Tobias Gräwert

AF4-to-SAXS: expanded characterization of nanoparticles and proteins at the P12 BioSAXS beamline

Stefano Da Vela, Kim Bartels, Daniel Franke, Dmytro Soloviov et autres

Biological small-angle X-ray scattering (SAXS) is a versatile and powerful technique for investigating the structural and biophysical properties of biologically and pharmaceutically relevant macromolecules and nanoparticles. SAXS offers detailed insights into macromolecular composition, size, shape and internal structure, while addressing key aspects …

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Insights into the solution structure of the actin-binding tail domain of metavinculin by small angle X-ray scattering and molecular dynamics simulations

Zeynep A. Oztug Durer, Hande İpek İnce, Zeynep Şevval Düvenci, Emel Timuçin et autres

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New features in ATSAS-4 , a program suite for small-angle scattering data analysis

Daniel Franke, Tobias Gräwert, Dmitri I. Svergun

The ATSAS release 4 series introduces substantial architectural updates and enhanced user-friendly tools for analyzing biological macromolecules and nanostructured objects. In particular, highly requested project support for graphical user interfaces (GUIs) has been implemented. Here the state of the main window of …

Structural basis of CDNF interaction with the UPR regulator GRP78

Melissa Ann Graewert, Maria S. Volkova, Klara Jonasson, Juha A. E. Määttä et autres

Cerebral dopamine neurotrophic factor (CDNF) is an unconventional neurotrophic factor that is a disease-modifying drug candidate for Parkinson’s disease. CDNF has pleiotropic protective effects on stressed cells, but its mechanism of action remains incompletely understood. Here, we use state-of-the-art advanced structural techniques …

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Small-angle x-ray scattering investigation of the integration of free fatty acids in polysorbate 20 micelles

Jörg Ehrit, Tobias Gräwert, Hendrik Göddeke, Petr Valeryevich Konarev et autres

In the originally published version of this article, the following errors appear in Table 1: (1) all values in the fourth column from the left are too large by an order of magnitude, (2) the definition of N mol in the legend …

Small-angle x-ray scattering investigation of the integration of free fatty acids in polysorbate 20 micelles

Jörg Ehrit, Tobias Gräwert, Hendrik Göddeke, Petr Valeryevich Konarev et autres

A critical quality attribute for liquid formulations is the absence of visible particles. Such particles may form upon polysorbate hydrolysis resulting in release of free fatty acids into solution followed by precipitation. Strategies to avoid this effect are of major interest for …

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Mechanism of activation and regulation of deubiquitinase activity in MINDY1 and MINDY2

S.A. Abdul Rehman, Lee A. Armstrong, Sven M. Lange, Yosua Adi Kristariyanto et autres

Of the eight distinct polyubiquitin (polyUb) linkages that can be assembled, the roles of K48-linked polyUb (K48-polyUb) are the most established, with K48-polyUb modified proteins being targeted for degradation. MINDY1 and MINDY2 are members of the MINDY family of deubiquitinases (DUBs) that …

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Mechanism of activation and regulation of Deubiquitinase activity in MINDY1 and MINDY2

S.A. Abdul Rehman, Lee A. Armstrong, Sven M. Lange, Yosua Adi Kristariyanto et autres

ABSTRACT Of the eight distinct polyubiquitin chains that can be assembled, K48-linked ubiquitin is the most well-understood linkage and modification of proteins with K48 chains targets the modified protein for degradation. By removing ubiquitin from substrates or trimming ubiquitin chains, deubiquitinases (DUBs) …

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Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data

Melissa Ann Graewert, Stefano Da Vela, Tobias Gräwert, Dmitry S. Molodenskiy et autres

We describe the updated size-exclusion chromatography small angle X-ray scattering (SEC-SAXS) set-up used at the P12 bioSAXS beam line of the European Molecular Biology Laboratory (EMBL) at the PETRAIII synchrotron, DESY Hamburg (Germany). The addition of size exclusion chromatography (SEC) directly on-line …

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Structural Modeling Using Solution Small-Angle X-ray Scattering (SAXS)

Tobias Gräwert, Dmitri I. Svergun

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Structural Kinetics of MsbA Investigated by Stopped-Flow Time-Resolved Small-Angle X-Ray Scattering

Inokentijs Josts, Yunyun Gao, Diana C. F. Monteiro, Stephan Niebling et autres

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Structure of GTP cyclohydrolase I from Listeria monocytogenes , a potential anti-infective drug target

Sonja Schüssler, Ilka Haase, Markus Perbandt, Boris A. Illarionov et autres

A putative open reading frame encoding GTP cyclohydrolase I from Listeria monocytogenes was expressed in a recombinant Escherichia coli strain. The recombinant protein was purified and was confirmed to convert GTP to dihydroneopterin triphosphate ( K m = 53 µ M ; …

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