Evita Balducci

Distinct effects of adjuvants on B cell responses to protein or polysaccharide antigens contained in glycoconjugate vaccines

Sonia Budroni, Elisa Faenzi, Simona Tavarini, Chiara Sammicheli et autres

Background Protein-polysaccharide conjugate vaccines rely on the induction of T-cell-dependent responses that support germinal center (GC) reactions to potentiate the expansion of antigen-specific memory B-cell (MBC) populations and high-avidity antibody responses. The effects of adjuvants on B-cell and antibody responses are well …

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Polysaccharides’ Structures and Functions in Biofilm Architecture of Antimicrobial-Resistant (AMR) Pathogens

Evita Balducci, Francesco Papi, Daniela Eloisa Capialbi, Linda Del Bino

Bacteria and fungi have developed resistance to the existing therapies such as antibiotics and antifungal drugs, and multiple mechanisms are mediating this resistance. Among these, the formation of an extracellular matrix embedding different bacterial cells, called biofilm, is an effective strategy through …

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Chemical Synthesis and Immunological Evaluation of Fragments of the Multiantennary Group-Specific Polysaccharide of Group B Streptococcus

Zhen Wang, Jacopo Enotarpi, Giada Buffi, Alfredo Pezzicoli et autres

(GBS) is a Gram-positive bacterium and the most common cause of neonatal blood and brain infections. At least 10 different serotypes exist, that are characterized by their different capsular polysaccharides. The Group B carbohydrate (GBC) is shared by all serotypes and therefore …

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Retaining the structural integrity of disulfide bonds in diphtheria toxoid carrier protein is crucial for the effectiveness of glycoconjugate vaccine candidates

Filippo Carboni, Annabel Kitowski, Charlotte Sorieul, Daniele Veggi et autres

The introduction of glycoconjugate vaccines marks an important point in the fight against various infectious diseases. The covalent conjugation of relevant polysaccharide antigens to immunogenic carrier proteins enables the induction of a long-lasting and robust IgG antibody response, which is not observed …

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Elucidating the Structural and Minimal Protective Epitope of the Serogroup X Meningococcal Capsular Polysaccharide

Gian Pietro Pietri, Marta Tontini, Barbara Brogioni, Davide Oldrini et autres

Despite the considerable progress toward the eradication of meningococcal disease with the introduction of glycoconjugate vaccines, previously unremarkable serogroup X has emerged in recent years, recording several outbreaks throughout the African continent. Different serogroup X polysaccharide-based vaccines have been tested in preclinical …

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Group B Streptococcus chimeric capsular polysaccharides as novel multivalent vaccine candidates

Edmondo Campisi, Roberto Rosini, Maria Rosaria Romano, Evita Balducci et autres

The capsular polysaccharide of the human pathogen Group B Streptococcus is a key virulence factor and vaccine candidate that induces protective antibodies when conjugated to carrier proteins. It consists of long polymeric chains of oligosaccharide repeating units, and each of the ten …

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Publisher Correction: A stabilized glycomimetic conjugate vaccine inducing protective antibodies against Neisseria meningitidis serogroup A

Jacopo Enotarpi, Marta Tontini, Cristiana Balocchi, Daan van der Es et autres

A Correction to this paper has been published: https://doi.org/10.1038/s41467-020-20120-4.

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A stabilized glycomimetic conjugate vaccine inducing protective antibodies against Neisseria meningitidis serogroup A

Jacopo Enotarpi, Marta Tontini, Cristiana Balocchi, Daan van der Es et autres

Abstract Neisseria meningitidis serogroup A capsular polysaccharide (MenA CPS) consists of (1 → 6)-2-acetamido-2-deoxy-α-D-mannopyranosyl phosphate repeating units, O -acetylated at position C3 or C4. Glycomimetics appear attractive to overcome the CPS intrinsic lability in physiological media, due to cleavage of the phosphodiester …

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Novel Multiplex Immunoassays for Quantification of IgG against Group B Streptococcus Capsular Polysaccharides in Human Sera

Giada Buffi, Bruno Galletti, Maria Laura Stella, Daniela Proietti et autres

Group B streptococcal infections can cause death in neonates up to 3 months of age. Intrapartum antibiotic prophylaxis in GBS-colonized mothers has limited early infections but has no impact after the first week of life. The development of a maternal vaccine to …

Characterization of the Conjugation Pattern in Large Polysaccharide–Protein Conjugates by NMR Spectroscopy

Stefano Giuntini, Evita Balducci, Linda Cerofolini, Enrico Ravera et autres

Carbohydrate-based vaccines are among the safest and most effective vaccines and represent potent tools for prevention of life-threatening bacterial infectious diseases, like meningitis and pneumonia. The chemical conjugation of a weak antigen to protein as a source of T-cell epitopes generates a …

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Characterization of the Conjugation Pattern in Large Polysaccharide–Protein Conjugates by NMR Spectroscopy

Stefano Giuntini, Evita Balducci, Linda Cerofolini, Enrico Ravera et autres

Abstract Carbohydrate‐based vaccines are among the safest and most effective vaccines and represent potent tools for prevention of life‐threatening bacterial infectious diseases, like meningitis and pneumonia. The chemical conjugation of a weak antigen to protein as a source of T‐cell epitopes generates …

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Streptococcus agalactiae Capsule Polymer Length and Attachment Is Determined by the Proteins CpsABCD

Chiara Toniolo, Evita Balducci, Maria Rosaria Romano, Daniela Proietti et autres

Background: The polysaccharide capsule is a major virulence factor of Streptococcus agalactiae . Results: Mutations of the genes cpsABCD result in aberrant capsule length and localization. Conclusion: The CpsABCD proteins form a system that modulates termination of capsule elongation. Significance: This work …

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