Olga Pletniková

NPAS3-regulated astrocyte mitochondrial bioenergetics is required for cognition

Kateryna Murlanova, Ksenia Novototskaya-Vlasova, Shovgi Huseynov, Olga Pletniková et autres

The basic helix-loop-helix transcription factor neuronal PAS (Per, Arnt, Sim) domain protein 3 (NPAS3) provides transcriptional regulation of metabolic pathways and is highly expressed in astrocytes. NPAS3 variants have been associated with cognitive dysfunction under several neuropsychiatric conditions, but the underlying brain …

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Chromosome X-wide association study in multiple system atrophy identifies sex-differential risk loci

Anindita Ray, Ruth Chia, Memoona Rasheed, Ece Bayram et autres

The human X chromosome accounts for ∼5% of the genome. Despite its size, the role of X-chromosomal variants in human diseases is not well understood, mainly due to its distinct inheritance pattern and the frequent omission of sex chromosomes from genome-wide association …

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UNC-45A drives ATP-independent microtubule severing via defect recognition and repair inhibition, contributing to neurite dystrophy

Asumi Hoshino, Brian T. Castle, Mihir Shetty, Joyce P. Meints et autres

Abstract UNC-45A is the only known ATP-independent microtubule severing protein. Using in vitro reconstitution and TIRF microscopy, we show that, unlike canonical severing enzymes such as katanin, spastin, and fidgetin, which hydrolyze ATP to remove tubulin dimers and promote lattice repair, UNC-45A …

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Dissecting the molecular landscape of Parkinson’s disease and Parkinson’s disease dementia using highly efficient snRNA-seq (HIF-snRNA-seq)

Sung-Ung Kang, Jinhee Park, Shinwon Ha, Dongsan Kim et autres

This study presents a transcriptomic analysis of the cingulate cortex (CING) in Parkinson's disease (PD) and Parkinson's disease dementia (PDD) using a High-efficiency single-nucleus RNA sequencing (HiF-snRNA-seq) protocol optimized for post-mortem brain samples. RNA quality prediction, poly-A tailing, and dCas9-targeted depletion enabled …

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CASP8 intronic expansion identified by poly-glycine-arginine pathology increases Alzheimer’s disease risk

Lien Nguyen, Ramadan Ajredini, Shu Guo, Lisa E. L. Romano et autres

Alzheimer’s disease (AD) affects more than 10% of the population ≥65 y of age, but the underlying biological risks of most AD cases are unclear. We show anti-poly-glycine-arginine (a-polyGR) positive aggregates frequently accumulate in sporadic AD autopsy brains (45/80 cases). We hypothesize …

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Genome sequence analyses identify novel risk loci for multiple system atrophy

Ruth Chia, Anindita Ray, Zalak Shah, Jinhui Ding et autres

Multiple system atrophy (MSA) is an adult-onset, sporadic synucleinopathy characterized by parkinsonism, cerebellar ataxia, and dysautonomia. The genetic architecture of MSA is poorly understood, and treatments are limited to supportive measures. Here, we performed a comprehensive analysis of whole genome sequence data …

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Differential methylation analysis in neuropathologically confirmed dementia with Lewy bodies

Paolo Reho, Sara Sáez-Atiénzar, Paola Ruffo, Sultana Solaiman et autres

Dementia with Lewy bodies (DLB) is a common form of dementia in the elderly population. We performed genome-wide DNA methylation mapping of cerebellar tissue from pathologically confirmed DLB cases and controls to study the epigenetic profile of this understudied disease. After quality …

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Towards a human brain EV atlas: Characteristics of EVs from different brain regions, including small RNA and protein profiles (4/2023)

Yiyao Huang, Tanina Arab, Ashley E. Russell, Emily R. Mallick et autres

This illustration captures the release of extracellular vesicles (EVs) from various regions of the brain, resembling stars scattered across a starry night. These tiny, cell-derived vesicles are vital players in regulating both normal and pathological functions in the central nervous system (CNS). …

Small RNA Profiles of Brain Tissue-Derived Extracellular Vesicles in Alzheimer’s Disease

Yiyao Huang, Tom A. P. Driedonks, Lesley Cheng, Andrey A. Turchinovich et autres

Background: Extracellular vesicles (EVs) and non-coding RNAs (ncRNAs) are emerging contributors to Alzheimer's disease (AD) pathophysiology. Differential abundance of ncRNAs carried by EVs may provide valuable insights into underlying disease mechanisms. Brain tissue-derived EVs (bdEVs) are particularly relevant, as they may offer …

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Toward a human brain extracellular vesicle atlas: Characteristics of extracellular vesicles from different brain regions, including small RNA and protein profiles

Yiyao Huang, Tanina Arab, Ashley E. Russell, Emily R. Mallick et autres

Extracellular vesicles (EVs) are released from different cell types in the central nervous system (CNS) and play roles in regulating physiological and pathological functions. Although brain-derived EVs (bdEVs) have been successfully collected from brain tissue, there is not yet a "bdEV Atlas" …

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Corrigendum for “Mass Spectrometry-Based Proteomics Analysis of Human Substantia Nigra From Parkinson's Disease Patients Identifies Multiple Pathways Potentially Involved in the Disease”

Yura Jang, Olga Pletniková, Juan Carlos Troncoso, Alexander Y. Pantelyat et autres

Mass Spectrometry–Based Proteomics Analysis of Human Substantia Nigra From Parkinson's Disease Patients Identifies Multiple Pathways Potentially Involved in the DiseaseMolecular & Cellular ProteomicsVol. 22Issue 1PreviewIn Brief We conducted an in-depth proteome analysis of human substantia nigra tissues from 15 Parkinson’s disease (PD) …

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Loss of Astrocytic µ Opioid Receptors Exacerbates Aversion Associated with Morphine Withdrawal in Mice: Role of Mitochondrial Respiration

Kateryna Murlanova, Yan Jouroukhin, Ksenia A. Novototskaya-Vlasova, Shovgi Huseynov et autres

Astrocytes express mu/µ opioid receptors, but the function of these receptors remains poorly understood. We evaluated the effects of astrocyte-restricted knockout of µ opioid receptors on reward- and aversion-associated behaviors in mice chronically exposed to morphine. Specifically, one of the floxed alleles …

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