Supplementary Figures 1-8 from Increased RNA and Protein Degradation Is Required for Counteracting Transcriptional Burden and Proteotoxic Stress in Human Aneuploid Cells

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Supplementary Figure 1: Dosage compensation in trisomic cells occurs at both mRNA and protein levels (related to Fig. 1). Supplementary Figure 2: RNA degradation does not confound the results of the differential gene expression analyses (related to Fig. 2). Supplementary Figure 3: Aneuploid clones activate the nonsense-mediated decay (NMD) pathway, and depend on it for downregulating their gene expression (related to Fig. 3). Supplementary Figure 4: Increased dependency on the nonsense-mediated decay pathway is a general feature of aneuploid cells (related to Fig. 3). Supplementary Figure 5: Trisomic clones activate the miRNA-mediated RNA degradation pathway, and depend on it for downregulating their gene expression (related to Fig. 4). Supplementary Figure 6: Aneuploid cells depend on miRNA machinery cofactors PRKRA and TARBP2 (related to Fig. 4). Supplementary Figure 7: Multiple models of aneuploid cells experience proteotoxic stress and attenuate protein translation (related to Fig. 5). Supplementary Figure 8: Multiple models of aneuploid cells activate the proteasome, and depend on its activity for downregulating their protein expression (related to Fig. 6).

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.