Michael Lienhard Schmitz

Small molecule-mediated activation of Notch signal transduction

Subhamita Dey, Benedetto Daniele Giaimo, Iva Katharina Zöllner, Qingwen Yang et autres

Abstract The Notch signaling pathway is pivotal in regulating cell differentiation, stem cell maintenance and oncogenesis. While several Notch inhibitors have been developed, effective small molecule activators are scarce. Here, we identify a small molecule that robustly activates Notch signaling, leading to …

de (code pays fourni par la source)

A novel approach towards a histone replacement system in Tetrapods

Maximilian Pfisterer, Amelie Pritz, Anna Parry, Michael Lienhard Schmitz

Histone replacement systems are valuable tools for studying histone modifications, but in vertebrates this is so far only possible by labor-intensive CRISPR base editing of each single histone gene. To facilitate such studies, we developed an alternative method and conducted proof-of-principle experiments …

de (code pays fourni par la source)

High Precision Quantification of small RNA Slicing Activity - Native Index Ligation-based Targeted Degradome Sequencing (NIL-TDS)

B. Werner, Sabrine Nasfi, Michael Lienhard Schmitz, Manar Makhoul et autres

ABSTRACT RNA interference (RNAi) is an effective and precise regulatory mechanism in eukaryotes in which small RNAs mediate endonucleolytic slicing of complementary target mRNAs. Despite the potential of RNAi for human therapeutics and crop bio-protection, analytical platforms to quantitatively validate the slicing …

ru, de, ch (code pays fourni par la source)

Ein Cocktail gegen Giftschlangen

Michael Lienhard Schmitz

Synergistic targeting of cancer cells through simultaneous inhibition of key metabolic enzymes

Jan Dreute, Julia Stengel, Jonas Becher, David van den Borre et autres

As cancer cell specific rewiring of metabolic networks creates potential therapeutic opportunities, we conducted a synthetic lethal screen utilizing inhibitors of metabolic pathways. Simultaneous administration of (R)-GNE-140 and BMS-986205 (Linrodostat) preferentially halted proliferation of ovarian cancer cells, but not of their non-oncogenically …

de, us (code pays fourni par la source)

Insights from human NF-κB knockouts

Maximilian Pfisterer, Jan Dreute, Michael Lienhard Schmitz

The well-studied NF-κB signaling system is a key mediator of the inflammatory response. Large-scale sequencing studies in humans now allow initial insights into non-essential human genes in which both alleles carry mutations that prevent protein expression or function. Here, we compiled the …

de (code pays fourni par la source)

Synergistic targeting of cancer cells through simultaneous inhibition of key metabolic enzymes

Jan Dreute, Julia Stengel, Jonas Becher, David R. van den Borre et autres

Abstract As cancer cell specific rewiring of metabolic networks creates potential therapeutic opportunities, we conducted a synthetic lethal screen utilizing inhibitors of metabolic pathways. Simultaneous administration of (R)-GNE-140 and BMS-986205 (Linrodostat) preferentially halted proliferation of ovarian cancer cells, but not of their …

de, us (code pays fourni par la source)

Demonstration of ultra-high dose rate electron irradiation at FLASHlab@PITZ

Xiangkun Li, Z. Amirkhanyan, Anna Grebinyk, Y. Komar et autres

Abstract Objective. The photo injector test facility at DESY in Zeuthen (PITZ) is building up an R&D platform, known as FLASH lab @PITZ, for systematically studying the FLASH effect in cancer treatment with its high-brightness electron beams, which can provide a uniquely …

de (code pays fourni par la source)

The proximity-based protein interactome and regulatory logics of the transcription factor p65 NF-κB/RELA

Lisa Leib, J. Unternaehrer Juli, Liane Jurida, Christin Mayr‐Buro et autres

The protein interactome of p65/RELA, the most active subunit of the transcription factor (TF) NF-κB, has not been previously determined in living cells. Using p65-miniTurbo fusion proteins and biotin tagging, we identify >350 RELA interactors from untreated and IL-1α-stimulated cells, including many …

de (code pays fourni par la source)

Eine neue Funktion für HIF: die Umstellung des Metabolismus im Zellzyklus

Jan Dreute, Maximilian Pfisterer, Michael Lienhard Schmitz

Abstract The family of hypoxia-inducible transcription factors (HIF) is typically activated by low oxygen concentrations. We observed a transient increase of the HIF-1α protein levels during the G1 phase of the cell cycle, designated as G1-HIF. This cell cycle-regulated form of HIF …

de (code pays fourni par la source)

SIAH3 is frequently epigenetically silenced in cancer and regulates mitochondrial metabolism

Verena E. Deutschmeyer, Nico A. Schlaudraff, Sara K. Walesch, Janine Moyer et autres

Of the seven in absentia homologue (SIAH) family, three members have been identified in the human genome. In contrast to the E3 ubiquitin ligase encoding SIAH1 and SIAH2, little is known on the regulation and function of SIAH3 in tumorigenesis. In this …

de (code pays fourni par la source)

Distinct negative-sense RNA viruses induce a common set of transcripts encoding proteins forming an extensive network

Nina Hofmann, Marek Bartkuhn, Stephan Becker, Nadine Biedenkopf et autres

The large group of negative-strand RNA viruses (NSVs) comprises many important pathogens. To identify conserved patterns in host responses, we systematically compared changes in the cellular RNA levels after infection of human hepatoma cells with nine different NSVs of different virulence degrees. …

de (code pays fourni par la source)