Di Wang

高盐度和金属联合暴露对刺参的毒性效应及机制研究

Di Wang

海水淡化作为缓解淡水资源短缺的重要途径，其排放的浓盐水具有高盐度和金属污染的特征，对近海底栖生态系统构成潜在威胁。刺参（Apostichopus japonicus）是我国重要的经济底栖生物，对盐度变化高度敏感，且易通过摄食累积金属。然而，目前关于高盐度与金属联合胁迫对刺参毒性效应的研究仍较为有限。本研究以刺参为对象，设置正常盐度（29 ppt）和高盐度（35 ppt，模拟海水淡化浓盐水排放）两个盐度条件，分别暴露于环境相关浓度的镉（Cd）、铜（Cu）和铁（Fe），暴露时间为16天。通过离子组学、酶活性分析及非靶向代谢组学等技术，研究了高盐度与金属联合胁迫对刺参体壁的毒性效应及机制。主要结果如下： （1）Cd和Cu暴露分别导致其在体壁中的显著富集，而Fe未发生显著富集，这一现象归因于进化保守的铁稳态调控网络。高盐度显著降低了Cu的富集增幅，但对Cd和Fe无显著影响。金属胁迫还扰乱了锰（Mn）、锌（Zn）、钴（Co）等其他金属元素的稳态，且盐度可影响金属胁迫导致的离子稳态紊乱。 （2）双因素方差分析结果显示，盐度对酸性磷酸酶（Acid phosphatase, ACP）、碱性磷酸酶（Alkaline phosphatase, ALP）、丙二醛（Malondialdehyde, MDA）等指标的主效应显著，而金属的主效应相对有限。在正常盐度下，Cd显著提升钠-钾ATP酶（Sodium-aotassium adenosine triphosphatase, Na⁺/K⁺-ATPase）活性，Cu显著抑制超氧化物歧化酶（Superoxide dismutase, SOD）活性；但在高盐度下，这些金属对酶活性的影响效应消失，表明高盐度环境可能通过改变金属形态或抑制酶活性影响刺参的生理响应。 （3）正常盐度下，Cd和Cu胁迫均导致氧化应激标志物视晶酸（Ophthalmic acid, OPH）显著下调，提示细胞优先将底物用于谷胱甘肽合成，导致OPH生成受限；高盐度联合暴露时，OPH不再发生显著变化，推测高盐度本身已构成氧化胁迫，掩盖了金属的毒性效应。正常盐度下，Cd和Cu均导致皂苷类（Periandrin III、Cynarasaponin F等）、酚酸及黄酮类等活性物质显著下调，表明刺参的营养与药用品质可能受到负面影响；高盐度联合暴露时，这些活性物质未出现进一步显著变化，提示高盐度单独作用已抑制皂苷合成，金属未能叠加额外效应。脂质代谢方面，Cd在正常盐度下上调溶血磷脂酰胆碱（Lysophosphatidylcholine, LPC）、溶血磷脂酰丝氨酸（Lysophosphatidylserine, LPS）及神经酰胺（Ceramide, Cer），诱发炎症；高盐度条件下Cd暴露导致醚键连接的甘油二酯Dg(O-18:3/3:0)显著上调，提示可能加剧炎症反应。在代谢通路方面，高盐度不仅能显著扰动嘌呤代谢、酪氨酸代谢及柠檬酸循环，还能调控金属胁迫导致的代谢扰动：使Cd胁迫下的代谢通路从嘌呤和核苷酸代谢转向鞘脂代谢和花生四烯酸代谢；Cu胁迫下的代谢通路从甘油磷脂代谢和牛磺酸代谢转向丙酮酸代谢和柠檬酸循环。 综上，本研究揭示了高盐度与金属联合胁迫下刺参体壁的离子稳态紊乱、酶活性响应及代谢网络重编程，明确了盐度影响金属的毒性效应，并发现刺参关键活性成分（皂苷等）在联合胁迫下显著下调。本研究为海水淡化浓盐水排放的生态风险评估及刺参养殖区合理布局提供了重要科学依据。

DiffLSTM-MTE: A Hybrid LSTM-Diffusion Framework for Virtual iEEG Reconstruction From MEG

Xiangyu Xue, Liankun Ren, Hongyu Zhou, Anqi Dai et autres

Accurate non-invasive characterization of complex brain dynamics, especially aberrant network activity in epilepsy, is crucial yet challenging due to the invasiveness of iEEG and limitations of noninvasive MEG. We introduce DiffLSTM-MTE, a novel deep learning framework that reconstructs virtual iEEG (ViEEG) from …

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Organ-specific role of neutrophil extracellular traps in shaping the postoperative thrombo-inflammatory niche in pancreatic ductal adenocarcinoma: a multi-model machine learning and mechanistic study

Songping Cui, Di Wang, Shaocheng Lyu, Jincan Huang et autres

Background: Neutrophil extracellular traps (NETs) play a crucial role in thrombosis formation and tumor progression. However, the association between NETs and portal vein thrombosis (PVT) is not well understood. Methods: We retrospectively and prospectively developed and validated a predictive model for postoperative …

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Effects of intraoperative higher versus lower positive end-expiratory pressure during one-lung ventilation for thoracic surgery on postoperative pulmonary complications (PROTHOR): a multicentre, international, randomised, controlled, phase 3 trial

