Mingji Li

Isolation of full-length IgG antibodies from combinatorial libraries expressed in the cytoplasm of Escherichia coli

Michael-Paul Robinson, Jinjoo Jung, Natalia Lopez‐Barbosa, Matthew Wook Chang et autres

Here we describe a facile and robust genetic selection for isolating full-length IgG antibodies from combinatorial libraries expressed in the cytoplasm of redox-engineered Escherichia coli cells. The method is based on the transport of a bifunctional substrate comprised of an antigen fused …

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The elevation of salinity above 1% deteriorated nitrification performance and reshaped nitrifier community of an MBR: An often overlooked factor in the treatment of high-strength ammonium wastewater

Jie Zou, Kaoming Zhang, Sifang Wang, Mingji Li et autres

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A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans

Thapakorn Jaroentomeechai, Yong Hyun Kwon, Yiwen Liu, Olivia Young et autres

The ability to reconstitute natural glycosylation pathways or prototype entirely new ones from scratch is hampered by the limited availability of functional glycoenzymes, many of which are membrane proteins that fail to express in heterologous hosts. Here, we describe a strategy for …

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A Predict Method of Measuring Equipment Operation Performance Based on Improved Local Weighted Partial Least Squares

Mingji Li, Ning Cao, Hao Lu, Fan Gao

In view of the fact that the operation data of metering equipment in the power system has strong nonlinearity and immediacy, traditional methods cannot effectively model, predict and analyze. This paper proposes an improved local weighted partial least squares algorithm (K-MLWPLS) based …

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Engineering a Supersecreting Strain of Escherichia coli by Directed Coevolution of the Multiprotein Tat Translocation Machinery

May N. Taw, Mingji Li, Daniel Kim, Mark A. Rocco et autres

remains one of the preferred hosts for biotechnological protein production due to its robust growth in culture and ease of genetic manipulation. It is often desirable to export recombinant proteins into the periplasmic space for reasons related to proper disulfide bond formation, …

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Shotgun scanning glycomutagenesis: A simple and efficient strategy for constructing and characterizing neoglycoproteins

Mingji Li, Xiaolu Zheng, Sudhanshu Shanker, Thapakorn Jaroentomeechai et autres

Significance Asparagine-linked ( N -linked) protein glycosylation—the covalent attachment of complex sugars to the nitrogen atom in asparagine side chains—is the most widespread posttranslational modification to proteins and also the most complex. N- glycosylation affects a significant number of cellular proteins and …

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Engineering Single Pan-Specific Ubiquibodies for Targeted Degradation of All Forms of Endogenous ERK Protein Kinase

Erin A. Stephens, Morgan B. Ludwicki, Bunyarit Meksiriporn, Mingji Li et autres

Ubiquibodies (uAbs) are a customizable proteome editing technology that utilizes E3 ubiquitin ligases genetically fused to synthetic binding proteins to steer otherwise stable proteins of interest (POIs) to the 26S proteasome for degradation. The ability of engineered uAbs to accelerate the turnover …

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Engineering a super-secreting strain of Escherichia coli by directed co-evolution of the multiprotein Tat translocation machinery

May N. Taw, Mingji Li, Daniel Kim, Mark A. Rocco et autres

Abstract Escherichia coli remains one of the preferred hosts for biotechnological protein production due to its robust growth in culture and ease of genetic manipulation. It is often desirable to export recombinant proteins into the periplasmic space for reasons related to proper …

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Engineering single pan-specific ubiquibodies for targeted degradation of all forms of endogenous ERK protein kinase

Erin A. Stephens, Morgan B. Ludwicki, Bunyarit Meksiriporn, Mingji Li et autres

Abstract Ubiquibodies (uAbs) are a customizable proteome editing technology that utilizes E3 ubiquitin ligases genetically fused to synthetic binding proteins to steer otherwise stable proteins of interest (POIs) to the proteasome for degradation. The ability of engineered uAbs to accelerate the turnover …

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Respiratory mortality associated with ozone in China: A systematic review and meta-analysis

Yifan Zhang, Yuxia Ma, Fengliu Feng, Bowen Cheng et autres

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Cell-Free Synthetic Glycobiology: Designing and Engineering Glycomolecules Outside of Living Cells

Thapakorn Jaroentomeechai, May N. Taw, Mingji Li, Alicia K. Aquino et autres

Glycans and glycosylated biomolecules are directly involved in almost every biological process as well as the etiology of most major diseases. Hence, glycoscience knowledge is essential to efforts aimed at addressing fundamental challenges in understanding and improving human health, protecting the environment …

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Shotgun scanning glycomutagenesis: a simple and efficient strategy for constructing and characterizing neoglycoproteins

Mingji Li, Xiaolu Zheng, Sudhanshu Shanker, Thapakorn Jaroentomeechai et autres

Abstract As a common protein modification, asparagine-linked ( N- linked) glycosylation has the capacity to greatly influence the biological and biophysical properties of proteins. However, the routine use of glycosylation at naïve sites as a strategy for engineering proteins with advantageous properties …

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