Takuya Nishigaki

Change in selectivity of estrogen receptor alpha ligand-binding domain by mutations at residues H524/L525

Emma L. Arévalo-Salina, Takuya Nishigaki, Leticia Olvera, Martín González‐Andrade et autres

The estrogen receptor alpha (ERα) features a large ligand-binding pocket capable of accommodating a variety of conformationally diverse molecules, each eliciting unique cellular responses. This structural plasticity facilitates various conformational changes, exposing different interaction surfaces for cellular proteins, triggering a myriad of …

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A FRET-based competitive binding assay using coumestrol and the ligand-binding domain of human estrogen receptor alpha tagged with mTurquoise2 efficiently expressed in E. coli with ethanol

Edith O López-Romero, Emma L. Arévalo-Salina, César Arcos-Hernández, Yoloxochitl Sánchez-Guevara et autres

The estrogen receptor (ER) is a nuclear receptor and one of the most extensively researched targets in the study of endocrine-disrupting chemicals (EDCs). Many biosensors and bioassays for estrogenic EDCs use the ligand-binding domain of human ERα (LBD-hERα) as a biological recognition …

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The sodium–proton exchangers sNHE and NHE1 control plasma membrane hyperpolarization in mouse sperm

Analia G. Novero, Paulina Torres‐Rodríguez, Jose L de La Vega Beltran, Liza J. Schiavi‐Ehrenhaus et autres

Sperm capacitation is a complex process that takes place in the female reproductive tract and empowers mammalian sperm with the competence to fertilize an egg. It consists of an intricate cascade of events that can be mimicked in vitro through incubation in …

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Hyperglycemia adversely affects critical physiological events related to rat sperm capacitation

Hiram Pacheco-Castillo, Erika Elena Zagal-Huerta, Juan José Acevedo‐Fernández, Elizabeth Negrete‐León et autres

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Cytosolic and Acrosomal pH Regulation in Mammalian Sperm

Julio C. Chávez, Gabriela Carrasquel‐Martínez, Sandra Hernández-Garduño, A Matamoros Volante et autres

As in most cells, intracellular pH regulation is fundamental for sperm physiology. Key sperm functions like swimming, maturation, and a unique exocytotic process, the acrosome reaction, necessary for gamete fusion, are deeply influenced by pH. Sperm pH regulation, both intracellularly and within …

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The sodium-proton exchangers sNHE and NHE1 control plasma membrane hyperpolarization in mouse sperm

Analia G. Novero, Paulina Torres‐Rodríguez, Jose L de La Vega Beltran, Liz J Schiavi-Ehrenhaus et autres

ABSTRACT Sperm capacitation, crucial for fertilization, occurs in the female reproductive tract and can be replicated in vitro using a medium rich in bicarbonate, calcium, and albumin. These components trigger the cAMP-PKA signaling cascade, proposed to promote hyperpolarization of the mouse sperm …

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Ca2+ concentrations in mouse sperm mitochondria fluctuate according to the cytosol

Yoloxochitl Sánchez-Guevara, Enrique I. Oliver, Takuya Nishigaki

In brief: Free Ca2+ concentrations within mitochondria ([Ca2+]mt) in mouse spermatozoa were reported in a micromolar range based on the result obtained with Calcium Green 5N. The results obtained with Rhod-2, Fluo-3, and Fluo-5N suggest that the [Ca2+]mt are comparable to the …

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Ion currents through the voltage sensor domain of distinct families of proteins

César Arcos-Hernández, Takuya Nishigaki

Abstract The membrane potential of a cell (Vm) regulates several physiological processes. The voltage sensor domain (VSD) is a region that confers voltage sensitivity to different types of transmembrane proteins such as the following: voltage-gated ion channels, the voltage-sensing phosphatase (Ci-VSP), and …

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How to study a highly toxic protein to bacteria: A case of voltage sensor domain of mouse sperm-specific sodium/proton exchanger

César Arcos-Hernández, Esteban Suárez-Delgado, León D Islas, Francisco Romero et autres

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Hyperpolarization induces cytosolic alkalization of mouse sperm flagellum probably through sperm Na+/H+ exchanger

Sandra Hernández-Garduño, Julio C. Chávez, Arturo Matamoros‐Volante, Yoloxochitl Sánchez-Guevara et autres

In brief: Hyperpolarization of the membrane potential is a crucial step for mammalian sperm maturation. This work demonstrates that this membrane potential change likely activates a sperm-specific sodium/proton exchanger to induce alkalization in mouse sperm flagellum. Abstract: The sperm-specific sodium/proton exchanger (sNHE) …

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Interaction of MDIMP with the Voltage-Gated Calcium Channels

Juan de la Rosa Vázquez, Maricela García‐Castañeda, Takuya Nishigaki, Juan Carlos Gómora et autres

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CaV3.1 channel pore pseudo-symmetry revealed by selectivity filter mutations in its domains I/II

Edgar Garza-López, Andrés Aldana, Alberto Darszon, Takuya Nishigaki et autres

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