Heather A. Bruce

Tetravalent antibodies are more potent and efficacious erythropoiesis‐stimulating agents than erythropoietin in vivo

Jarrett Adams, Levi L. Blazer, Jacky Chung, Minoo Karimi et autres

Recent studies have shown that tetravalent antibodies are potent and efficacious agonists of the erythropoietin (EPO) receptor (EPOR) both in vitro and in vivo. To identify antibody-based erythropoiesis-stimulating agents (ESAs) with therapeutic potential, we evaluated various tetravalent antibody formats for EPOR agonism …

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Synthetic antibodies targeting EphA2 induce diverse signaling‐competent clusters with differential activation

Jarrett Adams, Heather A. Bruce, Suryasree Subramania, Lynda Ploder et autres

The receptor tyrosine kinase EphA2 interacts with ephrin (Efn) ligands to mediate bi-directional signals that drive cellular sorting processes during tissue development. In the context of various cancers, EphA2 can also drive invasive metastatic disease and represents an important target for cancer …

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Antigen‐binding fragments with improved crystal lattice packing and enhanced conformational flexibility at the elbow region as crystallization chaperones

Heather A. Bruce, A.U. Singer, Levi L. Blazer, Khanh Luu et autres

It has been shown previously that a set of three modifications-termed S1, Crystal Kappa, and elbow-act synergistically to improve the crystallizability of an antigen-binding fragment (Fab) framework. Here, we prepared a phage-displayed library and performed crystallization screenings to identify additional substitutions-located near …

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Engineered Antigen-Binding Fragments for Enhanced Crystallisation of Antibody:Antigen Complexes

Heather A. Bruce, A.U. Singer, E.V. Filippova, Levi L. Blazer et autres

ABSTRACT The atomic-resolution structural information that X-ray crystallography can provide on the binding interface between a Fab and its cognate antigen is highly valuable for understanding the mechanism of interaction. However, many Fab:antigen complexes are recalcitrant to crystallisation, making the endeavour a …

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F27. Heritability of Gamma Butyric Acid: A Mega-Press With Macromolecule Suppression Study

Heather A. Bruce, Laura M. Rowland, Peter Kochunov, Elliot Hong

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Analysis of the natively unstructured RNA/protein-recognition core in the Escherichia coli RNA degradosome and its interactions with regulatory RNA/Hfq complexes

Heather A. Bruce, Dijun Du, Dijana Matak‐Vinković, Katarzyna Bandyra et autres

The RNA degradosome is a multi-enzyme assembly that plays a central role in the RNA metabolism of Escherichia coli and numerous other bacterial species including pathogens. At the core of the assembly is the endoribonuclease RNase E, one of the largest E. …

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Recognition of the small regulatory RNA RydC by the bacterial Hfq protein

Daniela Dimastrogiovanni, Kathrin S. Fröhlich, Katarzyna Bandyra, Heather A. Bruce et autres

Bacterial small RNAs (sRNAs) are key elements of regulatory networks that modulate gene expression. The sRNA RydC of Salmonella sp. and Escherichia coli is an example of this class of riboregulators. Like many other sRNAs, RydC bears a 'seed' region that recognises …

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Author response: Recognition of the small regulatory RNA RydC by the bacterial Hfq protein

Daniela Dimastrogiovanni, Kathrin S. Fröhlich, Katarzyna Bandyra, Heather A. Bruce et autres

A crucial step in the production of proteins is the translation of messenger RNA molecules. Other RNA molecules called small RNAs are also involved in this process: these small RNAs bind to the messenger RNA molecules to either increase or decrease the …

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Structures of a γ-aminobutyrate (GABA) transaminase from thes-triazine-degrading organismArthrobacter aurescensTC1 in complex with PLP and with its external aldimine PLP–GABA adduct

Heather A. Bruce, Juan Mangas‐Sánchez, Charlotte Leese, Jennifer Hopwood et autres

Two complex structures of the γ-aminobutyrate (GABA) transaminase A1R958 from Arthrobacter aurescens TC1 are presented. The first, determined to a resolution of 2.80 Å, features the internal aldimine formed by reaction between the ℇ-amino group of Lys295 and the cofactor pyridoxal phosphate …

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GABA-transaminase A1R958 in complex with external aldimine PLP-GABA adduct

Heather A. Bruce, Juan Mangas‐Sánchez, Sam Hart, J.P. Turkenburg et autres

Structure of GABA-transaminase A1R958 from Arthrobacter aurescens in complex with PLP

Heather A. Bruce, Juan Mangas‐Sánchez, Sam Hart, J.P. Turkenburg et autres

Long tandem repeats as a form of genomic copy number variation: structure and length polymorphism of a chromosome 5p repeat in control and schizophrenia populations

Heather A. Bruce, Nancy A. Sachs, Dobrila D. Rudnicki, Stephanie G. Lin et autres

OBJECTIVES: Genomic copy number variations (CNVs) are a major form of variation in the human genome and play an etiologic role in several neuropsychiatric diseases. Tandem repeats, particularly with long (>50 bp) repeat units, are a relatively common yet underexplored type of …

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