Laura Lemel

Real-time analysis of pore formation by bi-component staphylococcal leukotoxins using the two-electrode voltage-clamp technique

Laura Lemel, Simon HARRIS, Guillaume Audic, Laure Bellard et autres

Abstract Pore forming toxins (PFTs) are cytotoxins secreted in water-soluble form by pathogenic bacteria. They have the ability to form pores in the membrane of host cells, ultimately leading to cell death by lytic activity. Staphylococcus aureus produces a variety of bi-component …

fr, ca (code pays fourni par la source)

GRK5 regulates endocytosis of FPR2 independent of β-arrestins

Christine E. Jack, E. R. Cope, Laura Lemel, Meritxell Canals et autres

The formyl-peptide receptor 2 (FPR2) is a G-protein-coupled receptor that responds to pathogen-derived peptides and regulates both proinflammatory and proresolution cellular processes. While ligand selectivity and G-protein signaling of FPR2 have been well characterized, molecular mechanisms controlling subsequent events such as endocytosis …

gb, de, jp (code pays fourni par la source)

Assessment of the potential of novel and classical opioids to induce respiratory depression in mice

R. W. Hill, Julie Sanchez, Laura Lemel, Mirjana Antonijevic et autres

BACKGROUND AND PURPOSE: Opioid-induced respiratory depression limits the use of μ-opioid receptor agonists in clinical settings and is the main cause of opioid overdose fatalities. The relative potential of different opioid agonists to induce respiratory depression at doses exceeding those producing analgesia …

gb, us (code pays fourni par la source)

Interactions of cholesterol molecules with GPCRs in different states: A comparative analysis of GPCRs' structures

Christophe Moreau, Guillaume Audic, Laura Lemel, María Dolores García-Fernández et autres

fr (code pays fourni par la source)

OZITX, a pertussis toxin-like protein for occluding inhibitory G protein signalling including Gαz

Alastair C. Keen, Laura Lemel, Daniel J. Scott, Meritxell Canals et autres

Abstract Heterotrimeric G proteins are the main signalling effectors for G protein-coupled receptors. Understanding the distinct functions of different G proteins is key to understanding how their signalling modulates physiological responses. Pertussis toxin, a bacterial AB5 toxin, inhibits Gαi/o G proteins and …

gb, au, dk, us, jp (code pays fourni par la source)

The ligand-bound state of a G protein-coupled receptor stabilizes the interaction of functional cholesterol molecules

Laura Lemel, Katarzyna Nieścierowicz, María Dolores García-Fernández, Leonardo Darré et autres

Cholesterol is a major component of mammalian plasma membranes that not only affects the physical properties of the lipid bilayer but also is the function of many membrane proteins including G protein-coupled receptors. The oxytocin receptor (OXTR) is involved in parturition and …

fr, uy, it, gb, us (code pays fourni par la source)

GRKs as Key Modulators of Opioid Receptor Function

Laura Lemel, J. Robert Lane, Meritxell Canals

Understanding the link between agonist-induced phosphorylation of the mu-opioid receptor (MOR) and the associated physiological effects is critical for the development of novel analgesic drugs and is particularly important for understanding the mechanisms responsible for opioid-induced tolerance and addiction. The family of …

gb (code pays fourni par la source)

Développement de la technologie des récepteurs couplés à un canal ionique pour la caractérisation fonctionnelle des récepteurs couplés aux protéines G

Laura Lemel

Les recepteurs couples aux proteines G (RCPG) sont des proteines membranaires impliquees dans la communication entre cellules via des messagers circulants (hormones, neurotransmetteurs) ainsi que dans la perception de notre environnement (vision, odorat, gout). Ils sont essentiels a de nombreuses fonctions physiologiques …

Développement de la technologie des récepteurs couplés à un canal ionique pour la caractérisation fonctionnelle des récepteurs couplés aux protéines G

Laura Lemel

Les récepteurs couplés aux protéines G (RCPG) sont des protéines membranaires impliquées dans la communication entre cellules via des messagers circulants (hormones, neurotransmetteurs) ainsi que dans la perception de notre environnement (vision, odorat, goût). Ils sont essentiels à de nombreuses fonctions physiologiques …

fr (code pays fourni par la source)

Functional mapping of the N-terminal arginine cluster and C-terminal acidic residues of Kir6.2 channel fused to a G protein-coupled receptor

Maria Antonietta Principalli, Laura Lemel, Anaëlle Rongier, Anne-Claire Godet et autres

fr, gb (code pays fourni par la source)