Kaiming Cao

Single-bacterium metabolome revealing heterogeneous cellular states in bacterial populations

Liujuan Zhan, Huimin Liu, Wenjiao Chang, Kaiming Cao et autres

Bacterial metabolism is a complex system of interwoven pathways coordinated by an intricate, multilayered regulatory network. Dissecting the metabolism at single bacterial level is highly challenging. Herein, we greatly increase the sensitivity of the bacterial measurement with our home-made single-bacterium metabolome (SinBactM) …

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Broad-spectrum suppression and disassembly of α-synuclein variant aggregates mediated by a ruthenium metallodrug via conserved metal coordination

Shenghu Wang, Weiwei Wu, Lili Sun, Siming Yuan et autres

NAMI-A targets conserved sites of α-synuclein variants via metal coordination, enabling broad-spectrum inhibition of aggregation of α-synuclein pathological variants and overcoming mutational heterogeneity in Parkinson's disease.

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Author response for "Broad-Spectrum Suppression and Disassembly of α-Synuclein Variant Aggregates Mediated by a Ruthenium Metallodrug via Conserved Metal-Coordination"

Shenghu Wang, Weiwei Wu, Lili Sun, Siming Yuan et autres

Metal-induced nanoscale clusterization initiates protein liquid-liquid phase separation

Sijia Xiang, Zhuanghao Hou, Yu Wang, Yang Yang et autres

Liquid-liquid phase separation (LLPS) of biomolecules is a crucial mechanism in regulating cellular functions through dynamic formation of membrane-less organelles. The assembly of nucleation seeds is a key step that triggers LLPS; however, it is challenging to precisely study its assembly mechanism …

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In-Cell Mass Spectrometry and Ultraviolet Photodissociation Navigates the Intracellular Protein Heterogeneity

Shirui Yang, Zhuanghao Hou, Zheyi Liu, Zhixiong Jin et autres

Directly probing the heterogeneous conformations of intracellular proteins within their native cellular environment remains a significant challenge in mass spectrometry (MS). Here, we establish an in-cell MS and ultraviolet photodissociation (UVPD) strategy that directly ejects proteins from living cells into a mass …

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Probing the interaction of cisplatin with calmodulin and its effect upon binding to myosin light-chain kinase

Siming Yuan, S. Wang, Kaiming Cao, Yu Wang et autres

Cisplatin inhibits the interaction between CaM and MLCK, leading to the mobilization of bound Ca 2+ ions and disruption of CaM functions. The findings provide mechanistic insights into the neurotoxicity caused by the drug.

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Identifying and validating angiogenesis-related genes remodeling tumor microenvironment and suppressing immunotherapy response in gastric cancer

Guiyuan Li, Zhe Li, Jing Shen, Xiaolong Ma et autres

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Diverse associations between pancreatic intra-, inter-lobular fat and the development of type 2 diabetes in overweight or obese patients

Lihui Wang, Yinghao Li, Renfeng Li, Jinwen Luan et autres

Pancreatic fat is associated with obesity and type 2 diabetes mellitus (T2DM); however, the relationship between different types of pancreatic fat and diabetes status remains unclear. Therefore, we aimed to determine the potential of different types of pancreatic fat accumulation as a …

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Reactions of Cisplatin with Thioredoxin-1 Regulate Intracellular Redox Homeostasis

S. Wang, Siming Yuan, Hongze Hu, Jiahai Zhang et autres

Cisplatin is a widely used anticancer drug. In addition to inducing DNA damage, increased levels of reactive oxygen species (ROS) play a significant role in cisplatin-induced cell death. Thioredoxin-1 (Trx1), a redox regulatory protein that can scavenge ROS, has been found to …

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Enhancing Tumor Immunotherapy by Multivalent Anti‐PD‐L1 Nanobody Assembled via Ferritin Nanocage

Manman Liu, Duo Jin, Wenxin Yu, Jiaji Yu et autres

Increasing immunotherapy response rate and durability can lead to significant improvements in cancer care. To address this challenge, a novel multivalent immune checkpoint therapeutic platform is constructed through site-specific ligation of anti-PD-L1 nanobody (Nb) on ferritin (Ftn) nanocage. Nb-Ftn blocks PD-1/PD-L1 interaction …

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Mutual promotion of co-condensation of KRAS G-quadruplex and a well-folded protein HMGB1

Yu Wang, Kaiming Cao, Mingxi Zong, Siming Yuan et autres

Liquid-liquid phase separation (LLPS) of G-quadruplex (GQ) is involved in many crucial cellular processes, while the quadruplex-folding and their functions are typically modulated by specific DNA-binding proteins. However, the regulatory mechanism of binding proteins, particularly the well-folded proteins, on the LLPS of …

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Peroxide-Simulating and GSH-Depleting Nanozyme for Enhanced Chemodynamic/Photodynamic Therapy via Induction of Multisource ROS

Gang Liu, Mingyu Liu, Xiujing Li, X Ye et autres

Reactive oxygen species (ROS) generation, using photodynamic therapy (PDT) and chemodynamic therapy (CDT), is a promising strategy for cancer treatment. However, the production of ROS in tumor cells is often limited by hypoxia, insufficient substrates, and high level of ROS scavengers in …

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