Wendy Xolalpa

Role of TRIM24 in the regulation of proteasome-autophagy crosstalk in bortezomib-resistant mantle cell lymphoma

Corentin Bouvier, María Gonzalez-Santamarta, Núria Profitós-Pelejà, Marc Armengol et autres

Abstract Resistance to bortezomib (BTZ) represents a major bottleneck to continue using this proteasome inhibitor in the treatment of mantle cell lymphoma (MCL). In this study, we investigated the mechanisms by which TRIM24 (tripartite motif-containing 24), a ubiquitin ligase enriched in the …

fr, es, dk, pt (code pays fourni par la source)

Change in selectivity of estrogen receptor alpha ligand-binding domain by mutations at residues H524/L525

Emma L. Arévalo-Salina, Takuya Nishigaki, Leticia Olvera, Martín González‐Andrade et autres

The estrogen receptor alpha (ERα) features a large ligand-binding pocket capable of accommodating a variety of conformationally diverse molecules, each eliciting unique cellular responses. This structural plasticity facilitates various conformational changes, exposing different interaction surfaces for cellular proteins, triggering a myriad of …

mx (code pays fourni par la source)

Synthesis of alkyl glucosides catalyzed by immobilized α-amylase from Thermotoga maritima

Wendy Xolalpa, Fidel Ramírez-Amador, Leticia Olvera, Alfonso Miranda‐Molina et autres

Abstract The enzymatic production of alkyl glucosides is limited by the stability of the enzymes in the presence of alcohols. In the present study, we investigated three different supports: Sepharose 4B, crosslinked Sepharose (Fast Flow), and Eupergit C for the immobilization of …

mx, de (code pays fourni par la source)

Breaking Bad Proteins—Discovery Approaches and the Road to Clinic for Degraders

Corentin Bouvier, Rachel Lawrence, Francesca Cavallo, Wendy Xolalpa et autres

Proteolysis-targeting chimeras (PROTACs) describe compounds that bind to and induce degradation of a target by simultaneously binding to a ubiquitin ligase. More generally referred to as bifunctional degraders, PROTACs have led the way in the field of targeted protein degradation (TPD), with …

fr, mx (code pays fourni par la source)

Breaking Bad Proteins – Discovery Approaches and the Road to Clinic for Degraders

Corentin Bouvier, Rachel Lawrence, Francesca Cavallo, Wendy Xolalpa et autres

Proteolysis Targeting Chimeras (PROTACs) describe compounds that bind to and induce degradation of a target by simultaneously binding to a ubiquitin ligase. More generally referred to as bifunctional degraders, PROTACs have led the way in the field of targeted protein degradation (TPD) …

fr, mx (code pays fourni par la source)

The Role of a Loop in the Non-catalytic Domain B on the Hydrolysis/Transglycosylation Specificity of the 4-α-Glucanotransferase from Thermotoga maritima

Alexey Llopiz, Marco A. Ramírez-Martínez, Leticia Olvera, Wendy Xolalpa et autres

The mechanism by which glycoside hydrolases control the reaction specificity through hydrolysis or transglycosylation is a key element embedded in their chemical structures. The determinants of reaction specificity seem to be complex. We looked for structural differences in domain B between the …

mx (code pays fourni par la source)

Constitutive Activation of p62/Sequestosome-1-Mediated Proteaphagy Regulates Proteolysis and Impairs Cell Death in Bortezomib-Resistant Mantle Cell Lymphoma

Grégoire Quinet, Wendy Xolalpa, Diana Reyes-Garau, Núria Profitós-Pelejà et autres

Protein ubiquitylation coordinates crucial cellular events in physiological and pathological conditions. A comparative analysis of the ubiquitin proteome from bortezomib (BTZ)-sensitive and BTZ-resistant mantle cell lymphoma (MCL) revealed an enrichment of the autophagy-lysosome system (ALS) in BTZ-resistant cells. Pharmacological inhibition of autophagy …

fr, es, pt (code pays fourni par la source)

Ubiquitin-chains dynamics and its role regulating crucial cellular processes

María Gonzalez-Santamarta, Corentin Bouvier, Manuel S. Rodríguez, Wendy Xolalpa

fr, mx (code pays fourni par la source)

Deep Eutectic Solvents as New Reaction Media to Produce Alkyl-Glycosides Using Alpha-Amylase from Thermotoga maritima

Alfonso Miranda‐Molina, Wendy Xolalpa, Simon Strompen, Rodrigo Arreola-Barroso et autres

Amy A. The enzyme was almost completely deactivated when assayed in a series of pure DES, but as cosolvents, DES containing alcohols, sugars, and amides as hydrogen-bond donors (HBD) performed best. A choline chloride:urea based DES was further characterized for the alcoholysis …

mx (code pays fourni par la source)

Analysis of defective protein ubiquitylation associated to adriamycin resistant cells

Valérie Lang, Fabienne Aillet, Wendy Xolalpa, Sonia Serna et autres

DNA damage activated by Adriamycin (ADR) promotes ubiquitin–proteasome system-mediated proteolysis by stimulating both the activity of ubiquitylating enzymes and the proteasome. In ADR-resistant breast cancer MCF7 (MCF7ADR) cells, protein ubiquitylation is significantly reduced compared to the parental MCF7 cells. Here, we used …

es, fr, pl (code pays fourni par la source)

Extensive preclinical evaluation of an infliximab biosimilar candidate

Marco Antonio Velasco-Velázquez, Nohemí Salinas-Jazmín, Emiliano Hisaki-Itaya, Luis Enrique Cobos-Puc et autres

mx, cl (code pays fourni par la source)

A comprehensive platform for the analysis of ubiquitin-like protein modifications using in vivo biotinylation

Lucía Pirone, Wendy Xolalpa, Jón Otti Sigurðsson, Juanma Ramírez et autres

Post-translational modification by ubiquitin and ubiquitin-like proteins (UbLs) is fundamental for maintaining protein homeostasis. Efficient isolation of UbL conjugates is hampered by multiple factors, including cost and specificity of reagents, removal of UbLs by proteases, distinguishing UbL conjugates from interactors, and low …

es, dk, fr (code pays fourni par la source)