Accès ouvert
2021
article
OpenAlex
Erin A. Stephens, Morgan B. Ludwicki, Bunyarit Meksiriporn, Mingji Li et autres
Ubiquibodies (uAbs) are a customizable proteome editing technology that utilizes E3 ubiquitin ligases genetically fused to synthetic binding proteins to steer otherwise stable proteins of interest (POIs) to the 26S proteasome for degradation. The ability of engineered uAbs to accelerate the turnover …
us, ch
(code pays fourni par la source)
Accès ouvert
2021
preprint
OpenAlex
Erin A. Stephens, Morgan B. Ludwicki, Bunyarit Meksiriporn, Mingji Li et autres
Abstract Ubiquibodies (uAbs) are a customizable proteome editing technology that utilizes E3 ubiquitin ligases genetically fused to synthetic binding proteins to steer otherwise stable proteins of interest (POIs) to the proteasome for degradation. The ability of engineered uAbs to accelerate the turnover …
us, ch
(code pays fourni par la source)
2019
article
OpenAlex
Natalia Lopez‐Barbosa, Morgan B. Ludwicki, Matthew P. DeLisa
Abstract Protein silencing is an important aspect of both scientific investigation of native protein function and therapeutic targeting of aberrant protein activity. Many techniques for silencing proteins at the DNA or RNA level exist such as CRISPR, RNAi, or TALEN. Cellular proteins …
us
(code pays fourni par la source)
Accès ouvert
2019
article
OpenAlex
Morgan B. Ludwicki, Jiahe Li, Erin A. Stephens, Richard W. Roberts et autres
High Resolution Image Download MS PowerPoint Slide Manipulation of the ubiquitin-proteasome pathway to achieve targeted silencing of cellular proteins has emerged as a reliable and customizable strategy for remodeling the mammalian proteome. One such approach involves engineering bifunctional proteins called ubiquibodies that …
us
(code pays fourni par la source)
Accès ouvert
2019
article
OpenAlex
Bunyarit Meksiriporn, Morgan B. Ludwicki, Erin A. Stephens, Allen Yujie Jiang et autres
There is an urgent need for affinity reagents that target phospho-modified sites on individual proteins; however, generating such reagents remains a significant challenge. Here, we describe a genetic selection strategy for routine laboratory isolation of phospho-specific designed ankyrin repeat proteins (DARPins) by …
us, th, ch
(code pays fourni par la source)