Petra Henklein

Multiplexed Enzyme Activity Microscopy by Semi-quantitative, Ratiometric Enzyme Activity MALDI Mass Spectrometry Imaging

Hamidreza Hojjat, Petra Henklein, Akvile Haeckel, Grit Nebrich et autres

Abstract Enzymes are often tightly regulated and exist in inactive forms. Therefore, rather than mere presence, it is the enzyme’s activity that catalyzes cellular and tissue functions. However, multiplex activity mapping of enzymes in situ is particularly challenging. To address this, we …

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Multiplexed Enzyme Activity Microscopy by Semiquantitative Ratiometric Enzyme Activity MALDI Mass Spectrometry Imaging

Hamidreza Hojjat, Petra Henklein, Akvile Haeckel, Grit Nebrich et autres

This repository contains raw mass spectrometry imaging data associated with the study “Multiplexed Enzyme Activity Microscopy by Semiquantitative, Ratiometric Enzyme Activity MALDI Mass Spectrometry Imaging”. Raw MALDI-MSI datasets are provided in the open imzML format as paired .imzML and .ibd files. Calibration …

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Development and characterization of a membrane-permeant GRASP65-mimetic peptide that inhibits Golgi unlinking and cell cycle progression

Romina Inès Cervigni, Raffaella Bonavita, Maria Luisa Barretta, Daniela Spano et autres

• Golgi ribbon disassembly is crucial for mitosis and plays an important, but not yet understood, role in cancer, aging, and neurodegeneration. • Approaches for block disassembly are lacking. • Phosphorylation of the Golgi protein GRASP65 is crucial for Golgi disassembly. • …

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Corrigendum to CERT1 mutations perturb human development by disrupting sphingolipid homeostasis

Charlotte Gehin, Museer A. Lone, Winston Lee, Laura Capolupo et autres

The authors recently became aware of inadvertently omitted text and a reference citation on page 8. The correct text is provided below. Circular dichroism confirmed that purified CERT 151–309, hereafter referred to as the CERT central core domain (CCD) in reference to …

Corrigendum to CERT1 mutations perturb human development by disrupting sphingolipid homeostasis

Charlotte Gehin, Museer A. Lone, Winston Lee, Laura Capolupo et autres

The α1 integrin cytoplasmic tail interacts with phosphoinositides and interferes with Akt activation

Josephine Labus, Kerstin Tang, Petra Henklein, Ulrike Krüger et autres

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Proteasome inhibition potentiates Kv1.3 potassium channel expression as therapeutic target in drug-sensitive and -resistant human melanoma cells

Clemens Cammann, Jonas Kulla, Lüder Wiebusch, C. Walz et autres

Primary and acquired therapy resistance is a major problem in patients with BRAF-mutant melanomas being treated with BRAF and MEK inhibitors (BRAFI, MEKi). Therefore, development of alternative therapy regimes is still required. In this regard, new drug combinations targeting different pathways to …

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CERT1 mutations perturb human development by disrupting sphingolipid homeostasis

Charlotte Gehin, Museer A. Lone, Winston Lee, Laura Capolupo et autres

Neural differentiation, synaptic transmission, and action potential propagation depend on membrane sphingolipids, whose metabolism is tightly regulated. Mutations in the ceramide transporter CERT (CERT1), which is involved in sphingolipid biosynthesis, are associated with intellectual disability, but the pathogenic mechanism remains obscure. Here, …

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GRASP55 regulates intra‐Golgi localization of glycosylation enzymes to control glycosphingolipid biosynthesis

Prathyush Pothukuchi, Ilenia Agliarulo, Marinella Pirozzi, Riccardo Rizzo et autres

The Golgi apparatus, the main glycosylation station of the cell, consists of a stack of discontinuous cisternae. Glycosylation enzymes are usually concentrated in one or two specific cisternae along the cis‐trans axis of the organelle. How such compartmentalized localization of enzymes is …

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Golgi maturation‐dependent glycoenzyme recycling controls glycosphingolipid biosynthesis and cell growth via GOLPH3

Riccardo Rizzo, Domenico Russo, Kazuo Kurokawa, Pranoy Sahu et autres

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CCDC 1284433: Experimental Crystal Structure Determination

Petra Henklein, B. Costisella, Victor Wray, Tobias Domke et autres

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, …

Autoregulatory circuit regulating basolateral cargo export from the TGN: role of the orphan receptor GPRC5A in PKD signaling and cell polarity

Rosaria Di Martino, Anita Capalbo, Lucia Sticco, Alessandra Varavallo et autres

Abstract The membrane transport apparatus comprises a series of separate membrane bound compartments, or transport stations, that are responsible for the synthesis, processing, transport, sorting and delivery to their final cellular destinations of most transmembrane and soluble lumenal proteins. Over the last …

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