David Fischer

Biochemical characterization of a novel oxidatively stable, halotolerant, and high‐alkaline subtilisin from Alkalihalobacillus okhensis Kh10‐101 T

Fabian Falkenberg, Jade Rahba, David Fischer, Michael Bott et autres

Halophilic and halotolerant microorganisms represent a promising source of salt‐tolerant enzymes suitable for various biotechnological applications where high salt concentrations would otherwise limit enzymatic activity. Considering the current growing enzyme market and the need for more efficient and new biocatalysts, the present …

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Abstract 3499: M1069 as dual A2A/A2B adenosine receptor antagonist counteracts immune-suppressive mechanisms of adenosine and reduces tumor growth in vivo

Rinat Zaynagetdinov, Kai Schiemann, Kalyan C. Nallaparaju, Н. В. Белоусова et autres

Abstract Under physiological conditions, the extracellular concentrations of adenosine are low, however, levels dramatically increase under metabolically stressful conditions, including inflammation and cancer. The regulatory functions of adenosine are mediated through four members of the adenosine receptor family: A1, A2A, A2B, and …

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A homogeneous high-DAR antibody–drug conjugate platform combining THIOMAB antibodies and XTEN polypeptides

Neelie T. Zacharias, Vladimir N. Podust, Kimberly K. Kajihara, Douglas D. Leipold et autres

Efficiency of targeted cell delivery of small molecules was enhanced in cells and animals via a novel well-defined bioconjugation platform combining site-specific antibody conjugation and XTEN polypeptides to enable high payload loading.

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A homogeneous high-DAR antibody-drug conjugate platform combining THIOMABTM antibodies and XTEN polypeptides

Neelie T. Zacharias, Vladimir N. Podust, Kimberly K. Kajihara, Douglas D. Leipold et autres

Abstract Antibody-drug conjugates (ADCs) enable cell-specific delivery of small molecules and are validated anti-cancer therapeutics. One factor limiting ADC advancement and broader application is the drug-to-antibody ratio (DAR), which dictates the number of payloads that can be delivered per antibody. With few …

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Astrocyte glutathione maintains endothelial barrier stability

Sheng‐Fu Huang, Alaa Othman, Alexey Koshkin, Sabrina Fischer et autres

Blood-brain barrier (BBB) impairment clearly accelerates brain disease progression. As ways to prevent injury-induced barrier dysfunction remain elusive, better understanding of how BBB cells interact and modulate barrier integrity is needed. Our metabolomic profiling study showed that cell-specific adaptation to injury correlates …

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Cell-specific metabolomic responses to injury: novel insights into blood-brain barrier modulation

Sheng‐Fu Huang, Sabrina Fischer, Alexey Koshkin, Endre Laczkó et autres

On one hand blood-brain barrier (BBB) disturbance aggravates disease progression, on the other it prevents drug access and impedes therapeutic efficacy. Effective ways to modulate barrier function and resolve these issues are sorely needed. Convinced that better understanding of cell-oriented BBB responses …

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Data on charge separation of bispecific and mispaired IgGs using native charge-variant mass spectrometry

Wilson Phung, Guanghui Han, Stéphanie G. I. Polderdijk, Michael Dillon et autres

The data supplied in this work are related to the research article entitled "Characterization of Bispecific and Mispaired IgGs by Native Charge-Variant Mass Spectrometry" (Phung et al., 2019). This data article describes a powerful analytical platform using native weak cation exchange chromatography …

Characterization of bispecific and mispaired IgGs by native charge-variant mass spectrometry

Wilson Phung, Guanghui Han, Stéphanie G. I. Polderdijk, Michael Dillon et autres

Correction to: Indirect and direct routes to C-glycosylated flavones in Saccharomyces cerevisiae

Katherina García Vanegas, Arésu Bondrup Larsen, Michael Eichenberger, David Fischer et autres

Upon publication of this article [1], it was brought to our attention that revised Fig. 1 supplied by the author during proof correction was unfortunately not presented in the original version of the article. The revised Fig. 1 is given in this …

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Indirect and direct routes to C-glycosylated flavones in Saccharomyces cerevisiae

Katherina García Vanegas, Arésu Bondrup Larsen, Michael Eichenberger, David Fischer et autres

BACKGROUND: C-glycosylated flavones have recently attracted increased attention due to their possible benefits in human health. These biologically active compounds are part of the human diet, and the C-linkage makes them more resistant to hydrolysis and degradation than O-glycosides. In contrast to …

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Biosynthesis of angelyl-CoA in Saccharomyces cerevisiae

Roberta Callari, David Fischer, Harald Heider, Nora Weber

BACKGROUND: The angelic acid moiety represents an essential modification in many biologically active products. These products are commonly known as angelates and several studies have demonstrated their therapeutic benefits, including anti-inflammatory and anti-cancer effects. However, their availability for use in the development …

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De novo biosynthesis of anthocyanins in Saccharomyces cerevisiae

Michael Eichenberger, Anders Hansson, David Fischer, Lara Dürr et autres

Anthocyanins (ACNs) are plant secondary metabolites responsible for most of the red, purple and blue colors of flowers, fruits and vegetables. They are increasingly used in the food and beverage industry as natural alternative to artificial colorants. Production of these compounds by …

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