Naoya Ohara

MetF‐Dependent Methionine Biosynthesis Is Required for Mycobacterium tuberculosis Survival and In Vivo Persistence

Ikue Tosa, Tomoki Kitahara, Katsuki Takebe, Masaaki Nakayama et autres

ABSTRACT Methionine biosynthesis is essential for Mycobacterium tuberculosis , but the in vivo relevance of individual enzymes remains unclear. We performed transposon sequencing in Mycobacterium bovis BCG under methionine‐free conditions and identified metF as a major fitness determinant. To define enzyme‐specific functions, …

jp, id (code pays fourni par la source)

High-throughput screening system for antimycobacterial compounds using in vitro infected cells: construction and application to compounds in the Ōmura Natural Compound Library

Takemasa Takii, Aoi Kimishima, Masato Iwatsuki, Yoshihiro Watanabe et autres

ABSTRACT The global increase in multidrug-resistant tuberculosis strains poses a major threat; consequently, there is an urgent need to identify and develop novel drugs. We have previously presented a simple fibroblast assay (SFA) screening method that enables the simultaneous assessment of antibacterial …

jp (code pays fourni par la source)

Muse cell known as a kind of Stem cell enabled therapy for SARS-CoV-2-Lung and Olfactory Impairment in Syrian Hamsters and may also have therapeutic effects for long COVID

Jun Fujii, Satoshi Shibata, Hiroshi Ito, Toshihiro Ito et autres

jp (code pays fourni par la source)

Calcium ions play a critical role in calcification of Corynebacterium matruchotii

Naoko Ohara, Midori Ogawa, Katsuki Takebe, Ikue Tosa et autres

Dental calculus is a hardened deposit composed of calcium phosphate precipitated within dental plaque. While the involvement of dental calculus in the progression of periodontal disease is well established, many aspects of its formation process remain poorly understood. In this study, we …

jp (code pays fourni par la source)

Phenotypic Characterization of Porphyromonas gulae Fimbria Mutants

Hiroaki Inaba, Sho Yoshida, Naoya Ohara, Ryota Nomura et autres

Porphyromonas gulae has been established as a pathogen in animal periodontal disease and expresses several virulence factors, including fimbriae, lipopolysaccharide, and proteases. P. gulae fimbriae are classified into three genotypes (A, B, and C) based on the fimbrillin ( fimA ) gene …

Phenotypic Characterization of Porphyromonas gulae Fimbria Mutants

Hiroaki Inaba, Sho Yoshida, Naoya Ohara, Ryota Nomura et autres

Porphyromonas gulae has been established as a pathogen in animal periodontal disease and expresses several virulence factors, including fimbriae, lipopolysaccharide, and proteases. P. gulae fimbriae are classified into three genotypes (A, B, and C) based on the fimbrillin (fimA) gene encoding a …

jp (code pays fourni par la source)

Evaluation of Mycobacterium-derived plasmids for application in oral Actinomyces species

Sakiko Ohara, Yijuan Sheng, Yuki Nishiya, Ikue Tosa et autres

OBJECTIVES: Genetic manipulation tools are essential for elucidating the pathogenic mechanisms of microorganisms. Several species of Actinomyces, including A. israelii, are present in the oral cavity and they are the causative agents of actinomycosis. However, efficient gene-editing tools for these species have …

jp (code pays fourni par la source)

Analysis of the drug target of the anti-tuberculosis compound OCT313: phosphotransacetylase is a potential drug target for anti-mycobacterial agents

Takemasa Takii, Tomohiro Hasegawa, Saotomo Itoh, Shinji Maeda et autres

ABSTRACT Tuberculosis (TB) is one of the most common infectious diseases caused by bacteria worldwide. The increasing prevalence of multidrug-resistant TB (MDR-TB) and latent TB infection (LTBI) has intensified the global TB burden. Therefore, the development of new drugs for MDR-TB and …

jp, id, fr (code pays fourni par la source)

PGN_0298 in the Assembly and Insertion Machinery (Aim) Operon Is Essential for the Viability of Porphyromonas gingivalis

Sadamu Ono, Katsuki Takebe, Ikue Tosa, Yuki Nishiya et autres

Porphyromonas gingivalis is a typical periodontal pathogen, and one of its key virulence factors is the powerful protease gingipains. Gingipains are secreted via the type IX secretion system (T9SS) and are associated with the assembly and insertion machinery (Aim) operon (PGN_0296 to …

jp (code pays fourni par la source)

Mycobacterium tuberculosis bacillus induces pyroptosis in human lung fibroblasts

Takemasa Takii, Hiroyuki Yamada, Chihiro Motozono, Sho Yamasaki et autres

ABSTRACT We previously reported that live, but not dead, virulent Mycobacterium tuberculosis ( Mtb ) H37Rv bacilli induce cell death in human lung fibroblast cell lines, MRC-5, MRC-9, and TIG-1. Here, using two distinct Mtb strains from two different lineages (HN878 lineage …

jp, us (code pays fourni par la source)

BMP-2 generates a functional bone marrow niche by inducing the differentiation of local mesenchymal cells into CAR cells

Ikue Tosa, Mitsuaki Ono, Anh Tuan Dang, Ziyi Wang et autres

ABSTRACT: Ectopic bone marrow (eBM) holds tremendous potential as an artificial organ, serving not only in stem cell transplantation therapies but also as a controlled experimental system for analyzing cellular dynamics and interactions between cells and the matrix during the formation, maintenance, …

jp (code pays fourni par la source)

Rescue from Stx2-Producing E. coli-Associated Encephalopathy by Intravenous Injection of Muse Cells in NOD-SCID Mice

Ryo Ozuru, Shohei Wakao, Takahiro Tsuji, Naoya Ohara et autres