Alan John Slusarenko

In Vivo Antitumor Activity of Allicin in a Pediatric Neuroblastoma Patient-derived Xenograft (PDX) Mouse Model

Chad R. Schultz, Martin C. H. Gruhlke, Alan John Slusarenko, André S. Bachmann

Background/Aim: Allicin is a small-molecule natural product found in garlic (Allium sativum). We previously showed that allicin inhibits ornithine decarboxylase (ODC) in vitro and induces apoptotic cell death in pediatric neuroblastoma (NB) cancer cell cultures. However, its potency as an anticancer agent …

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Combating Black Fungus: Using Allicin as a Potent Antifungal Agent against Mucorales

Christina Schier, Martin C. H. Gruhlke, Georg Reucher, Alan John Slusarenko et autres

Invasive fungal (IF) diseases are a leading global cause of mortality, particularly among immunocompromised individuals. The SARS-CoV-2 pandemic further exacerbated this scenario, intensifying comorbid IF infections such as mucormycoses of the nasopharynx. In the work reported here, it is shown that zygomycetes, …

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Mode of Action of the Natural Product Allicin in a Plant Model: Influence on the Cytoskeleton and Subsequent Shift in Auxin Localization

Ulrike Noll, Miriam Schreiber, Monika Hermanns, Christopher A. Mertes et autres

Allicin is a defense substance produced by garlic cells when they are injured. It is a redox-active thiosulfinate showing redox-activity with a broad range of dose-dependent antimicrobial and biocidal activity. It is known that allicin efficiently oxidizes thiol-groups, and it has been …

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Two-component carnitine monooxygenase from Escherichia coli: functional characterization, inhibition and mutagenesis of the molecular interface

Fabian Piskol, Kerstin Neubauer, M. Eggers, Lisa Margarete Bode et autres

Gut microbial production of trimethylamine (TMA) from l-carnitine is directly linked to cardiovascular disease. TMA formation is facilitated by carnitine monooxygenase, which was proposed as a target for the development of new cardioprotective compounds. Therefore, the molecular understanding of the two-component Rieske-type …

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Seeing the smell of garlic: Detection of gas phase volatiles from crushed garlic (Allium sativum), onion (Allium cepa), ramsons (Allium ursinum) and human garlic breath using SESI-Orbitrap MS

Hendrik G. Mengers, Christina Schier, Martín Zimmermann, Martin C. H. Gruhlke et autres

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Allicin as a Volatile or Nebulisable Antimycotic for the Treatment of Pulmonary Mycoses: In Vitro Studies Using a Lung Flow Test Rig

Christina Schier, Jana Foerster, Monika Heupel, Philipp Dörner et autres

Fungal infections of the lung are an increasing problem worldwide and the search for novel therapeutic agents is a current challenge due to emerging resistance to current antimycotics. The volatile defence substance allicin is formed naturally by freshly injured garlic plants and …

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The Effect of Allicin on the Proteome of SARS-CoV-2 Infected Calu-3 Cells

Kirstin Mösbauer, Verena Nadin Fritsch, Lorenz Adrian, Jörg Bernhardt et autres

-thioallylation of protein thiols and low molecular weight thiols. Here, we investigated the effect of allicin on SARS-CoV-2 infected Vero E6 and Calu-3 cells. Toxicity tests revealed that Calu-3 cells showed greater allicin tolerance, probably due to >4-fold higher GSH levels compared …

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The two-Cys-type TetR repressor GbaA confers resistance under disulfide and electrophile stress in Staphylococcus aureus

Vu Van Loi, Tobias Busche, Verena Nadin Fritsch, Christoph Weise et autres

Staphylococcus aureus has to cope with oxidative and electrophile stress during host-pathogen interactions. The TetR-family repressor GbaA was shown to sense electrophiles, such as N-ethylmaleimide (NEM) via monothiol mechanisms of the two conserved Cys55 or Cys104 residues in vitro. In this study, …

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Allicin inhibits SARS-CoV-2 replication and abrogates the antiviral host response in the Calu-3 proteome

Kirstin Mösbauer, Verena Nadin Fritsch, Lorenz Adrian, Jörg Bernhardt et autres

ABSTRACT The Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) pandemic is a major health burden. Volatile garlic organosulfur compounds, such as the thiol-reactive allicin (diallyl thiosulfinate) exert strong antimicrobial activity against various respiratory pathogens. Here, we investigated the antiviral activity of allicin …

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Allicin, the Odor of Freshly Crushed Garlic: A Review of Recent Progress in Understanding Allicin’s Effects on Cells

Jan Borlinghaus, Jana Foerster, Ulrike Kappler, Haike Antelmann et autres

The volatile organic sulfur compound allicin (diallyl thiosulfinate) is produced as a defense substance when garlic (Allium sativum) tissues are damaged, for example by the activities of pathogens or pests. Allicin gives crushed garlic its characteristic odor, is membrane permeable and readily …

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Allicin From Garlic Disrupts the Cytoskeleton in Plants

Ulrike Noll, Miriam Schreiber, Monika Hermanns, Christopher A. Mertes et autres

Abstract Allicin is a defence substance produced by garlic cells upon injury. It is a thiosulfinate showing redox-activity and a broad range of antimicrobial and biocidal activity. It is known that allicin efficiently oxidizes thiol-groups and it has been described as a …

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The Sulfilimine Analogue of Allicin, S-Allyl-S-(S-allyl)-N-Cyanosulfilimine, Is Antimicrobial and Reacts with Glutathione

Tobias Horn, Wolfgang Bettray, Ulrike Noll, Felix Krauskopf et autres

When cells of garlic (Allium sativum) are disrupted by wounding, they produce the defense substance allicin (diallylthiosulfinate). Allicin is an efficient thiol trap and readily passes through cell membranes into the cytosol, where it behaves as a redox toxin by oxidizing the …

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