Isabel D. Alves

Real-time analysis of pore formation by bi-component staphylococcal leukotoxins using the two-electrode voltage-clamp technique

Laura Lemel, Simon HARRIS, Guillaume Audic, Laure Bellard et autres

Abstract Pore forming toxins (PFTs) are cytotoxins secreted in water-soluble form by pathogenic bacteria. They have the ability to form pores in the membrane of host cells, ultimately leading to cell death by lytic activity. Staphylococcus aureus produces a variety of bi-component …

fr, ca (code pays fourni par la source)

The impact of charge on chlorpromazine interaction with lipid membranes

Ana Gorše, Nicoló Paracini, Marion Mathelié-Guinlet, Victoria Lublin et autres

Psychiatric disorders, such as schizophrenia, are treated with antipsychotics. One such molecule is chlorpromazine (CPZ) that mainly exerts its therapeutic effect via its antagonist activity on the dopamine D2 receptor. Due to its high lipophilicity, it can partition and accumulate in membranes, …

fr (code pays fourni par la source)

The chemokine receptor-like fourth extracellular loop of the apelin receptor differentially regulates apelin and elabela binding and signalling

Chloé Bonef, Hela Bouhsine, Carine Huynh, Marion Chaigneau et autres

Background and Purpose Apelin and Elabela are endogenous ligands of the apelin receptor (ApelinR/APJ), a GPCR involved in cardiovascular regulation and body fluid homeostasis. Human contains a conserved disulfide bridge linking the N-terminal domain to extracellular loop 3 (ECL3) via Cys19 and …

fr, us (code pays fourni par la source)

BRET-based G Protein Biosensors for Measuring G Protein-Coupled Receptor Activity in Live Cells

Adeline Cœugnet, Hannes Schihada, Estelle Rascol, Isabel D. Alves et autres

G protein-coupled receptors (GPCRs) constitute the largest family of transmembrane receptors, playing a crucial role in cellular signaling by transducing extracellular stimuli into intracellular responses. GPCR activation leads to conformational changes that enable interactions with heterotrimeric G proteins. Upon activation, the Gα …

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Membrane Partition and Structural Reorganization Induced by Antipsychotics with Distinct Clinical Profiles

Ana Gorše, Vesela Yordanova, Jessica Bodosa, Marion Mathelié‐Guinlet et autres

Antipsychotics (APs) are used in the treatment of severe mental disorders. Their mechanism of action involves interaction with multiple brain targets, notably the dopamine D2 receptor (D2R), where they compete with dopamine. Due to their lipophilic nature, APs also partition and accumulate …

fr, bg, us, de, pt (code pays fourni par la source)

Repression of apelin Furin cleavage sites provides antimetastatic strategy in colorectal cancer

Béatrice Demoures, Fabienne Soulet, Jean Descarpentrie, Isabel Galeano-Otero et autres

The adipokine apelin has been directly implicated in various physiological processes during embryogenesis and human cancers. Nevertheless, the importance of the conversion of its precursor proapelin to mature apelin in tumorigenesis remains unknown. In this study, we identify Furin as the cellular …

fr, es (code pays fourni par la source)

Membrane partition and structural reorganization induced by anti-psychotics with distinct clinical profiles

Ana Gorše, Vesela Yordanova, Jessica Bodosa, Marion Mathelié‐Guinlet et autres

ABSTRACT Antipsychotics (APs) are used in the treatment of severe mental disorders. Their mechanism of action involves interaction with multiple brain targets, notably the dopamine D2 receptors (D2R), where they compete with dopamine. Due to their lipophilic nature, APs also partition and …

fr, bg, us, pt (code pays fourni par la source)

Membrane Partition and Structural Reorganization Induced by Antipsychotics with Distinct Clinical Profiles

Ana Gorše, Vesela Yordanova, Jessica Bodosa, Marion Mathelié‐Guinlet et autres

fr, bg, us, pt (code pays fourni par la source)

Author Reply to Peer Reviews of Membrane lipid poly-unsaturation selectively affects ligand induced dopamine D2 receptor internalization

Silvia Sposini, Rim Baccouch, Véronique De Smedt‐Peyrusse, Joyce Heuninck et autres

fr (code pays fourni par la source)

Interaction of full-length Tau with negatively charged lipid membranes leads to polymorphic aggregates

Vicky Ury‐Thiery, Yann Fichou, Isabel D. Alves, Michaël Molinari et autres

in the micromolar range, indicating the deposition of a thick protein layer. Atomic force microscopy (AFM) real-time imaging allows the observation of partial lipid solubilization and the deposition of polymorphic aggregates in the form of thick patches and fibrillary structures resembling amyloid …

fr (code pays fourni par la source)

Membrane lipid poly-unsaturation selectively affects dopamine D2 receptor internalization

Silvia Sposini, Rim Baccouch, Véronique De Smedt‐Peyrusse, Joyce Heuninck et autres

Abstract The brain is highly enriched in poly-unsaturated fatty acids (PUFAs) and their deficiency has been associated with several neuropsychiatric disorders. Here, we demonstrate that the Dopamine receptor D2 (D2R), a class A G protein coupled receptor (GPCR) which is a main …

fr, gb (code pays fourni par la source)

Chocolates Produced with Unroasted and Roasted Cocoa Beans: A Comparative Study of the Preservation of Bioactive Compounds

Rebeca Onelli, Josane Cardim de Jesus, Lucas Reis, Isabel D. Alves et autres

The aim of the present study was to evaluate the effect of roasting and cocoa mass content on the composition of chocolates. Chocolate formulations were developed (40, 50, 60 and 70% cocoa solids) using unroasted and roasted cocoa beans, and analyses were …

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