Roberto Bertorelli

Enhancing hypercompact CasΦ2 activity through EPICA.2, an optimized eukaryotic directed evolution platform

Giulia Vittoria Ruta, Matteo Ciciani, Veronica De Sanctis, Roberto Bertorelli et autres

Compact Cas nucleases offer advantages over the widely used SpCas9 due to their smaller size, which enables more efficient delivery for in vivo applications. Among these, the phage-encoded CasΦ2 (Cas12j2) is highly promising due to its relaxed PAM requirement (5′-TTN-3′) and compact …

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Somatic pathogenic mitochondrial DNA mutations independently predict worse prognosis in hepatocellular carcinoma

Camelia Alexandra Coadǎ, Monica De Luise, Rosanna Clima, Stefano Miglietta et autres

Hepatocellular carcinoma (HCC) is one of the deadliest cancers worldwide, but its cancerization and progression mechanisms are still poorly understood. Mitochondrial DNA (mtDNA) mutations have been involved in tumor progression by influencing metabolic rewiring and plasticity. In this work, we aimed to …

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Supplementary Data from Alpha-1 Adrenergic Antagonists Sensitize Neuroblastoma to Therapeutic Differentiation

Francesca Broso, Pamela Gatto, Viktoryia Sidarovich, Chiara Ambrosini et autres

Supplementary Figures from S1 to S23 and Supplementary tables from S1 to S3

Profibrotic Molecules Are Reduced in CRISPR-Edited Emery–Dreifuss Muscular Dystrophy Fibroblasts

Eleonora Cattin, Elisa Schena, Elisabetta Mattioli, Stefania Marcuzzo et autres

Emery–Dreifuss muscular dystrophy (EDMD) is caused by mutations in EMD, LMNA, SYNE1, SYNE2, and other related genes. The disease is characterized by joint contractures, muscle weakening and wasting, and heart conduction defects associated with dilated cardiomyopathy. Previous studies demonstrated the activation of …

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Author Reply to Peer Reviews of A TranSNP in the DDIT4 mRNA can impact its translation efficiency and modulate p53-dependent responses in cancer cells

Meriem Hadjer Hamadou, Laura Alunno, Tecla Venturelli, Samuel Valentini et autres

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A TranSNP in the DDIT4 mRNA can impact its translation efficiency and modulate p53-dependent responses in cancer cells

Meriem Hadjer Hamadou, Laura Alunno, Tecla Venturelli, Samuel Valentini et autres

Abstract Relatively few studies have examined the link between SNPs and mRNA translation, despite the established importance of translational regulation in shaping cell phenotypes. We developed a pipeline analyzing the allelic imbalance in total and polysome-bound mRNAs from paired RNA-seq data of …

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Restored Collagen VI Microfilaments Network in the Extracellular Matrix of CRISPR-Edited Ullrich Congenital Muscular Dystrophy Fibroblasts

Daniela Benati, Eleonora Cattin, Federico Corradi, Tommaso Micelli Ferrari et autres

Collagen VI is an essential component of the extracellular matrix (ECM) composed by α1, α2 and α3 chains and encoded by COL6A1, COL6A2 and COL6A3 genes. Dominant negative pathogenic variants in COL6A genes result in defects in collagen VI protein and are …

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Data from Alpha-1 adrenergic antagonists sensitize neuroblastoma to therapeutic differentiation

Francesca Broso, Pamela Gatto, Viktoryia Sidarovich, Chiara Ambrosini et autres

Abstract Neuroblastoma (NB) is an aggressive childhood tumor, with high-risk cases having a 5-year overall survival probability of approximately 50%. The multimodal therapeutic approach for NB includes treatment with the retinoid isotretinoin (13-cis retinoic acid; 13cRA), which is used in the post-consolidation …

Supplementary Data from Alpha-1 adrenergic antagonists sensitize neuroblastoma to therapeutic differentiation

Francesca Broso, Pamela Gatto, Viktoryia Sidarovich, Chiara Ambrosini et autres

Supplementary Figures from S1 to S23 and Supplementary tables from S1 to S3

Supplementary Data from Alpha-1 adrenergic antagonists sensitize neuroblastoma to therapeutic differentiation

Francesca Broso, Pamela Gatto, Viktoryia Sidarovich, Chiara Ambrosini et autres

Supplementary Figures from S1 to S23 and Supplementary tables from S1 to S3

WITHDRAWN: DNA polymerase λ-driven targeted mutagenesis for directed evolution in human cells

Davide Aiello, Matteo Ciciani, Federica Marelli, Marta Stancampiano et autres

Withdrawal Statement The authors have withdrawn this manuscript due to the need for further modifications in the experimental setup. Therefore, the authors do not wish this work to be cited as reference for this project. If you have any questions, please contact …

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Data from Alpha-1 Adrenergic Antagonists Sensitize Neuroblastoma to Therapeutic Differentiation

Francesca Broso, Pamela Gatto, Viktoryia Sidarovich, Chiara Ambrosini et autres

Abstract Neuroblastoma (NB) is an aggressive childhood tumor, with high-risk cases having a 5-year overall survival probability of approximately 50%. The multimodal therapeutic approach for NB includes treatment with the retinoid isotretinoin (13-cis retinoic acid; 13cRA), which is used in the post-consolidation …