Superparamagnetic Iron Oxide Nanoparticle-Labeled Extracellular Vesicles for Magnetic Resonance Imaging of Ischemic Stroke
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Le résumé fourni par la source
Stroke is a leading cause of death and disability worldwide. Extracellular vesicles (EVs) derived from human mesenchymal stem cells (hMSCs) offer a unique and promising alternative to direct cell injection as part of a cell-based therapy for stroke treatment. The development of labeling strategies is essential to identifying the initial biodistribution and clearance of EV-based therapeutics. In this study, hMSC-EVs were labeled with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles for magnetic resonance imaging (MRI). Two methods of preparation were evaluated after EVs were sonicated in the presence of USPIO nanoparticles. The labeled EVs were purified by (1) ultracentrifugation only or (2) an extension of a harvesting approach that employs poly(ethylene glycol) (PEG) to enrich EVs. Following in vitro assessment, labeled EVs were applied to an ischemic stroke model and imaged both immediately and longitudinally using MRI. In vitro assessment showed the EV characteristics after USPIO nanoparticle labeling. The PEG method exhibited a 3.6-fold enhancement in contrast by using an equivalent USPIO concentration of 0.5 mg/mL and equivalent acquisition parameters (TE = 3.5 ms, TR = 5 s) when the dilution factor is considered. Sufficient USPIO nanoparticle labeling was achieved to visualize the initial biodistribution and assess the initial therapeutic potential. Taken together, simultaneous USPIO nanoparticle labeling and EV enrichment with PEG enhanced MRI contrast and improved outcomes with respect to delivery and ischemic stroke recovery.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Superparamagnetic Iron Oxide Nanoparticle-Labeled Extracellular Vesicles for Magnetic Resonance Imaging of Ischemic Stroke
- Date Crossref
- 09/10/2024
- Éditeur
- American Chemical Society (ACS)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Florida State University National High Magnetic Field Laboratory pays non établi dans la noticeUniversité ou école supérieure
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Florida A&M University - Florida State University College of Engineering pays non établi dans la noticeUniversité ou école supérieure
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National High Magnetic Field Laboratory pays non établi dans la noticeStructure de recherche
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FAMU-FSU College of Engineering Chemical & Biomedical Engineering pays non établi dans la noticeUniversité ou école supérieure
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College of Medicine Department of Biomedical Sciences pays non établi dans la noticeUniversité ou école supérieure
National High Magnetic Field Laboratory — Florida State University, Florida A&M University - Florida State University College of Engineering et National High Magnetic Field Laboratory, avec 2 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.