Kasper C. D. Roet

A multiparametric activity profiling platform for neuron disease phenotyping and drug screening

Bruno Boivin, Kasper C. D. Roet, Xuan Huang, Kyle W. Karhohs et autres

Patient stem cell-derived models enable imaging of complex disease phenotypes and the development of scalable drug discovery platforms. Current preclinical methods for assessing cellular activity do not, however, capture the full intricacies of disease-induced disturbances and instead typically focus on a single …

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A Multiparametric Activity Profiling Platform for Neuron Disease Phenotyping and Drug Screening

Bruno Boivin, Kasper C. D. Roet, Xuan Huang, Kyle W. Karhohs et autres

ABSTRACT Patient stem cell-derived models enable imaging of complex disease phenotypes and the development of scalable drug discovery platforms. Current preclinical methods for assessing cellular activity do not, however, capture the full intricacies of disease-induced disturbances, and instead typically focus on a …

us (code pays fourni par la source)

Human amyotrophic lateral sclerosis excitability phenotype screen: Target discovery and validation

Xuan Huang, Kasper C. D. Roet, Liying Zhang, Amy L. Brault et autres

Drug development is hampered by poor target selection. Phenotypic screens using neurons differentiated from patient stem cells offer the possibility to validate known and discover novel disease targets in an unbiased fashion. To identify targets for managing hyperexcitability, a pathological feature of …

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Human Amyotrophic Lateral Sclerosis Excitability Phenotype Screen: Target Discovery and Validation

Xuan Huang, Kasper C. D. Roet, Liying Zhang, Amy L. Brault et autres

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A High-Content Screen Identifies TPP1 and Aurora B as Regulators of Axonal Mitochondrial Transport

Evgeny Shlevkov, Himanish Basu, Mark‐Anthony Bray, Zheng Sun et autres

Dysregulated axonal trafficking of mitochondria is linked to neurodegenerative disorders. We report a high-content screen for small-molecule regulators of the axonal transport of mitochondria. Six compounds enhanced mitochondrial transport in the sub-micromolar range, acting via three cellular targets: F-actin, Tripeptidyl peptidase 1 …

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A Role for Neuropilins in the Interaction between Schwann Cells and Meningeal Cells

Kasper C. D. Roet, Kerstin T.S. Wirz, Elske H. P. Franssen, Joost Verhaagen

In their natural habitat, the peripheral nerve, Schwann cells (SCs) form nicely aligned pathways (also known as the bands of Büngner) that guide regenerating axons to their targets. Schwann cells that are implanted in the lesioned spinal cord fail to align in …

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Understanding the neural repair-promoting properties of olfactory ensheathing cells

Kasper C. D. Roet, Joost Verhaagen

nl (code pays fourni par la source)

Lentiviral Vector-Mediated Gradients of GDNF in the Injured Peripheral Nerve: Effects on Nerve Coil Formation, Schwann Cell Maturation and Myelination

Ruben Eggers, Fred de Winter, Stefan A. Hoyng, Kasper C. D. Roet et autres

Although the peripheral nerve is capable of regeneration, only a small minority of patients regain normal function after surgical reconstruction of a major peripheral nerve lesion, resulting in a severe and lasting negative impact on the quality of life. Glial cell-line derived …

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A Multilevel Screening Strategy Defines a Molecular Fingerprint of Proregenerative Olfactory Ensheathing Cells and Identifies SCARB2, a Protein That Improves Regenerative Sprouting of Injured Sensory Spinal Axons

Kasper C. D. Roet, Elske H. P. Franssen, F.M. de Bree, Anke H. W. Essing et autres

Olfactory ensheathing cells (OECs) have neuro-restorative properties in animal models for spinal cord injury, stroke, and amyotrophic lateral sclerosis. Here we used a multistep screening approach to discover genes specifically contributing to the regeneration-promoting properties of OECs. Microarray screening of the injured …

nl (code pays fourni par la source)

Noninvasive Bioluminescence Imaging of Olfactory Ensheathing Glia and Schwann Cells following Transplantation into the Lesioned Rat Spinal Cord

Kasper C. D. Roet, Ruben Eggers, Joost Verhaagen

In this study, we assess the feasibility of bioluminescence imaging to monitor the survival of Schwann cells (SCs) and olfactory ensheathing glia cells (OECs) after implantation in the lesioned spinal cord of adult rats. To this end, purified SCs and OECs were …

nl (code pays fourni par la source)

Conditioning lesions before or after spinal cord injury recruit broad genetic mechanisms that sustain axonal regeneration: Superiority to camp-mediated effects

Armin Blesch, Paul P. Y. Lu, Shingo Tsukada, Laura Taylor Alto et autres

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Lentiviral vectors express chondroitinase ABC in cortical projections and promote sprouting of injured corticospinal axons

Rongrong Zhao, Elizabeth M. Muir, João Nuno Alves, Hannah M. Rickman et autres

Several diseases and injuries of the central nervous system could potentially be treated by delivery of an enzyme, which might most effectively be achieved by gene therapy. In particular, the bacterial enzyme chondroitinase ABC is beneficial in animal models of spinal cord …

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