Marcelo Gama de Abreu, Ahmed Abdulmomen, Mohamed M. Abu Elyazed, İ̇lknur Hatice AKBUDAK et autres

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Tracing and Mitigating Hallucinations in Multimodal LLMs via Dynamic Attention Localization

Tiancheng Yang, Lin Zhang, Jiaye Lin, Guimin Hu et autres

Multimodal Large Language Models (MLLMs) achieve strong performance on tasks like image captioning and visual question answering, but remain prone to hallucinations, where generated text conflicts with the visual input. Prior work links this partly to insufficient visual attention, but existing attention-based …

Comparison of EEG Burst Suppression and Hemodynamic Effects Between Remimazolam Tosilate and Etomidate During General Anesthesia Induction: A Retrospective Analysis

Ying Cao, Mi Gan, Li Wan, Jun Lü et autres

Background: Remimazolam tosilate, a novel ultra-short-acting benzodiazepine, demonstrates promising safety profiles in clinical settings. While both remimazolam tosilate and etomidate provide hemodynamic stability during anesthesia induction, limited research has directly compared their effects on electroencephalogram (EEG) burst suppression (periods of transient brain …

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Lipocalin-2 regulates astrocyte-oligodendrocyte interaction to drive post-stroke secondary demyelination

Zhenqian Huang, Xiaohao Zhang, Ying Zhao, Mingming Zha et autres

Secondary demyelination worsens outcomes after cerebral infarction, but astrocyte-oligodendrocyte interactions in this process remain unclear. Using distal middle cerebral artery occlusion (dMCAO) in mice, we show that lipocalin-2 (LCN2), partially diffusing from the infarct-adjacent corpus callosum, is transcriptionally and translationally upregulated in …

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Mapping human brain topography to heart rhythms: an SEEG study

Haoxun Yang, Shujia Liu, Guangyuan Jin, Lei Qi et autres

AIMS: The interplay between the heart and brain has been a subject of interest for centuries, as dysfunction in this interaction is implicated in various cardiovascular diseases and neurological disorders. Despite this advancement, there is currently a limited understanding of the mechanisms …

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Structural network-specific effect of extreme capsule stimulation for drug-resistant focal epilepsy

Di Wang, Xiaohua Zhang, Guangyuan Jin, Qiao Wang et autres

Treatment for drug-resistant epilepsy in poor candidates for resection surgeries remains challenging. The prevailing deep brain stimulation of subcortical nuclei is effective but exhibits heterogeneous efficacy and unpredictable side effects. Therefore, the investigation of novel deep brain stimulation targets is of paramount …

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Exploring the mechanism of Yantiao Fang in reducing inflammation in mice with sepsis-induced acute gastrointestinal injury through the Fas/Caspase-8 signaling pathway

Di Wang, Qian Chen, Jianchao Deng, Meng Wang et autres

目的 探讨炎调方调过Fas/Caspase-8信号通路减轻脓毒症急性胃肠损伤小鼠炎症的机制。 方法 取70只BALB/c小鼠随机分为空白组、假手术组和造模小鼠组。通过盲肠结扎穿孔术（cecum ligation and puncture，CLP）构建脓毒症急性胃肠损伤小鼠模型，将造模成功的小鼠随机分为模型组，炎调方低、中、高剂量组，ROCK抑制剂组。苏木素-伊红（HE）染色观察小鼠回肠组织病理学改变；ELISA 法检测各组小鼠血清IL-17、IL-23水平；蛋白印迹法检测回肠组织Fas/Caspase-8信号通路蛋白Fas、FADD和Caspase-8的相对表达；TUNEL染色法检测回肠组织细胞凋亡情况。 结果 与空白组相比，模型组小鼠回肠组织肠黏膜萎缩明显、绒毛排列杂乱，可见断裂、脱落，上皮细胞细胞坏死脱落，炎症细胞浸润明显，小鼠血清中IL-17、IL-23水平升高（P<0.05），回肠组织中Fas、FADD和Caspase-8蛋白的表达升高（P<0.05），肠上皮细胞呈现明显的凋亡现象（P<0.05）。与模型组相比，炎调方组小鼠的回肠组织病理学改变均得到不同程度的改善，血清中IL-17、IL-23水平降低（P<0.05），且回肠组织中Fas、FADD和Caspase-8蛋白的表达降低（P<0.05），肠上皮细胞凋亡减少（P<0.05）。 结论 炎调方可以减轻肠黏膜组织损伤和肠道组织炎症反应，可能是通过调控Fas/Caspase-8信号通路抑制脓毒症急性胃肠损伤小鼠的肠上皮细胞凋亡来发挥作用的。

A novel ship image enhancement method based on fusion of parallel-series stochastic resonance model and multi-scale spiking neural networks

Di Wang, Yang Wang, Z. D. Wang, Jianhong Huang et autres

Ship imaging has an impact on the visual judgment of the steersman and influences ship navigation safety. Traditional image enhancement methods struggle under adverse conditions and fail to account for the physiological mechanisms of human visual perception. Therefore, a novel method was …

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Theta-burst transcranial magnetic stimulation attenuates chronic ischemic demyelination and vascular cognitive impairment in mice

Di Wang, Xiaohao Zhang, Zhenqian Huang, Yunzi Li et autres

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