Gabriella Rozera

Improved HIV-1 Subtyping Accuracy Using near Full-Length Sequencing: A Comparison of Common Tools

F Smoquina, Giulia Berno, Federica Forbici, Giuseppe Sberna et autres

The extensive genetic diversity of HIV-1, also represented by the circulation of multiple subtypes and circulating recombinant forms (CRFs), poses significant challenges for accurate subtype classification, especially when sequencing is limited to partial genomic regions. This study evaluated the performance of four …

it (code pays fourni par la source)

Expression of HCMV-Encoded miRNA in Subjects Acutely Coinfected with HIV: Correlation with Inflammation and Immune Activation

Elisabetta Lazzari, Gabriella Rozera, Rozenn Esvan, Roberta Gagliardini et autres

Human cytomegalovirus (HCMV) coinfection is associated with a faster HIV disease progression and adverse clinical outcomes. HCMV-encoded miRNA expression, in individuals acutely infected with HIV (AHI), compared to those with HCMV monoinfection, was investigated in relation to viral replication and inflammation/immune activation. …

it (code pays fourni par la source)

OC42 Key predictors of optimal viro-immunological response in primary HIV infection

Annalisa Mondi, Assunta Navarra, Roberta Gagliardini, Valentina Mazzotta et autres

P60 Near full-length sequencing is able to ensure a more accurate HIV-1 subtyping: comparison with commonly used methods

F Smoquina, Giulia Berno, Federica Forbici, Gian Sberna et autres

SC16 Analysis of HIV proviral integration sites in the host genome of PWH with a virus displaying amino acid polymorphism in the integrase C-terminal region

Gian Sberna, Giulia Berno, C. Gruber, Gabriella Rozera et autres

P92 Implementation of a same-day ART start model in a real-life setting: experiences from a clinical centre in Italy

Giovanni De Micheli, Alessandro Caioli, Rozenn Esvan, Alessandro Giacinta et autres

OC51 Expression of HCMV-encoded miRNAs in co-infected PLWH and relationships with inflammation/immune activation

Elisa de Lazzari, Gabriella Rozera, Rozenn Esvan, Roberta Gagliardini et autres

Investigation of Natural Resistance to Fostemsavir and Lenacapavir in Naïve Primary Infections by Ultra-Deep Sequencing of near Full-Length HIV-1 Genomes

Elisabetta Lazzari, Gabriella Rozera, Roberta Gagliardini, Valentina Mazzotta et autres

Next-generation sequencing (NGS) of near full-length HIV genomes was performed to investigate natural resistance to Fostemsavir (FTR) and Lenacapavir (LEN) at the quasispecies level in nine naïve primary HIV infections harboring different HIV subtypes and recombinant forms. Reconstructed genomes provided a median …

it, dk (code pays fourni par la source)

Cerebrospinal Fluid and Peripheral Blood Lymphomonocyte Single-Cell Transcriptomics in a Subject with Multiple Sclerosis Acutely Infected with HIV

Carmela Pinnetti, Gabriella Rozera, Francesco Messina, Pietro Giorgio Spezia et autres

Signatures of neurodegeneration in clinical samples from a subject with multiple sclerosis (MS) acutely infected with HIV were investigated with single-cell transcriptomics using 10X Chromium technology. Sequencing was carried out on NovaSeq-TM, and the analysis was performed with Cell Ranger software (v …

it (code pays fourni par la source)

Role of Direct Sexual Contact in Human Transmission of Monkeypox Virus, Italy

Giuseppe Sberna, Gabriella Rozera, Claudia Minosse, Licia Bordi et autres

The 2022 global mpox outbreak was driven by human-to-human transmission, but modes of transmission by sexual relationship versus sexual contact remain unclear. We evaluated sexual transmission of mpox by using monkeypox virus (MPXV) G2R-mRNA as a marker of ongoing viral replication through …

Rapid Determination of SARS-CoV-2 Integrity and Infectivity by Using Propidium Monoazide Coupled with Digital Droplet PCR

Giuseppe Sberna, Cosmina Mija, Eleonora Lalle, Gabriella Rozera et autres

SARS-CoV-2 is a highly infectious virus responsible for the COVID-19 pandemic. Therefore, it is important to assess the risk of SARS-CoV-2 infection, especially in persistently positive patients. Rapid discrimination between infectious and non-infectious viruses aids in determining whether prevention, control, and treatment …

it (code pays fourni par la source)

P-215 Optimization of HIV-1 next generation sequencing genotyping drug resistance testing methods from both RNA and DNA on Ion GeneStudio S5 prime system

Lavinia Fabeni, F Smoquina, Federica Forbici, Giulia Berno et autres

Background Genotyping drug resistance testing methods for HIV-1 are continually evolving, as exemplified by Next Generation Sequencing (NGS) technology, which is gradually replacing Sanger sequencing (SS) in clinical diagnostics. A comparison between SS and NGS was performed, considering both DNA and RNA …

it (code pays fourni par la source)