Eli Reuveni

Supplementary Table 5 from Increased RNA and Protein Degradation Is Required for Counteracting Transcriptional Burden and Proteotoxic Stress in Human Aneuploid Cells

Marica Rosaria Ippolito, Johanna Zerbib, Yonatan Eliezer, Eli Reuveni et autres

Details of cell lines, reagents and oligonucleotides used in the study

Supplementary Table 1 from Increased RNA and Protein Degradation Is Required for Counteracting Transcriptional Burden and Proteotoxic Stress in Human Aneuploid Cells

Marica Rosaria Ippolito, Johanna Zerbib, Yonatan Eliezer, Eli Reuveni et autres

Pre-ranked gene set enrichment analysis of differential mRNA expression between the highly-aneuploid clones (SS51, SS111) and the pseudo-diploid clone (SS48).

Supplementary Table 2 from Increased RNA and Protein Degradation Is Required for Counteracting Transcriptional Burden and Proteotoxic Stress in Human Aneuploid Cells

Marica Rosaria Ippolito, Johanna Zerbib, Yonatan Eliezer, Eli Reuveni et autres

Gene set enrichment analysis of differential protein expression between the highly-aneuploid clones (SS51, SS111) and pseudo-diploid clones (SS48, SS31)

Supplementary Table 3 from Increased RNA and Protein Degradation Is Required for Counteracting Transcriptional Burden and Proteotoxic Stress in Human Aneuploid Cells

Marica Rosaria Ippolito, Johanna Zerbib, Yonatan Eliezer, Eli Reuveni et autres

Pre-ranked gene set enrichment analysis of differential mRNA expression between all aneuploid clones (SS6, SS119, SS51, SS111) and the pseudo-diploid clone (SS48)

Supplementary Table 4 from Increased RNA and Protein Degradation Is Required for Counteracting Transcriptional Burden and Proteotoxic Stress in Human Aneuploid Cells

Marica Rosaria Ippolito, Johanna Zerbib, Yonatan Eliezer, Eli Reuveni et autres

PRISM screen results of human cancer cell lines treated with bortezomib, in the absence or presence of reversine

sc-rDSeq: a robust and cost-effective full-length total RNA sequencing method for single cells reveals multilayered heterogeneity in drug-resistant lung cancer cells

Xue Sun, Shir-Liya Dadon, Dena Ennis, Wenpeng Fan et autres

sc-rDSeq is a scalable, full-length total RNA droplet-based technology that captures both polyadenylated and nonpolyadenylated RNAs, including histone RNAs, small and long non-coding RNAs, and enhancer RNAs. It achieves a 10-fold increase in UMIs per cell compared to conventional scRNAseq like 10× …

il (code pays fourni par la source)

Expanding single-cell toolbox with a cost-effective full-length total RNA droplet-based sequencing technology

Xue Sun, Shir-Liya Dadon, Dena Ennis, Muhammad Awawdy et autres

sc-rDSeq is a scalable, full-length total RNA droplet-based technology that captures both polyadenylated and non-polyadenylated RNAs, including histone-RNAs, small and long non-coding RNAs, and enhancer RNAs. It achieves a tenfold increase in UMIs per cell over inDrops while remaining simple and cost-efficient. …

il (code pays fourni par la source)

Data from Increased RNA and Protein Degradation Is Required for Counteracting Transcriptional Burden and Proteotoxic Stress in Human Aneuploid Cells

Marica Rosaria Ippolito, Johanna Zerbib, Yonatan Eliezer, Eli Reuveni et autres

Abstract Aneuploidy results in a stoichiometric imbalance of protein complexes that jeopardizes cellular fitness. Aneuploid cells thus need to compensate for the imbalanced DNA levels by regulating their RNA and protein levels, but the underlying molecular mechanisms remain unknown. In this study, …

Supplementary Table 2 from Increased RNA and Protein Degradation Is Required for Counteracting Transcriptional Burden and Proteotoxic Stress in Human Aneuploid Cells

Marica Rosaria Ippolito, Johanna Zerbib, Yonatan Eliezer, Eli Reuveni et autres

Gene set enrichment analysis of differential protein expression between the highly-aneuploid clones (SS51, SS111) and pseudo-diploid clones (SS48, SS31)

Supplementary Table 3 from Increased RNA and Protein Degradation Is Required for Counteracting Transcriptional Burden and Proteotoxic Stress in Human Aneuploid Cells

Marica Rosaria Ippolito, Johanna Zerbib, Yonatan Eliezer, Eli Reuveni et autres

Pre-ranked gene set enrichment analysis of differential mRNA expression between all aneuploid clones (SS6, SS119, SS51, SS111) and the pseudo-diploid clone (SS48)

Supplementary Figures 1-8 from Increased RNA and Protein Degradation Is Required for Counteracting Transcriptional Burden and Proteotoxic Stress in Human Aneuploid Cells

Marica Rosaria Ippolito, Johanna Zerbib, Yonatan Eliezer, Eli Reuveni et autres

Supplementary Figure 1: Dosage compensation in trisomic cells occurs at both mRNA and protein levels (related to Fig. 1). Supplementary Figure 2: RNA degradation does not confound the results of the differential gene expression analyses (related to Fig. 2). Supplementary Figure 3: …

Supplementary Table 4 from Increased RNA and Protein Degradation Is Required for Counteracting Transcriptional Burden and Proteotoxic Stress in Human Aneuploid Cells

Marica Rosaria Ippolito, Johanna Zerbib, Yonatan Eliezer, Eli Reuveni et autres

PRISM screen results of human cancer cell lines treated with bortezomib, in the absence or presence of reversine