Angélique Sanchez Dafun

PA200 modulates immunoproteasome structure and activity

Dušan Živković, Amélie Bosc-Rosati, Angélique Sanchez Dafun, Fatme Mourtada et autres

The proteasome activator PA200 binds to the catalytic core of both standard proteasome (s20S) and the immunoproteasome (i20S); however, whether PA200 uses the same mechanisms to activate i20S remains unknown. In this work, the cryo-EM structures of singly- and doubly-capped i20S-PA200 complexes, …

fr, gb, de, pl (code pays fourni par la source)

Dominant-negative PSMB10 disrupts immunoproteasome assembly and leads to transient T lymphopenia

Sujal Ghosh, Y Wang, Christoph Schultheiß, Arndt Borkhardt et autres

Immunoproteasomes are specialized multiprotein proteases that degrade intracellular proteins. Their products serve as peptides for human leukocyte antigen class I presentation playing a key role in antiviral defense and self-tolerance. Monoallelic variants in immunoproteasome genes including proteasome subunit β type 10 have …

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Mechanism of Inhibition of the MeTC7 Ligand That Covalently Binds to VDR To Reduce PD-L1 Expression

J.-G. Castel, Carole Peluso‐Iltis, Angélique Sanchez Dafun, Aurora Silvestri et autres

Cancer-associated overexpression of the Vitamin D Receptor (VDR) is associated with good or poor prognosis, depending on the cancer type and stage. Here, we show that VDR is overexpressed in ovarian malignant tissues and upregulates PD-L1 surface expression in tumor cells, enabling …

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Uncovering the Unexpected Role of Proteasome Activator PA200 in Regulating Immunoproteasome Expression and Activity

Marie‐Pierre Bousquet, Dušan Živković, Angélique Sanchez Dafun, Ayse Seda Yazgili et autres

fr, gb, de, pl (code pays fourni par la source)

Revealing the unexpected interplay between the Proteasome Activator PA200 and the immunoproteasome

Dušan Živković, Angélique Sanchez Dafun, Ayse Seda Yazgili, Marijke Jansma et autres

Abstract The proteasome activator PA200 binds to the catalytic core of the proteasome, the 20S, and activates its proteolytic activities. The cellular function of PA200 is poorly understood and appears to be cell type and differentiation specific. Recent evidence suggests that PA200 …

fr, de, pl (code pays fourni par la source)

Elucidating the interplay between the regulatory mechanisms of proteasome complexes with structural mass spectrometry

Angélique Sanchez Dafun

Le protéasome 20S est un complexe multimérique composé de quatre anneaux heptamériques (αββα), responsable de la majorité de la dégradation des protéines cellulaires et d'autres fonctions, y compris la protéostasie, l'inflammation, la réponse immunologique, l'apoptose. Son activité accrue ou diminuée a été …

Elucidating the interplay between the regulatory mechanisms of proteasome complexes with structural mass spectrometry

Angélique Sanchez Dafun

Elucider le dialogue entre les mécanismes de régulations des complexes du protéasome via la spectrométrie de masse structurale Le protéasome 20S est un complexe multimérique composé de quatre anneaux heptamériques (αββα), responsable de la majorité de la dégradation des protéines cellulaires et …

fr (code pays fourni par la source)

Druggable redox pathways against Mycobacterium abscessus in cystic fibrosis patient-derived airway organoids

Stephen Adonai Leon‐Icaza, Salimata Bagayoko, Romain Vergé, Nino Iakobachvili et autres

Mycobacterium abscessus (Mabs) drives life-shortening mortality in cystic fibrosis (CF) patients, primarily because of its resistance to chemotherapeutic agents. To date, our knowledge on the host and bacterial determinants driving Mabs pathology in CF patient lung remains rudimentary. Here, we used human …

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Establishing 20S Proteasome Genetic, Translational and Post-Translational Status from Precious Biological and Patient Samples with Top-Down MS

Angélique Sanchez Dafun, Dušan Živković, Stephen Adonai Leon‐Icaza, Sophie Möller et autres

The mammalian 20S catalytic core of the proteasome is made of 14 different subunits (α1-7 and β1-7) but exists as different subtypes depending on the cell type. In immune cells, for instance, constitutive catalytic proteasome subunits can be replaced by the so-called …

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Structural mass spectrometry of membrane proteins

Angélique Sanchez Dafun, Julien Marcoux

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Solution structure of the type I polyketide synthase Pks13 from Mycobacterium tuberculosis

Cécile Bon, Stéphanie Cabantous, Sylviane Julien, Valérie Guillet et autres

BACKGROUND: Type I polyketide synthases (PKSs) are multifunctional enzymes responsible for the biosynthesis of a group of diverse natural compounds with biotechnological and pharmaceutical interest called polyketides. The diversity of polyketides is impressive despite the limited set of catalytic domains used by …

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Proteasome complexes experience profound structural and functional rearrangements throughout mammalian spermatogenesis

Dušan Živković, Angélique Sanchez Dafun, Thomas Menneteau, Adrien Schahl et autres

During spermatogenesis, spermatogonia undergo a series of mitotic and meiotic divisions on their path to spermatozoa. To achieve this, a succession of processes requiring high proteolytic activity are in part orchestrated by the proteasome. The spermatoproteasome (s20S) is specific to the developing …

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