Federica Forbici

Impact of SARS-CoV-2 Pandemic on Neuroinfectious Etiology in the Lazio Region: Evidence from Laboratory-Based Surveillance Analysis

Martina Rueca, Sara Leone, Maria Beatrice Valli, Gabriella De Carli et autres

The mitigation measures adopted to reduce the spread of COVID-19 have not only impacted the transmission of respiratory infections but also other infectious diseases. In this study, we explored the effect of the pandemic on the epidemiology of Central Nervous System (CNS) …

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Ion Torrent Genexus as a Fast and Reliable Solution for HIV-1 Drug Resistance Testing: Comparison with the GeneStudio S5 Workflow

Flavia Smoquina, Federica Forbici, Giulia Berno, Martina Rueca et autres

Next-generation sequencing (NGS) has improved HIV-1 genotypic resistance testing (GRT) by enabling the detection of minority drug-resistance variants, although interpretation of low-frequency mutations remains challenging because of sequencing artifacts. This study compared the analytical performance and workflow efficiency of two Ion Torrent …

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Expression of Human Endogenous Retroviruses in Peripheral Blood of Acute and Chronically HIV-Infected Subjects and Effect of Antiretroviral Therapy

Elisabetta Lazzari, Gabriella Rozera, Lucrezia Pierfederici, Daniele Pietrucci et autres

Human endogenous retroviruses (HERVs) originate from ancient retroviral integration into the primate germline. Although most are defective proviruses, the most recently endogenized groups, like the HERV-K family, retain intact ORFs encoding retroviral proteins. HERVs usually remain transcriptionally silent, yet this status is …

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Effectiveness of Switching to Bictegravir/Emtricitabine/Tenofovir Alafenamide from Rilpivirine or Other Non-nucleoside Reverse Transcriptase Inhibitor (NNRTI)-Based Antiretroviral Therapy in Virologically Suppressed People with HIV: A Retrospective Analysis (DRIVE-SWITCH Study)

Roberta Gagliardini, Alessandro Caioli, Marisa Fusto, Sandrine Ottou et autres

INTRODUCTION: Extensive real-world data in people with HIV (PWH) switching from non-nucleoside reverse transcriptase inhibitors (NNRTIs), in particular from rilpivirine (RPV)-based regimens to bictegravir/emtricitabine/tenofovir alafenamide (B/F/TAF), are lacking. METHODS: This is a single-center retrospective study. Inclusion criteria were PWH aged ≥ 18 …

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Atypical HIV-1 Viremia Persistently Detected Exclusively Through the Pol Target by a Dual-Target (Pol and LTR) Assay: A Case Report About a New Diagnostic Challenge

Alessandra Amendola, Sara Belladonna, Flavia Smoquina, Giulia Capecchi et autres

We report the case of a 48-year-old man admitted with severe pneumonia, profound immunosuppression and multiple co-infections, showing unusual pattern of HIV-1 viremia. With the Aptima HIV-1 Quant Dx assay, a dual-target diagnostic assay for monitoring of viral RNA in people living …

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Improved HIV-1 Subtyping Accuracy Using near Full-Length Sequencing: A Comparison of Common Tools

Flavia Smoquina, Giulia Berno, Federica Forbici, Giuseppe Sberna et autres

The extensive genetic diversity of HIV-1, also represented by the circulation of multiple subtypes and circulating recombinant forms (CRFs), poses significant challenges for accurate subtype classification, especially when sequencing is limited to partial genomic regions. This study evaluated the performance of four …

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P60 Near full-length sequencing is able to ensure a more accurate HIV-1 subtyping: comparison with commonly used methods

Flavia Smoquina, Giulia Berno, Federica Forbici, Gian Sberna et autres

SC16 Analysis of HIV proviral integration sites in the host genome of PWH with a virus displaying amino acid polymorphism in the integrase C-terminal region

Gian Sberna, Giulia Berno, Christin Gruber, Gabriella Rozera et autres

Investigation of Natural Resistance to Fostemsavir and Lenacapavir in Naïve Primary Infections by Ultra-Deep Sequencing of near Full-Length HIV-1 Genomes

Elisabetta Lazzari, Gabriella Rozera, Roberta Gagliardini, Valentina Mazzotta et autres

Next-generation sequencing (NGS) of near full-length HIV genomes was performed to investigate natural resistance to Fostemsavir (FTR) and Lenacapavir (LEN) at the quasispecies level in nine naïve primary HIV infections harboring different HIV subtypes and recombinant forms. Reconstructed genomes provided a median …

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HIV-1 transmitted drug resistance in newly diagnosed individuals in Italy over the period 2015–21

Lavinia Fabeni, Daniele Armenia, Isabella Abbate, Roberta Gagliardini et autres

BACKGROUND: Transmitted drug resistance (TDR) is still a critical aspect for the management of individuals living with HIV-1. Thus, its evaluation is crucial to optimize HIV care. METHODS: Overall, 2386 HIV-1 protease/reverse transcriptase and 1831 integrase sequences from drug-naïve individuals diagnosed in …

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Molecular Genotyping of Circulating Enterovirus in the Lazio Region from 2012 to 2023

Martina Rueca, Francesco Vairo, Martina Spaziante, Lavinia Fabeni et autres

Enteroviruses (EVs) are ubiquitous viruses that circulate worldwide, causing sporadic or epidemic infections, typically during the summer and fall. They cause a broad spectrum of illnesses, ranging from an unspecified febrile clinical presentation to a severe illness. EVs are recognized to be …

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P-215 Optimization of HIV-1 next generation sequencing genotyping drug resistance testing methods from both RNA and DNA on Ion GeneStudio S5 prime system

Lavinia Fabeni, Flavia Smoquina, Federica Forbici, Giulia Berno et autres

Background Genotyping drug resistance testing methods for HIV-1 are continually evolving, as exemplified by Next Generation Sequencing (NGS) technology, which is gradually replacing Sanger sequencing (SS) in clinical diagnostics. A comparison between SS and NGS was performed, considering both DNA and RNA …

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