Jakob El Kholtei

Whole-embryo spatial transcriptomics at subcellular resolution from gastrulation to organogenesis

Yinan Wan, Jakob El Kholtei, Ignatius Jenie, Mariona Colomer-Rosell et autres

Gene expression patterns underlie development, but their systematic detection in whole embryos has remained elusive. We introduce a whole-embryo imaging platform using multiplexed error-robust fluorescent in situ hybridization (weMERFISH). We quantified the expression of 495 genes in zebrafish embryos at subcellular resolution …

ch, us, cn, ca (code pays fourni par la source)

Nodal Signaling: A Paradigm for TGFβ Signaling in Embryonic Development

Jakob El Kholtei, Mireia Codina-Tobias, Alexander Franz Schier

Nodal signaling molecules are TGFβ family ligands that arose early during bilaterian evolution and are crucial for several key steps in embryonic development. They regulate the specification of mesoderm and endoderm, body patterning, left-right asymmetry, and pluripotency maintenance. Many of Nodal's effects …

ch (code pays fourni par la source)

Whole-embryo Spatial Transcriptomics at Subcellular Resolution from Gastrulation to Organogenesis

Yinan Wan, Jakob El Kholtei, Ignatius Jenie, Mariona Colomer-Rosell et autres

Abstract Spatiotemporal patterns of gene expression underlie embryogenesis. Despite progress in single-cell genomics, mapping these patterns across whole embryos with comprehensive gene coverage and at high resolution has remained elusive. Here, we introduce a w hole- e mbryo imaging platform using m …

ch, us, ca, cn (code pays fourni par la source)

Space-time logic of liver gene expression at sub-lobular scale

Colas Droin, Jakob El Kholtei, Keren Bahar Halpern, Clémence Hurni et autres

ch, il (code pays fourni par la source)

Emergence of Neuronal Diversity during Vertebrate Brain Development

Bushra Raj, Jeffrey A. Farrell, Jialin Liu, Jakob El Kholtei et autres

us, ch (code pays fourni par la source)

Space-time logic of liver gene expression at sublobular scale

Colas Droin, Jakob El Kholtei, Keren Bahar Halpern, Clémence Hurni et autres

Abstract The mammalian liver performs key physiological functions for maintaining energy and metabolic homeostasis. Liver tissue is both spatially structured and temporally orchestrated. Hepatocytes operate in repeating anatomical units termed lobules and different lobule zones perform distinct functions. The liver is also …

ch, il (code pays fourni par la source)

Reconstructing complex lineage trees from scRNA-seq data using MERLoT

R. Gonzalo Parra, Nikolaos Papadopoulos, Laura Ahumada-Arranz, Jakob El Kholtei et autres

Advances in single-cell transcriptomics techniques are revolutionizing studies of cellular differentiation and heterogeneity. It has become possible to track the trajectory of thousands of genes across the cellular lineage trees that represent the temporal emergence of cell types during dynamic processes. However, …

de, ch (code pays fourni par la source)

Reconstructing complex lineage trees from scRNA-seq data using MERLoT

R. Gonzalo Parra, Nikolaos Papadopoulos, Laura Ahumada-Arranz, Jakob El Kholtei et autres

Abstract Advances in single-cell transcriptomics techniques are revolutionizing studies of cellular differentiation and heterogeneity. Consequently, it becomes possible to track the trajectory of thousands of genes across the cellular lineage trees that represent the temporal emergence of cell types during dynamic processes. …

de (code pays fourni par la source)