Leanne C. Duke

Tetraspanin CD9 alters cellular trafficking and endocytosis of tetraspanin CD63, affecting CD63 packaging into small extracellular vesicles

Dirk P. Dittmer, Leanne C. Duke, Robert J. Tomko, Li Sun et autres

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CRISPR-Cas editing technologies for viral-mediated gene therapies of human diseases: Mechanisms, progress, and challenges

Boris Kantor, Leanne C. Duke, Pradeep G. Bhide

The gene therapy landscape has evolved substantially in recent years, beginning with the approval of the first adeno-associated virus-based gene therapy, Luxterna, in 2017. Since then, the US FDA has approved nearly 30 new viral gene therapy programs, with notable examples including …

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Tetraspanin CD9 alters cellular trafficking and endocytosis of tetraspanin CD63, affecting CD63 packaging into small extracellular vesicles

Leanne C. Duke, Allaura S. Cone, Li Sun, Dirk P. Dittmer et autres

Small extracellular vesicles (sEVs) are particles secreted from cells that play vital roles both in normal physiology and in human disease. sEVs are highly enriched in tetraspanin proteins, such as CD9 and CD63, and contain tetraspanin-enriched membrane microdomains involved in loading of …

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Superparamagnetic Iron Oxide Nanoparticle-Labeled Extracellular Vesicles for Magnetic Resonance Imaging of Ischemic Stroke

Shannon Helsper, Xuegang Yuan, Richard Jeske, Jamini Bhagu et autres

Stroke is a leading cause of death and disability worldwide. Extracellular vesicles (EVs) derived from human mesenchymal stem cells (hMSCs) offer a unique and promising alternative to direct cell injection as part of a cell-based therapy for stroke treatment. The development of …

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Extracellular vesicle biogenesis of three‐dimensional human pluripotent stem cells in a novel Vertical‐Wheel bioreactor

Laureana Muok, Li Sun, Colin Esmonde, Hannah Worden et autres

Abstract Extracellular vesicles (EVs) secreted by human‐induced pluripotent stem cells (hiPSCs) have great potential as cell‐free therapies in various diseases, including prevention of blood–brain barrier senescence and stroke. However, there are still challenges in pre‐clinical and clinical use of hiPSC‐EVs due to …

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Upscaling human mesenchymal stromal cell production in a novel vertical-wheel bioreactor enhances extracellular vesicle secretion and cargo profile

Richard Jeske, Chang Liu, Leanne C. Duke, Maria L. Canonicco Castro et autres

Human mesenchymal stromal cells (hMSCs) are mechanically sensitive undergoing phenotypic alterations when subjected to shear stress, cell aggregation, and substrate changes encountered in 3D dynamic bioreactor cultures. However, little is known about how bioreactor microenvironment affects the secretion and cargo profiles of …

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Anticancer and chemosensitization effects of cannabidiol in 2D and 3D cultures of TNBC: involvement of GADD45α, integrin-α5, -β5, -β1, and autophagy

Sunil Kumar Surapaneni, Nilkumar Patel, Li Sun, Nagavendra Kommineni et autres

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Cannabidiol loaded extracellular vesicles sensitize triple-negative breast cancer to doxorubicin in both in-vitro and in vivo models

Nilkumar Patel, Nagavendra Kommineni, Sunil Kumar Surapaneni, Anil Kumar Kalvala et autres

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Zika Virus Hijacks Extracellular Vesicle Tetraspanin Pathways for Cell-to-Cell Transmission

Sara B. York, Li Sun, Allaura S. Cone, Leanne C. Duke et autres

Zika virus is a reemerging infectious disease that spread rapidly across the Caribbean and South America. Infection of pregnant women during the first trimester has been linked to microcephaly, a neurological condition where babies are born with smaller heads due to abnormal …

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Zika virus hijacks extracellular vesicle tetraspanin pathways for cell-to-cell transmission

Sara B. York, Li Sun, Allaura S. Cone, Leanne C. Duke et autres

ABSTRACT Extracellular vesicles (EVs) are membrane-encapsulated structures released by cells which carry signaling factors, proteins and microRNAs that mediate intercellular communication. Accumulating evidence supports an important role of EVs in the progression of neurological conditions and both the spread and pathogenesis of …

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Mesenchymal stem cell-derived extracellular vesicles ameliorate Alzheimer's disease-like phenotypes in a preclinical mouse model

Allaura S. Cone, Xuegang Yuan, Li Sun, Leanne C. Duke et autres

Alzheimer's disease (AD) is an irreversible neurodegenerative disorder that affects more than 44 million people worldwide.Despite the high disease burden, there is no effective treatment for people suffering from AD. Mesenchymal stem cells (MSCs) are multipotent stromal cells that have been widely …

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Epstein-Barr virus LMP1 manipulates the content and functions of extracellular vesicles to enhance metastatic potential of recipient cells

Dingani Nkosi, Li Sun, Leanne C. Duke, David G. Meckes

Extracellular vesicles (EV) mediate intercellular communication events and alterations in normal vesicle content contribute to function and disease initiation or progression. The ability to package a variety of cargo and transmit molecular information between cells renders EVs important mediators of cell-to-cell crosstalk. …

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