Eyemen Kheir

Enhancing hypercompact CasΦ2 activity through EPICA.2, an optimized eukaryotic directed evolution platform

Giulia Vittoria Ruta, Matteo Ciciani, Veronica De Sanctis, Roberto Bertorelli et autres

Compact Cas nucleases offer advantages over the widely used SpCas9 due to their smaller size, which enables more efficient delivery for in vivo applications. Among these, the phage-encoded CasΦ2 (Cas12j2) is highly promising due to its relaxed PAM requirement (5′-TTN-3′) and compact …

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Author Reply to Peer Reviews of CD90 identifies distinct fractions of muscle stem cells with different modalities of activation and quiescence maintenance

Eyemen Kheir, Michela Libergoli, Serena Di Savino, Samuele Metti et autres

it, dk, us, fr (code pays fourni par la source)

Abstract 7306: Does ribosomal protein haploinsufficiency induce cell competition in mammalian epithelia

Neha Joshi, Nicholas Baker, Eyemen Kheir

Abstract Cell competition is a mechanism in which cells can be eliminated by neighboring cells with higher cellular fitness. Cell competition was discovered in Drosophila where heterozygosity for Ribosomal protein (Rp) gene mutations leads to elimination by cell competition with wild type …

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CD90 identifies distinct fractions of muscle stem cells with different modalities of activation and quiescence maintenance

Eyemen Kheir, Michela Libergoli, Samuele Metti, Jonas Jensen et autres

SUMMARY Stem cell transition from quiescence to activation is crucial to guarantee productive tissue regeneration. Here we show that CD90 diversifies quiescent muscle stem cells (MuSCs) in murine and human muscle into two subpopulations differing in the kinetics of activation, CD90 +ve …

it, dk, us, fr (code pays fourni par la source)

WITHDRAWN: DNA polymerase λ-driven targeted mutagenesis for directed evolution in human cells

Davide Aiello, Matteo Ciciani, Federica Marelli, Marta Stancampiano et autres

Withdrawal Statement The authors have withdrawn this manuscript due to the need for further modifications in the experimental setup. Therefore, the authors do not wish this work to be cited as reference for this project. If you have any questions, please contact …

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Eukaryotic-driven directed evolution of Cas9 nucleases

Giulia Vittoria Ruta, Matteo Ciciani, Eyemen Kheir, Michele Domenico Gentile et autres

BACKGROUND: Further advancement of genome editing highly depends on the development of tools with higher compatibility with eukaryotes. A multitude of described Cas9s have great potential but require optimization for genome editing purposes. Among these, the Cas9 from Campylobacter jejuni, CjCas9, has …

it, fr (code pays fourni par la source)

Combinatorial activation of the WNT‐dependent fibrogenic program by distinct complement subunits in dystrophic muscle

Francesca Florio, Sara Vencato, Filomena Tiziana Papa, Michela Libergoli et autres

Fibrosis is associated with compromised muscle functionality in Duchenne muscular dystrophy (DMD). We report observations with tissues from dystrophic patients and mice supporting a model to explain fibrosis in DMD, which relies on the crosstalk between the complement and the WNT signaling …

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Eukaryotic-driven directed evolution of Cas9 nucleases

Giulia Vittoria Ruta, Matteo Ciciani, Eyemen Kheir, Michele Domenico Gentile et autres

ABSTRACT Full exploitation of the natural reservoir of CRISPR-Cas nucleases from prokaryotes for genome editing is limited by the suboptimal activity of these enzymes in mammalian cells. Here we developed a E ukaryotic P latform to Improve C as A ctivity (EPICA) …

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Translational enhancement by base editing of the Kozak sequence rescues haploinsufficiency

Chiara Ambrosini, Eliana Destefanis, Eyemen Kheir, Francesca Broso et autres

A variety of single-gene human diseases are caused by haploinsufficiency, a genetic condition by which mutational inactivation of one allele leads to reduced protein levels and functional impairment. Translational enhancement of the spare allele could exert a therapeutic effect. Here we developed …

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Generation of corrected hiPSC clones from a Cornelia de Lange Syndrome (CdLS) patient through CRISPR-Cas-based technology

Alessandro Umbach, Giulia Maule, Eyemen Kheir, Alessandro Cutarelli et autres

BACKGROUND: Cornelia de Lange syndrome (CdLS) is a rare multisystem genetic disorder which is caused by genetic defects involving the Nipped-B-like protein (NIPBL) gene in the majority of clinical cases (60-70%). Currently, there are no specific cures available for CdLS and clinical …

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Generation of corrected hiPSC clones from a Cornelia de Lange Syndrome (CdLS) patient through CRISPR-Cas based technology

Alessandro Umbach, Giulia Maule, Eyemen Kheir, Alessandro Cutarelli et autres

it (code pays fourni par la source)

Additional file 3 of Generation of corrected hiPSC clones from a Cornelia de Lange Syndrome (CdLS) patient through CRISPR-Cas-based technology

Alessandro Umbach, Giulia Maule, Eyemen Kheir, Alessandro Cutarelli et autres

Additional file 3. Data—GUIDEseq.

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