layered organoids across scales

Alice Gros, Jules Vanaret, Valentin Dunsing, Agathe Rostan et autres

Whole-mount 3D imaging at the cellular scale is a powerful tool for exploring complex processes during morphogenesis. In organoids, it allows examining tissue architecture, cell types, and morphology simultaneously in 3D models. However, cell packing in multilayered organoid tissues hinders both deep …

fr, us (code pays fourni par la source)

Author response: A quantitative pipeline for whole-mount deep imaging and analysis of multi-layered organoids across scales

Alice Gros, Jules Vanaret, Valentin Dunsing, Agathe Rostan et autres

Whole-mount 3D imaging at the cellular scale is a powerful tool for exploring complex processes during morphogenesis. In organoids, it allows examining tissue architecture, cell types, and morphology simultaneously in 3D models. However, cell packing in multilayered organoid tissues hinders both deep …

Reviewer #2 (Public review): A quantitative pipeline for whole-mount deep imaging and analysis of multi-layered organoids across scales

Alice Gros, Jules Vanaret, Valentin Dunsing, Agathe Rostan et autres

Whole-mount 3D imaging at the cellular scale is a powerful tool for exploring complex processes during morphogenesis. In organoids, it allows examining tissue architecture, cell types, and morphology simultaneously in 3D models. However, cell packing in multilayered organoid tissues hinders both deep …

Reviewer #3 (Public review): A quantitative pipeline for whole-mount deep imaging and analysis of multi-layered organoids across scales

Alice Gros, Jules Vanaret, Valentin Dunsing, Agathe Rostan et autres

Whole-mount 3D imaging at the cellular scale is a powerful tool for exploring complex processes during morphogenesis. In organoids, it allows examining tissue architecture, cell types, and morphology simultaneously in 3D models. However, cell packing in multilayered organoid tissues hinders both deep …

fr, us (code pays fourni par la source)

A quantitative pipeline for whole-mount deep imaging and analysis of multi-layered organoids across scales

Alice Gros, Jules Vanaret, Valentin Dunsing, Agathe Rostan et autres

Whole-mount 3D imaging at the cellular scale is a powerful tool for exploring complex processes during morphogenesis. In organoids, it allows examining tissue architecture, cell types, and morphology simultaneously in 3D models. However, cell packing in multilayered organoid tissues hinders both deep …

fr, us (code pays fourni par la source)

Reviewer #1 (Public review): A quantitative pipeline for whole-mount deep imaging and analysis of multi-layered organoids across scales

Alice Gros, Jules Vanaret, Valentin Dunsing, Agathe Rostan et autres

Whole-mount 3D imaging at the cellular scale is a powerful tool for exploring complex processes during morphogenesis. In organoids, it allows examining tissue architecture, cell types, and morphology simultaneously in 3D models. However, cell packing in multilayered organoid tissues hinders both deep …

sc3D: A Comprehensive Tool for 3D Spatial Transcriptomic Analysis

Miquel Peiro Sendra, Adriano Bolondi, Léo Guignard

gene expression. These results show that sc3D allows reproducible reconstruction and analysis of volumetric spatial omics data across different samples and experimental platforms. Key features • 3D reconstruction: sc3D aligns serial Slide-seq arrays and interpolates between sections to generate volumetric transcriptomic datasets …

fr, es (code pays fourni par la source)

Tapenade: Spatial Quantification of Mechanical and Genetic Fields in Dense 3D Organoids From Cell to Tissue Scale

Alice Gros, Jules Vanaret, Sham Tlili, Léo Guignard

Author response: Longitudinal tracking of neuronal activity from the same cells in the developing brain using Track2p

Jure Majnik, Manon Mantez, Sofia Zangila, Stéphane Bugeon et autres

A novel algorithm allowed for the continuous tracking of neurons throughout early postnatal development, revealing a transition in neural firing statistics coinciding with the onset of behaviour-dependent activity.

fr (code pays fourni par la source)

Fast volumetric fluorescence lifetime imaging of multicellular systems using single-objective light-sheet microscopy

Valentin Dunsing, Johan Hummert, Claire Chardès, Thomas Schönau et autres

Fluorescence lifetime imaging (FLIM) is widely used for functional and multiplexed bioimaging. The lifetime of autofluorescence or fluorescent sensors encodes physiologically relevant parameters. Thus, FLIM is especially relevant for the investigation of living systems. However, application of FLIM to live specimen is …

fr, us, de, gb, sg, ch (code pays fourni par la source)

Longitudinal tracking of neuronal activity from the same cells in the developing brain using Track2p

Jure Majnik, Manon Mantez, Sofia Zangila, Stéphane Bugeon et autres

Understanding cortical circuit development requires tracking neuronal activity across days in the growing brain. While in vivo calcium imaging now enables such longitudinal studies, automated tools for reliably tracking large populations of neurons across sessions remain limited. Here, we present a novel …

fr, us (code pays fourni par la source)

Author response: Longitudinal tracking of neuronal activity from the same cells in the developing brain using Track2p

Jure Majnik, Manon Mantez, Sofia Zangila, Stéphane Bugeon et autres