Lee B Barrett

Discovery of Na v 1.7 Inhibitors through the Screening of Marine Natural Product Extracts

Adetola H. Adewole, Bhuwan Khatri Chhetri, Ghada M. Abdelwahab, Riya Bhanushali et autres

High Resolution Image Download MS PowerPoint Slide Automated high-throughput screening of a prefractionated extract library of marine macroorganisms identified 239 hits (hit rate 2.5%), including a marine algal extract that blocked the Na v 1.7 channel in a fluorescent-based flux assay. Bioactivity-guided …

us, bg (code pays fourni par la source)

A machine learning tool with light-based image analysis for automatic classification of 3D pain behaviors

Omer Barkai, Bruna Lenfers Turnes, Maryam Arab, David A. Yarmolinsky et autres

Detailed assessment of pain-related behaviors in animals is essential for both exploring pain mechanisms and evaluating analgesic efficacy. While pose estimation tools have advanced automated behavior analysis, current existing algorithms often do not account for an animal's body-part contact intensity with-and distance …

us (code pays fourni par la source)

Non-muscle myosin II inhibition at the site of axon injury increases axon regeneration

Keunjung Heo, Tammy Szu‐Yu Ho, Xiangsunze Zeng, Bruna Lenfers Turnes et autres

Motor axon regeneration following peripheral nerve injury is critical for motor recovery but therapeutic interventions enhancing this are not available. We conduct a phenotypic screen on human motor neurons and identified blebbistatin, a non-muscle myosin II inhibitor, as the most effective neurite …

us (code pays fourni par la source)

Multi-Kinase Inhibition of STE20 Kinases is Neuroprotective Against Chemotherapy-Induced Axon Degeneration

Veselina Petrova, Caitlin E. Mills, Clemens B. Hug, Aysel Cetinkaya‐Fisgin et autres

us, mx (code pays fourni par la source)

ARBEL: A Machine Learning Tool with Light-Based Image Analysis for Automatic Classification of 3D Pain Behaviors

Omer Barkai, Biyao Zhang, Bruna Lenfers Turnes, Maryam Arab et autres

A detailed analysis of pain-related behaviors in rodents is essential for exploring both the mechanisms of pain and evaluating analgesic efficacy. With the advancement of pose-estimation tools, automatic single-camera video animal behavior pipelines are growing and integrating rapidly into quantitative behavioral research. …

us (code pays fourni par la source)

Non-muscle Myosin Ii Inhibition at the Site of Axon Injury Increases Axon Regeneration

Clifford J. Woolf, Keunjung Heo, Tammy Szu‐Yu Ho, Xiangsunze Zeng et autres

us (code pays fourni par la source)

Identification of novel neuroprotectants against vincristine-induced neurotoxicity in iPSC-derived neurons

Veselina Petrova, Andrew R. Snavely, Jennifer Splaine, Shannon Zhen et autres

Chemotherapy-induced peripheral neuropathy (CIPN) is a disabling side effect of cancer chemotherapy that can often limit treatment options for cancer patients or have life-long neurodegenerative consequences that reduce the patient's quality of life. CIPN is caused by the detrimental actions of various …

us (code pays fourni par la source)

Identification of novel neuroprotectants against vincristine-induced neurotoxicity in iPSC-derived neurons

Veselina Petrova, Andrew R. Snavely, Jennifer Splaine, Shannon Zhen et autres

us (code pays fourni par la source)

High-content screening identifies a small molecule that restores AP-4-dependent protein trafficking in neuronal models of AP-4-associated hereditary spastic paraplegia

Afshin Saffari, Barbara Brechmann, Cedric Böger, Wardiya Afshar Saber et autres

Unbiased phenotypic screens in patient-relevant disease models offer the potential to detect therapeutic targets for rare diseases. In this study, we developed a high-throughput screening assay to identify molecules that correct aberrant protein trafficking in adapter protein complex 4 (AP-4) deficiency, a …

us, de, gb (code pays fourni par la source)

High-Content Screening Identifies A Small Molecule That Restores AP-4-dependent Protein Trafficking In Neuronal Models Of AP-4-Associated Hereditary Spastic Paraplegia

Afshin Saffari, Barbara Brechmann, Cedric Böger, Wardiya Afshar Saber et autres

Supplementary data of the manuscript: High-Content Small Molecule Screen Identifies a Novel Compound That Restores AP-4-Dependent Protein Trafficking in Neuronal Models of AP-4-Associated Hereditary Spastic Paraplegia

High-Content Small Molecule Screen Identifies a Novel Compound That Restores AP-4-Dependent Protein Trafficking in Neuronal Models of AP-4-Associated Hereditary Spastic Paraplegia

Afshin Saffari, Barbara Brechmann, Cedric Boeger, Wardiya Afshar Saber et autres

Abstract Unbiased phenotypic screens in patient-relevant disease models offer the potential to detect novel therapeutic targets for rare diseases. In this study, we developed a high-throughput screening assay to identify molecules that correct aberrant protein trafficking in adaptor protein complex 4 (AP-4) …

us, de (code pays fourni par la source)

Phenotypic drug screen uncovers the metabolic GCH1/BH4 pathway as key regulator of EGFR/KRAS-mediated neuropathic pain and lung cancer

Shane J. F. Cronin, Shuan Rao, Miguel Angel Tejada, Bruna Lenfers Turnes et autres

Increased tetrahydrobiopterin (BH4) generated in injured sensory neurons contributes to increased pain sensitivity and its persistence. GTP cyclohydrolase 1 (GCH1) is the rate-limiting enzyme in the de novo BH4 synthetic pathway, and human single-nucleotide polymorphism studies, together with mouse genetic modeling, have …

at, us, gb, br, ca (code pays fourni par la source)