Facial analytics based on a coordinate extrapolation system (zFACE) for morphometric phenotyping of developing zebrafish
Rattachement africain : us. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Facial development requires a complex and coordinated series of cellular events that, when perturbed, can lead to structural birth defects. A quantitative approach to quickly assess morphological changes could address how genetic or environmental inputs lead to differences in facial shape and promote malformations. Here, we report on a method to rapidly analyze craniofacial development in zebrafish embryos using facial analytics based on a coordinate extrapolation system, termed zFACE. Confocal images capture facial structures and morphometric data are quantified based on anatomical landmarks present during development. The quantitative morphometric data can detect phenotypic variation and inform on changes in facial morphology. We applied this approach to show that loss of smarca4a in developing zebrafish leads to craniofacial anomalies, microcephaly and alterations in brain morphology. These changes are characteristic of Coffin-Siris syndrome, a rare human genetic disorder associated with mutations in SMARCA4. Multivariate analysis of zFACE data facilitated the classification of smarca4a mutants based on changes in specific phenotypic characteristics. Together, zFACE provides a way to rapidly and quantitatively assess the impact of genetic alterations on craniofacial development in zebrafish.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Facial analytics based on a coordinate extrapolation system (zFACE) for morphometric phenotyping of developing zebrafish
- Date Crossref
- 01/06/2023
- Éditeur
- The Company of Biologists
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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The University of Texas MD Anderson Cancer Center Genetics and Epigenetics Graduate Program pays non établi dans la noticeÉtablissement de santé
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The University of Texas Health Science Center at Houston pays non établi dans la noticeUniversité ou école supérieure
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McGovern Medical School University of Texas Health Science Center-Houston 1 Pediatric Research Center pays non établi dans la noticeUniversité ou école supérieure
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Pediatric Research Center 1 pays non établi dans la noticeStructure de recherche
Genetics and Epigenetics Graduate Program — The University of Texas MD Anderson Cancer Center, The University of Texas Health Science Center at Houston et Pediatric Research Center — McGovern Medical School University of Texas Health Science Center-Houston 1, avec 1 autre affiliation.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.