Accès ouvert
2026
article
OpenAlex
Joshua I. Santiago, Ishtiyaq Ahmed, Jeanette Hahn, Abigail Rubino et autres
Natural transformation drives the spread of antibiotic resistance among bacteria. The DNA receptor ComEA is essential for transporting external transforming DNA into the periplasm by an unknown mechanism. Here, single-molecule optical tweezers and electron microscopy approaches show that Geobacillus stearothermophilus ComEA forms …
us
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Accès ouvert
2025
article
OpenAlex
Andrew M. Guarnaccia, Anjali Steenhaut, Sandra D. Olenic, J.-R. Na et autres
Ethanolamine signaling through the transmembrane quorum-sensing receptor CqsR influences Vibrio cholerae niche recognition and host colonization. In this study, we present a comprehensive structure-function analysis of CqsR. Specifically, we have determined X-ray crystal structures of the CqsR periplasmic domain bound to the …
us
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Accès ouvert
2025
preprint
OpenAlex
Joshua I. Santiago, Ishtiyaq Ahmed, Jeanette Hahn, David A. Dubnau et autres
ABSTRACT Natural transformation drives the spread of antibiotic resistance among bacteria. The DNA receptor ComEA is essential for transporting external transforming DNA into the periplasm by an unknown mechanism. Here, single-molecule optical tweezers and electron microscopy approaches show that Geobacillus stearothermophilus ComEA …
us
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Accès ouvert
2025
preprint
OpenAlex
Vedang Warapande, Fanwang Meng, Alexandra Bozan, David Graff et autres
ABSTRACT Drug discovery is inherently challenged by a multiple criteria decision making problem. The arduous path from hit discovery through lead optimization and preclinical candidate selection necessitates the evolution of a plethora of molecular properties. In this study, we focus on the …
us
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Accès ouvert
2025
conference-abstract
OpenAlex
Joshua I. Santiago, Ishtiyaq Ahmed, David A. Dubnau, Matthew B. Neiditch et autres
us
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Accès ouvert
2025
conference-abstract
OpenAlex
Joshua I. Santiago, Ishtiyaq Ahmed, David A. Dubnau, Matthew B. Neiditch et autres
us
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Accès ouvert
2023
article
OpenAlex
Andrew M. Guarnaccia, Wei-Lung Ng, Anjali Steenhaut, Sandra D. Olenic et autres
OBJECTIVES/GOALS: Our goal is to determine the structure of the CACHE domain of the Vibrio cholerae quorum sensing receptor CqsR as well as its autoinducer (AI). We are performing X-ray crystallography on the protein in its apo form, with the fractions containing …
nl, us
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Accès ouvert
2023
erratum
OpenAlex
Andrew M. Guarnaccia, Wai‐Leung Ng, Anjali Steenhaut, Sandra D. Olenic et autres
[This corrects the article DOI: 10.1017/cts.2023.503.].
us
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Accès ouvert
2022
article
OpenAlex
Ishtiyaq Ahmed, Jeanette Hahn, Amy Henrickson, Faisal Tarique Khaja et autres
An essential step in bacterial transformation is the uptake of DNA into the periplasm, across the thick peptidoglycan cell wall of Gram-positive bacteria, or the outer membrane and thin peptidoglycan layer of Gram-negative bacteria. ComEA, a DNA-binding protein widely conserved in transformable …
us, ca
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Accès ouvert
2022
article
OpenAlex
Rudraraju Srilakshmi Reshma, Samer S. Daher, Ricardo Gallardo‐Macias, Xin Wang et autres
Recent studies have reported the β-ketoacyl-acyl carrier protein KasA as a druggable target for Mycobacterium tuberculosis. This review summarizes the current status of major classes of KasA inhibitors with an emphasis on significant contributions from structure-based design methods leveraging X-ray crystal structures …
us
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Accès ouvert
2022
article
OpenAlex
Brian Y. Hsueh, Geoffrey B. Severin, Clinton A. Elg, Evan J. Waldron et autres
us
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Accès ouvert
2021
article
OpenAlex
Catherine A. Klancher, G. Minasov, Ram C. Podicheti, Douglas B. Rusch et autres
, the transcription factor ChiS directly regulates gene expression through a cryptic DNA-binding domain. This domain lacked homology to any known DNA-binding protein. In the current study, we determined the structure of the ChiS DNA-binding domain (DBD) and found that the ChiS-family …
us
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