Accès ouvert
2018
article
OpenAlex
Roberta Callari, Yvan Meier, Davide Ravasio, Harald Heider
Production of plant metabolites in microbial hosts represents a promising alternative to traditional chemical-based methods. Diterpenoids are compounds with interesting applications as pharmaceuticals, fragrances and biomaterials. Casbene, in particular, serves as a precursor to many complex diterpenoids found in plants from the …
ch
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Accès ouvert
2018
article
OpenAlex
Roberta Callari, David Fischer, Harald Heider, Nora Weber
BACKGROUND: The angelic acid moiety represents an essential modification in many biologically active products. These products are commonly known as angelates and several studies have demonstrated their therapeutic benefits, including anti-inflammatory and anti-cancer effects. However, their availability for use in the development …
dk, ch
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Accès ouvert
2017
article
OpenAlex
Victor Forman, Roberta Callari, Christophe Folly, Harald Heider et autres
The development of medical applications exploiting the broad bioactivities of the diterpene therapeutic triptolide from Tripterygium wilfordii is limited by low extraction yields from the native plant. Furthermore, the extraordinarily high structural complexity prevents an economically attractive enantioselective total synthesis. An alternative …
dk, ch, us
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Accès ouvert
2017
article
OpenAlex
Nora Weber, Anaëlle Hatsch, Ludivine Labagnere, Harald Heider
BACKGROUND: Saccharomyces cerevisiae (baker's yeast) has great potential as a whole-cell biocatalyst for multistep synthesis of various organic molecules. To date, however, few examples exist in the literature of the successful biosynthetic production of chemical compounds, in yeast, that do not exist …
ch
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Accès ouvert
2016
article
OpenAlex
Dan Luo, Roberta Callari, Britta Hamberger, Sileshi Gizachew Wubshet et autres
The seed oil of Euphorbia lathyris L. contains a series of macrocyclic diterpenoids known as Euphorbia factors. They are the current industrial source of ingenol mebutate, which is approved for the treatment of actinic keratosis, a precancerous skin condition. Here, we report …
dk, ch, se
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Accès ouvert
2016
article
OpenAlex
Rosario Vanella, Roberta Callari, Anna A. Weston, Harald Heider et autres
BACKGROUND: Intracellular metabolism of glucocorticoid hormones plays an important role in the pathogenesis of metabolic syndrome and regulates, among many physiological processes, collagen metabolism in skin. At the peripheral level the concentration of active glucocorticoids is mainly regulated by the 11β-hydroxysteroid dehydrogenase …
ch
(code pays fourni par la source)
2001
article
OpenAlex
Harald Heider, Eva Sabine Wintergerst
The role of human macrophage scavenger receptor A1 (SRA1) in the development of atherosclerotic lesions is still scarcely defined. Substituting serine 48 in human SRA1 by an aspartate demonstrated that (1) surface expression of the mutated receptor was 13-fold decreased; (2) the …
ch
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Accès ouvert
2000
article
OpenAlex
Harald Heider, Stefano Brenz Verca, Sandro Rusconi, Reto Asmis
Lipid-mediated transfection was compared to adenoviral-mediated gene transfer in COS-7 cells as well as human monocyte-derived macrophages (HMDM). For this purpose, we monitored enhanced green fluorescent protein (EGFP) expression by fluorescence microscopy and quantified gene transfer by competitive PCR. Transfection of COS-7 …
ch
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1999
article
OpenAlex
Rico Barsacchi, Harald Heider, Jean‐Antoine Girault, Jacopo Meldolesi
The role of the Ca(2+)-activated tyrosine kinase, Pyk2, in the pleiotropic coupling of nerve cell stimulation to the MAP kinase cascade still remains undefined. Using a panel of PC12 clones, one of which was defective in Pyk2, we demonstrate (1) that the …
it, fr
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Accès ouvert
1994
article
OpenAlex
Harald Heider, Corinne Hug, John Milton Lucocq
Mitotic HeLa cells showed an increased phosphorylation activity towards myelin basic protein compared to cells in G1 or S phases. Further investigation using renaturation gels revealed that, in mitotic cell lysates, a protein with an apparent molecular mass of around 40 kDa …
ch, gb
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1992
article
OpenAlex
Jian Liao, Harald Heider, Sun Man-chi, Urs Brodbeck
Acetylcholinesterases (EC 3.1.1.7, AChE) have varying amounts of carbohydrates attached to the core protein. Sequence analysis of the known primary structures gives evidence for several asparagine-linked carbohydrates. From the differences in molecular mass determined on sodium dodecyl sulfate-polyacrylamide gel before and after …
ch
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Accès ouvert
1992
article
OpenAlex
Harald Heider, Urs Brodbeck
Tetrameric detergent-soluble bovine caudate nucleus acetylcholinesterase (AChE) was reduced and alkylated under conditions in which at least 95% of initial activity is retained. This treatment alone did not result in monomerization of AChE, nor did it create a hydrophilic enzyme. However, in …
ch
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