Oscar E. Ruiz

Bone phenotype in a mouse model of classical Ehlers-Danlos syndrome with Col5a1 haploinsufficiency

Keren Machol, Catherine Glauber Ambrose, Deidre N Meyers, Nadja Fratzl‐Zelman et autres

Type V collagen is a key regulator in the formation of type I collagen fibrils in connective tissues including skin, tendons, and bones. Abnormalities in type V collagen cause classical Ehlers Danlos syndrome (EDS), a connective tissue disorder with primarily skin and …

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Advanced tissue clearing and three-dimensional imaging approaches to visualize neural innervation in the rat knee joints

Mairobys Socorro, Janak Gaire, Juliane Rolim de Lavôr, Yenisel Cruz-Almeida et autres

Tissue clearing techniques, combined with immunolabeling and three-dimensional (3-D) high-resolution imaging, have emerged as powerful tools for mapping the architecture of nerves that supply a specific tissue. However, despite significant advances in these techniques, visualizing nerve fibers within joint structures remains a …

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MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible cloning toolbox for use in vertebrate model systems

William B. Gillespie, Yuwen Zhang, Oscar E. Ruiz, Juan Cerda et autres

Here, we present MultiSite Assembly of Gateway Induced Clones (MAGIC), which leverages Gateway-based recombinatorial cloning technology for rapid, modular assembly of plasmids to facilitate transgenesis in cells and vertebrate animal models. The MAGIC collection of plasmids spans a range of in vitro …

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A guide to developing harmonized research workflows in a team science context

Oscar E. Ruiz, Joost Wagenaar, Bella Y. Mehta, Ilias Ziogas et autres

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Translation of single channel electro encephalic signals into limb motion

Oscar E. Ruiz, Lindolpho Oliveira de Araújo, Fabiano Bhering

Neural prostheses (NPs) are devices that can translate brainwaves into motion. The non-invasive multi-channel headset used in the study of Brain–Computer Interface (BCI) systems for the development of NPs, presents high resolution in data collection, but also presents high computing expenses and …

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Combined structural and molecular zebrafish embryonic imaging on ethanol exposure effects during vasculature development

Md. Mobarak Karim, Leah A. Lewis, Christian Zevallos-Delgado, Oscar E. Ruiz et autres

Mothers who consume ethanol during pregnancy expose their fetus to ethanol, leading to a range of birth defects. These include craniofacial defects, intellectual impairments, sensorimotor disabilities, and organ deformations, which are collectively known as fetal alcohol spectrum disorder (FASD). Zebrafish are a …

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ATRX silences Cartpt expression in osteoblastic cells during skeletal development

Yi‐Ting Chen, Ming-Ming Jiang, Carolina Leynes, Mary Adeyeye et autres

ATP-dependent chromatin remodeling protein ATRX is an essential regulator involved in maintenance of DNA structure and chromatin state and regulation of gene expression during development. ATRX was originally identified as the monogenic cause of X-linked α-thalassemia mental retardation (ATR-X) syndrome. Affected individuals …

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Multisite Assembly of Gateway Induced Clones (MAGIC): a flexible cloning toolbox with diverse applications in vertebrate model systems

William Gillespie, Yuwen Zhang, Oscar E. Ruiz, Juan Cerda et autres

ABSTRACT Here we present the Multisite Assembly of Gateway Induced Clones (MAGIC) system, which harnesses site-specific recombination-based cloning via Gateway technology for rapid, modular assembly of between 1 and 3 “Entry” vector components, all into a fourth, standard high copy “Destination” plasmid …

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Imaging zebrafish embryonic development with multimodal optical coherence tomography and light-sheet fluorescence microscopy

Md. Mobarak Karim, Ruijiao Sun, Oscar E. Ruiz, Leah A. Lewis et autres

Zebrafish are a widely used developmental model because of their transparent embryos and external development. These distinctive characteristics provide valuable insights into embryonic development. Optical coherence tomography (OCT) offers label-free structural imaging and has emerged as a preferred tool for embryonic imaging. …

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Facial analytics based on a coordinate extrapolation system (zFACE) for morphometric phenotyping of developing zebrafish

Lorena Maili, Oscar E. Ruiz, Philip H. Kahan, Frankie Chiu et autres

Facial development requires a complex and coordinated series of cellular events that, when perturbed, can lead to structural birth defects. A quantitative approach to quickly assess morphological changes could address how genetic or environmental inputs lead to differences in facial shape and …

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zFACE: Facial Analytics from a Coordinate Extrapolation System for Developing Zebrafish

Lorena Maili, Oscar E. Ruiz, Philip H. Kahan, Stephen T. Larson et autres

ABSTRACT Facial development requires a complex and coordinated series of cellular events, that when perturbed, can lead to structural birth defects. A standardized quantitative approach to quickly assess morphological changes could address how genetic or environmental inputs lead to differences in facial …

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Polygenic risk impactsPDGFRAmutation penetrance in non-syndromic cleft lip and palate

Yao Yu, Rolando Alvarado, Lauren E. Petty, Ryan J. Bohlender et autres

Non-syndromic cleft lip with or without cleft palate (NSCL/P) is a common, severe craniofacial malformation that imposes significant medical, psychosocial and financial burdens. NSCL/P is a multifactorial disorder with genetic and environmental factors playing etiologic roles. Currently, only 25% of the genetic …

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