Phenotypic Characterization of Porphyromonas gulae Fimbria Mutants
Rattachement africain : jp. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Porphyromonas gulae has been established as a pathogen in animal periodontal disease and expresses several virulence factors, including fimbriae, lipopolysaccharide, and proteases. P. gulae fimbriae are classified into three genotypes (A, B, and C) based on the fimbrillin (fimA) gene encoding a fimbrial subunit protein. We previously reported that P. gulae organisms with type C fimbriae were clearly more virulent as compared to those with other fimbria types, though the basic function of this is not fully understood. Here, we investigated the function of fimbriae using fimA-deficient mutants. In the present study, bacterial fimbriae were related to biofilm tenacity, whereas biofilm formation was not. Also, P. gulae fimbriae are involved in bacterial adhesion and entry into gingival epithelial cells. The role of P. gulae fimbriae in bacterial pathogenicity was then determined using fimA-deficient mutants with a silkworm infection model. Additionally, P. gulae infection can induce inflammatory responses in gingival epithelial cells and also inhibit cell migration and proliferation. In contrast, a P. gulae fimA-deficient mutant did not cause several of those cellular responses. Together, the results show that fimbriae contribute to the pathogenicity of P. gulae by affecting bacterial functions and host cell interactions.
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Okayama University Department of Pediatric Dentistry pays non établi dans la noticeUniversité ou école supérieure
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Hiroshima University pays non établi dans la noticeUniversité ou école supérieure
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Osaka University of Economics pays non établi dans la noticeUniversité ou école supérieure
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Osaka City University pays non établi dans la noticeUniversité ou école supérieure
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The University of Osaka pays non établi dans la noticeUniversité ou école supérieure
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Dentistry and Pharmaceutical Sciences pays non établi dans la noticeInstitution
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Graduate School of Biomedical and Health Sciences Department of Pediatric Dentistry pays non établi dans la noticeUniversité ou école supérieure
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Graduate School of Dentistry Department of Pediatric Dentistry pays non établi dans la noticeUniversité ou école supérieure
Department of Pediatric Dentistry — Okayama University, Hiroshima University et Osaka University of Economics, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.